: The effects of foliar spraying with spermidine (Spd), ranging in concentration from 0.25 to 0.50 mmol/L, on the antioxidant system under Cd2+ stress (range 0.1–0.2 mmol/L Cd2+) in Typha latifolia L. grown hydroponi...: The effects of foliar spraying with spermidine (Spd), ranging in concentration from 0.25 to 0.50 mmol/L, on the antioxidant system under Cd2+ stress (range 0.1–0.2 mmol/L Cd2+) in Typha latifolia L. grown hydroponically were investigated in order to offer a referenced evidence for an understanding of the mechanism by which polyamines (PAs) relieve the damage to plants by heavy metal and improve the phytoremediation efficiency of heavy metal-contaminated water. The results showed that Cd2+ stress induced oxidative injury, as evidenced by an increase in the generation of superoxide anion (O2-), as well as the hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents in both leaves and caudices. With the exception of superoxide dismutase (SOD) activity in the leaves, an increase in the activities of catalase (CAT), guaiacol peroxidase (GPX), and glutathione reductase (GR) was observed in both leaves and caudices, SOD activity was increased in caudices, and ascorbate peroxidase (APX) activity was increased in leaves following Cd2+ treatment. The reduced glutathione (GSH) content in both leaves and caudices and the reductive ascorbate content in leaves was obviously increased, which were prompted by the application of exogenous Spd. Spraying with Spd increased the activity of GR and APX in both leaves and caudices, whereas the activity of SOD, CAT, and GPX was increased only in caudices following spraying with Spd. The generation of O2 -. and the H2O2 and MDA content in both leaves and caudices decreased after spraying with Spd. The decrease in MDA was more obvious following the application of 0.25 than 0.50 mmol/L Spd. It is supposed that exogenous Spd elevated the tolerance of T. latifolia under Cd2+ stress primarily by increasing GR activity and the GSH level.展开更多
Objective:To evaluate the phytochemical constituents and antioxidant activities of aqueous extract of Scholia latifolia(S.latifolia)bark locally used for the treatment of oxidative stress-induced ailments in South Afr...Objective:To evaluate the phytochemical constituents and antioxidant activities of aqueous extract of Scholia latifolia(S.latifolia)bark locally used for the treatment of oxidative stress-induced ailments in South Africa.Methods:The antioxidant and free radical scavenging activity of aqueous extract of the plant was assessed against 1,1-diphenyl-2-pierylhydrazyl(DPPH),nitric oxide(NO),2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)and the ferric reducing agent.Total phenolics,flavonoids,flavonols and proanthocyanidins were also determined to assess their corresponding effect on the antioxidant activity of this plant.Results:The activities of plant extract against DPPH,ABTS and NO radicals were concentration dependent with IC_(50)value of 0.06,0.05 and 0.05 mg/mL,respectively.The reducing power of the extract was greater than that of butylated hydroxyl toluene(BHT)and ascorbic acid which were used as standard drugs in a concentration dependent manner.The total phenolics content of the aqueous bark extract was(193.33±0.03 TE/g),followed by flavonoids(72.70±0.01 QE/g),proanthocyanidins|48.76±0.00 CE/g)and flavonols(47.76±0.21 QE/g).Phytochemical analysis revealed the presence of percentage tannin(11.40±0.02),alkaloid(9.80±0.01),steroids(18.20±0.01),glycosides(29.80±0.01)and saponins(6.80±0.00).The results exhibited a positive linear correlation between these polyphenols and the free radical scavenging activities.Conclusions:Our findings provide evidence that the crude aqueous extract of S.latifolia is a potential source of natural antioxidants and this justifies its uses in folkloric medicines.展开更多
Objective:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Scholia latifolia（S.latifolia） bark commonly used in South Africa traditional medicine for the treatment of various ...Objective:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Scholia latifolia（S.latifolia） bark commonly used in South Africa traditional medicine for the treatment of various ailments.Methods:The antibacterial test and MIC was determined by using agar well diffusion and dilution methods respectively against eight strains of bacteria. The total phenol,proanthocyanidin and flavonoid contents of 5.latifolia were assessed using Standard methods.The antioxidant activity of the extract was evaluated using ferric reducing power and the free radical scavenging activity against 1.1-diphenyl-2-picrylhydrazyl（DPPH）, 2.2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid（ABTS）,nitric oxide（NO）,hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>） and