Qfever is a worldwide zoonosis and its agent is Coxiella burnetii （C. burnetii）. There are two forms of Q fever： acute and chronic. Acute Q fever is caused by primary infection with C. burnetii and its main clinica...Qfever is a worldwide zoonosis and its agent is Coxiella burnetii （C. burnetii）. There are two forms of Q fever： acute and chronic. Acute Q fever is caused by primary infection with C. burnetii and its main clinical features are high fever, granulomatous hepatitis and atypical pneumonia. Acute Q fever is extremely prone to develop chronic infection if it is improperly treated. Endocarditis is the main characteristic of chronic Q fever and it accounts for 3% to 5% of all cases of endocarditis.展开更多
为了建立同步检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体感染的多重PCR方法,根据GenBank上具有属间特异性的布鲁菌.Bp26基因、鹦鹉热衣原体23S rRNA基因和贝纳氏柯克斯氏体IS1111a基因,利用Primer premier 5.0软件各设计1对特异...为了建立同步检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体感染的多重PCR方法,根据GenBank上具有属间特异性的布鲁菌.Bp26基因、鹦鹉热衣原体23S rRNA基因和贝纳氏柯克斯氏体IS1111a基因,利用Primer premier 5.0软件各设计1对特异性引物。通过优化PCR反应体系和扩增条件,建立了能够同时检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体的多重PCR方法。该方法具有较好的特异性和可重复性,对3种基因单重PCR检测敏感性均达到3.1×10~2拷贝·反应^-1,对3种病原基因同步检测的敏感性能达到3.1×10~3拷贝·反应^-1。利用该方法对采自不同流产牛的抗凝全血、血清、流产胎儿及奶液等172份临床样品进行检测,检测到布鲁菌感染阳性样品53份,鹦鹉热衣原体感染阳性样品2份,贝纳氏柯克斯氏体感染阳性样品10份,且检测到布鲁菌和鹦鹉衣原体混合感染阳性样品2份,布鲁菌和贝纳氏柯克斯氏体混合感染阳性样品2份,尚未检测到3种病原混合感染的阳性样品。结果表明,建立的多重PCR方法可以用来对奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体进行同步、快速、灵敏的检测。展开更多
文摘Qfever is a worldwide zoonosis and its agent is Coxiella burnetii （C. burnetii）. There are two forms of Q fever： acute and chronic. Acute Q fever is caused by primary infection with C. burnetii and its main clinical features are high fever, granulomatous hepatitis and atypical pneumonia. Acute Q fever is extremely prone to develop chronic infection if it is improperly treated. Endocarditis is the main characteristic of chronic Q fever and it accounts for 3% to 5% of all cases of endocarditis.
文摘为了建立同步检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体感染的多重PCR方法,根据GenBank上具有属间特异性的布鲁菌.Bp26基因、鹦鹉热衣原体23S rRNA基因和贝纳氏柯克斯氏体IS1111a基因,利用Primer premier 5.0软件各设计1对特异性引物。通过优化PCR反应体系和扩增条件,建立了能够同时检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体的多重PCR方法。该方法具有较好的特异性和可重复性,对3种基因单重PCR检测敏感性均达到3.1×10~2拷贝·反应^-1,对3种病原基因同步检测的敏感性能达到3.1×10~3拷贝·反应^-1。利用该方法对采自不同流产牛的抗凝全血、血清、流产胎儿及奶液等172份临床样品进行检测,检测到布鲁菌感染阳性样品53份,鹦鹉热衣原体感染阳性样品2份,贝纳氏柯克斯氏体感染阳性样品10份,且检测到布鲁菌和鹦鹉衣原体混合感染阳性样品2份,布鲁菌和贝纳氏柯克斯氏体混合感染阳性样品2份,尚未检测到3种病原混合感染的阳性样品。结果表明,建立的多重PCR方法可以用来对奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体进行同步、快速、灵敏的检测。