lipid oxidation（LO）.Results:The antibacterial activity demonstrated an appreciable effect against all the gram positive bacteria at MIC between 0.016 and 10 mg/ml. while that of gram negative bacteria was above 10 mg/mL.The plant extract exhibited high concentration of proanthocyanidin[（300.00±0.10） mg CE/g].followed by flavonoid[（12.46±0.04 mg） TE/g]and phenol[（1 l.06±0.03） mg QE/g]contents.Similarly,the extract at 0.5 mg/mL scavenges DPPH.ABTS.H<sub>2</sub>O<sub>2</sub>.LO and NO by 87.55%.89.47%.77.15%.86.48%and 77.75%of the radicals respectively.The reducing power was also found to be concentration dependent Conclusions: Our data suggest that S.latifolia extract has antibacterial and antioxidants activity and thus could be used as alternative therapv against antibiotic resistance bacteria and to prevent man) radical related diseases.展开更多
[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a prob...[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.展开更多
Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha lat...Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha latifolia were collected in Liberty State Park during the growing season（May–September）in 2011 at two sites with the high and low metal loads,respectively.The objective of this study was to understand the metal（Fe,Mn,Cu,Pb and Zn）concentration and spatial distributions in P.australis and T.latifolia root systems with micro-meter scale resolution using synchrotron X-ray microfluorescence（μXRF）and synchrotron X-ray computed microtomography（μCMT）techniques.The root structure measurement by synchrotron μCMT showed that high X-ray attenuation substance appeared in the epidermis.Synchrotron μXRF measurement showed that metal concentrations and distributions in the root cross-section between epidermis and vascular tissue were statistically different.Significant correlations were found between metals（Cu,Mn,Pb and Zn）and Fe in the epidermis,implying that metals were scavenged by Fe oxides.The results from this study suggest that the expression of metal transport and accumulation within the root systems may be element specific.The information derived from this study can improve our current knowledge of the wetland plant ecological function in brownfield remediation.展开更多
The objective of this study consisted of evaluating the effect of the application of chitosan(Q),1-methylcyclopropene(1-MCP)under two controlled temperatures on some physical,physiological,and chemical parameters cont...The objective of this study consisted of evaluating the effect of the application of chitosan(Q),1-methylcyclopropene(1-MCP)under two controlled temperatures on some physical,physiological,and chemical parameters contributing to the quality of Persian lime Citrus latifolia fruits.Eight treatments were evaluated,resulting from the combination of four senescence retardant applications(Q,1-MCP,1-MCP+Q,and without application)on fruits stored at two temperatures(12/20℃.Epidermis color(luminosity,chromaticity,and hue),fruit appearance,respiration,weight loss,total juice content,total soluble solids,and titratable acidity were registered during the study.The results indicate that the 1-MCP and Q combination favored fruit postharvest quality conditions such as reducing respiration rate;maintaining fruit good appearance,turgidity,and brighter fruit chromaticity;fruits treated with chitosan and stored at 12℃,favored total soluble solids.On the other hand,Variables such as weight loss,juice content,acidity,phenols,flavonoids,antioxidant activity,and hue values were favored by a 12℃ storing temperature regardless of the treatment.In general,the use of modified atmospheres,senescence retardants,and refrigeration as postharvest methods,can increase Persian lime shelf life.展开更多
The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good vis...The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good visual appearance of fresh Z. latifolia, inhibited browning, mildew and weight loss at the bottom of Z. latifolia, and there is no significant changes on L, a*, b* and ΔE during the whole storage period. In addition, 1-MCP treatment inhibited the respiratory intensity of Z. latifolia during the first three days of storage, but it was significantly higher than that of the control on the sixth day of storage. Compared with the control, 1-MCP treatment maintained relatively high SOD, CAT, APX activities and low PAL, POD, PLD, lipase and LOX activities, delayed the decline of AsA content, reduced the accumulation of O<sub>2</sub><sup>-</sup>, H<sub>2</sub>O<sub>2</sub> and MDA, and ultimately maintained the integrity of cell structure and delayed the senescence of Z. latifolia. In addition, positive effects of 1-MCP on maintaining the cell structure integrity were observed in this investigation throughout the storage period at 25˚C.展开更多
[Objective]The paper was to normalize the application of fenaminosulf in single cropping Zizania latifolia. [Method]Effects of spraying periods,different concentrations and spraying times of fenaminosulf on yield and ...[Objective]The paper was to normalize the application of fenaminosulf in single cropping Zizania latifolia. [Method]Effects of spraying periods,different concentrations and spraying times of fenaminosulf on yield and output value of single cropping Z. latifolia were studied.[Result]In some regions where single cropping Z. latifolia could be harvested twice per year,fenaminosulf significantly promoted formation of swollen gall,and advanced the harvest period for about 50 d. Application of 0.5 g/L（ a.i.） fenaminosulf at 150,160 and 175 d post transplanting promoted swollen gall formation and enhanced the yield of Z. latifolia.In normal single cropping Z. latifolia growing regions,once application of fenaminosulf obviously advanced harvest period and improved yield and benefit of Z. latifolia,but with the increasing frequency of use,yield and benefit of Z. latifolia decreased significantly.[Conclusion]It is recommended that 1.0 g/L（a.i.） fenaminosulf can be applied for just once at 40-60 d post transplanting of Z. latifolia.展开更多
文摘: The effects of foliar spraying with spermidine (Spd), ranging in concentration from 0.25 to 0.50 mmol/L, on the antioxidant system under Cd2+ stress (range 0.1–0.2 mmol/L Cd2+) in Typha latifolia L. grown hydroponically were investigated in order to offer a referenced evidence for an understanding of the mechanism by which polyamines (PAs) relieve the damage to plants by heavy metal and improve the phytoremediation efficiency of heavy metal-contaminated water. The results showed that Cd2+ stress induced oxidative injury, as evidenced by an increase in the generation of superoxide anion (O2-), as well as the hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents in both leaves and caudices. With the exception of superoxide dismutase (SOD) activity in the leaves, an increase in the activities of catalase (CAT), guaiacol peroxidase (GPX), and glutathione reductase (GR) was observed in both leaves and caudices, SOD activity was increased in caudices, and ascorbate peroxidase (APX) activity was increased in leaves following Cd2+ treatment. The reduced glutathione (GSH) content in both leaves and caudices and the reductive ascorbate content in leaves was obviously increased, which were prompted by the application of exogenous Spd. Spraying with Spd increased the activity of GR and APX in both leaves and caudices, whereas the activity of SOD, CAT, and GPX was increased only in caudices following spraying with Spd. The generation of O2 -. and the H2O2 and MDA content in both leaves and caudices decreased after spraying with Spd. The decrease in MDA was more obvious following the application of 0.25 than 0.50 mmol/L Spd. It is supposed that exogenous Spd elevated the tolerance of T. latifolia under Cd2+ stress primarily by increasing GR activity and the GSH level.
基金supported by Govan Mbeki Research Development Centre of the University of Fort Hare
文摘Objective:To evaluate the phytochemical constituents and antioxidant activities of aqueous extract of Scholia latifolia(S.latifolia)bark locally used for the treatment of oxidative stress-induced ailments in South Africa.Methods:The antioxidant and free radical scavenging activity of aqueous extract of the plant was assessed against 1,1-diphenyl-2-pierylhydrazyl(DPPH),nitric oxide(NO),2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)and the ferric reducing agent.Total phenolics,flavonoids,flavonols and proanthocyanidins were also determined to assess their corresponding effect on the antioxidant activity of this plant.Results:The activities of plant extract against DPPH,ABTS and NO radicals were concentration dependent with IC_(50)value of 0.06,0.05 and 0.05 mg/mL,respectively.The reducing power of the extract was greater than that of butylated hydroxyl toluene(BHT)and ascorbic acid which were used as standard drugs in a concentration dependent manner.The total phenolics content of the aqueous bark extract was(193.33±0.03 TE/g),followed by flavonoids(72.70±0.01 QE/g),proanthocyanidins|48.76±0.00 CE/g)and flavonols(47.76±0.21 QE/g).Phytochemical analysis revealed the presence of percentage tannin(11.40±0.02),alkaloid(9.80±0.01),steroids(18.20±0.01),glycosides(29.80±0.01)and saponins(6.80±0.00).The results exhibited a positive linear correlation between these polyphenols and the free radical scavenging activities.Conclusions:Our findings provide evidence that the crude aqueous extract of S.latifolia is a potential source of natural antioxidants and this justifies its uses in folkloric medicines.
基金National Research Foundation(NRF)Govan Mbeki Research Development Centre(GMRDC) of University of Fort Hare for financial support
文摘Objective:To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Scholia latifolia（S.latifolia） bark commonly used in South Africa traditional medicine for the treatment of various ailments.Methods:The antibacterial test and MIC was determined by using agar well diffusion and dilution methods respectively against eight strains of bacteria. The total phenol,proanthocyanidin and flavonoid contents of 5.latifolia were assessed using Standard methods.The antioxidant activity of the extract was evaluated using ferric reducing power and the free radical scavenging activity against 1.1-diphenyl-2-picrylhydrazyl（DPPH）, 2.2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid（ABTS）,nitric oxide（NO）,hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>） and lipid oxidation（LO）.Results:The antibacterial activity demonstrated an appreciable effect against all the gram positive bacteria at MIC between 0.016 and 10 mg/ml. while that of gram negative bacteria was above 10 mg/mL.The plant extract exhibited high concentration of proanthocyanidin[（300.00±0.10） mg CE/g].followed by flavonoid[（12.46±0.04 mg） TE/g]and phenol[（1 l.06±0.03） mg QE/g]contents.Similarly,the extract at 0.5 mg/mL scavenges DPPH.ABTS.H<sub>2</sub>O<sub>2</sub>.LO and NO by 87.55%.89.47%.77.15%.86.48%and 77.75%of the radicals respectively.The reducing power was also found to be concentration dependent Conclusions: Our data suggest that S.latifolia extract has antibacterial and antioxidants activity and thus could be used as alternative therapv against antibiotic resistance bacteria and to prevent man) radical related diseases.
文摘[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.
基金supported in part by the Margaret and Herman Sokol Foundation(HF)China Scholarship Council(YQ)+6 种基金the State Key Laboratory of Estuarine and Coastal Research Open Research Fund(Ref #:SKLEC-KF201304)(HF,WZ,LY,YQ)supported in part by the U.S.Department of Energy,Office of Science,Office of Workforce Development for Teachers and Scientists(WDTS)under the Visiting Faculty Program(VFP)(HF)Portions of this work were performed at Beamline X27A,National Synchrotron Light Source(NSLS),and Biosciences Department,Brookhaven National LaboratoryWork in Bioscience Department,BNL,was partially supported by the Division of Chemical Sciences,Geosciences,and Biosciences,Office of Basic Energy Sciences of the US Department of Energy through Grant DEAC0298CH10886the National Science Foundation through grant MCB-1051675(CJL)X27A is supported in part by the U.S.Department of Energy--Geosciences(DE-FG02-92ER14244 to The University of Chicago-CARS)Use of the NSLS was supported by the U.S.Department of Energy,Office of Science,Office of Basic Energy Sciences,under Contract No.DE-AC02-98CH10886
文摘Liberty State Park in New Jersey,USA,is a ＂brownfield＂ site containing various levels of contaminants.To investigate metal uptake and distributions in plants on the brownfield site,Phragmites australis and Typha latifolia were collected in Liberty State Park during the growing season（May–September）in 2011 at two sites with the high and low metal loads,respectively.The objective of this study was to understand the metal（Fe,Mn,Cu,Pb and Zn）concentration and spatial distributions in P.australis and T.latifolia root systems with micro-meter scale resolution using synchrotron X-ray microfluorescence（μXRF）and synchrotron X-ray computed microtomography（μCMT）techniques.The root structure measurement by synchrotron μCMT showed that high X-ray attenuation substance appeared in the epidermis.Synchrotron μXRF measurement showed that metal concentrations and distributions in the root cross-section between epidermis and vascular tissue were statistically different.Significant correlations were found between metals（Cu,Mn,Pb and Zn）and Fe in the epidermis,implying that metals were scavenged by Fe oxides.The results from this study suggest that the expression of metal transport and accumulation within the root systems may be element specific.The information derived from this study can improve our current knowledge of the wetland plant ecological function in brownfield remediation.
基金funded by the National Institute of Forestry,Agriculture and Livestock Research(INIFAP)of MéxicoINIFAP provided the funds for research Project Number 14574034553“Evaluation of Rootstocks and Physiology of Citrus Production in Morelos,Guerrero and Oaxaca”.
文摘The objective of this study consisted of evaluating the effect of the application of chitosan(Q),1-methylcyclopropene(1-MCP)under two controlled temperatures on some physical,physiological,and chemical parameters contributing to the quality of Persian lime Citrus latifolia fruits.Eight treatments were evaluated,resulting from the combination of four senescence retardant applications(Q,1-MCP,1-MCP+Q,and without application)on fruits stored at two temperatures(12/20℃.Epidermis color(luminosity,chromaticity,and hue),fruit appearance,respiration,weight loss,total juice content,total soluble solids,and titratable acidity were registered during the study.The results indicate that the 1-MCP and Q combination favored fruit postharvest quality conditions such as reducing respiration rate;maintaining fruit good appearance,turgidity,and brighter fruit chromaticity;fruits treated with chitosan and stored at 12℃,favored total soluble solids.On the other hand,Variables such as weight loss,juice content,acidity,phenols,flavonoids,antioxidant activity,and hue values were favored by a 12℃ storing temperature regardless of the treatment.In general,the use of modified atmospheres,senescence retardants,and refrigeration as postharvest methods,can increase Persian lime shelf life.
文摘The effects of 1-methylcyclopropene (1-MCP) on postharvest quality of Zizania latifolia during storage at 25˚C were investigated. The results pointed out that a postharvest application of 1-MCP maintained the good visual appearance of fresh Z. latifolia, inhibited browning, mildew and weight loss at the bottom of Z. latifolia, and there is no significant changes on L, a*, b* and ΔE during the whole storage period. In addition, 1-MCP treatment inhibited the respiratory intensity of Z. latifolia during the first three days of storage, but it was significantly higher than that of the control on the sixth day of storage. Compared with the control, 1-MCP treatment maintained relatively high SOD, CAT, APX activities and low PAL, POD, PLD, lipase and LOX activities, delayed the decline of AsA content, reduced the accumulation of O<sub>2</sub><sup>-</sup>, H<sub>2</sub>O<sub>2</sub> and MDA, and ultimately maintained the integrity of cell structure and delayed the senescence of Z. latifolia. In addition, positive effects of 1-MCP on maintaining the cell structure integrity were observed in this investigation throughout the storage period at 25˚C.
基金Supported by Cooperation Project of Zhejiang Academy of Agricultural Sciences and the People's Government of Jinyun County(JY20140002)
文摘[Objective]The paper was to normalize the application of fenaminosulf in single cropping Zizania latifolia. [Method]Effects of spraying periods,different concentrations and spraying times of fenaminosulf on yield and output value of single cropping Z. latifolia were studied.[Result]In some regions where single cropping Z. latifolia could be harvested twice per year,fenaminosulf significantly promoted formation of swollen gall,and advanced the harvest period for about 50 d. Application of 0.5 g/L（ a.i.） fenaminosulf at 150,160 and 175 d post transplanting promoted swollen gall formation and enhanced the yield of Z. latifolia.In normal single cropping Z. latifolia growing regions,once application of fenaminosulf obviously advanced harvest period and improved yield and benefit of Z. latifolia,but with the increasing frequency of use,yield and benefit of Z. latifolia decreased significantly.[Conclusion]It is recommended that 1.0 g/L（a.i.） fenaminosulf can be applied for just once at 40-60 d post transplanting of Z. latifolia.
