As a staple food crop for one-third of the world＇s population, common wheat （Triticum aestivum L., 2n = 6x = 42, AABBDD） plays an important role in humans＇ food security. However, the genetic variation of cultivat...As a staple food crop for one-third of the world＇s population, common wheat （Triticum aestivum L., 2n = 6x = 42, AABBDD） plays an important role in humans＇ food security. However, the genetic variation of cultivated wheat has been dramatically narrowed by genetic erosion under the modem cultivation system, resulting in vulnerability to biotic and abiotic stresses （Jiang et al., 1994; Friebe et al., 1996）. The wild relatives of wheat represent a large reservoir of superior genes, and transferring these alien genes to modem cultivars through chromosome engineering is a successful method of broadening the genetic diversity of wheat （Chen et al., 2003;展开更多
The Traditional Chinese Medicine (TCM) ConstitutionClassification and the Constitutions in Chinese MedicineQuestionnaire (CCMQ) are based on nearly 30 years' researchon physique structure,physiological functions,p...The Traditional Chinese Medicine (TCM) ConstitutionClassification and the Constitutions in Chinese MedicineQuestionnaire (CCMQ) are based on nearly 30 years' researchon physique structure,physiological functions,psychologicalcharacteristics and reactive states.Epidemiology,immunology,molecular biology,genetics,mathematical statistics and multicrosseddisciplines were applied and many arguments were putforward by TCM experts,epidemiological specialists and constitutionexperts to establish the standardization tool Classificationand Diagnosis Standards for the Constitutions of TCM.At thesame time,basic research on constitution classification wasconducted to supply the objective basis of the classification standards.The Standards were used to conduct 21,948 cases ofepidemiological investigation on a national scale and showedgreat applicability,practicability and maneuverability.TheStandards were applied abroad in the medical services of TCMand were also an effective tool in the development of preventivetreatment of diseases by TCM.展开更多
Recombinant adeno-associated virus （rAAV） serotype 2, 3 and 8 vectors are the most promising liver- tropic AAV serotype vectors. Liver diseases are significant problems in China. However, to date, few studies on AAV...Recombinant adeno-associated virus （rAAV） serotype 2, 3 and 8 vectors are the most promising liver- tropic AAV serotype vectors. Liver diseases are significant problems in China. However, to date, few studies on AAV neutralizing antibodies （Nabs） were working with the Chinese population or with the rAAV3 vectors. The present study aimed to determine the prevalence of Nabs in Chinese population against wild-type AAV2, AAV3 and AAV8 capsids as well as additional two AAV3 variants. In addition, we performed a preliminary analysis to investigate the potential influence of traditional Chinese medicine body constitutions on AAV Nabs. Our work demonstrated that the majority of healthy Chinese subjects were positive for AAV Nabs, with the order of AAV2 〉 AAV3 = AAVLK03 〉 AAV8. There was no difference between： 1）AAV3 and its variants; 2） male and female subjects; and 3） different age cohorts （〈 35, 36- 50, and 〉 51 years old）. People in the Qi-deficiency constitution had significantly increased AAV8 Nabs than people in the Gentleness constitution. Our studies may have impact on the future clinical design of AAV-based gene therapy in the Chinese population.展开更多
基金supported by a grant from the National High Technology Research and Development Program("863" Program) of China(No. 2011AA100103)
文摘As a staple food crop for one-third of the world＇s population, common wheat （Triticum aestivum L., 2n = 6x = 42, AABBDD） plays an important role in humans＇ food security. However, the genetic variation of cultivated wheat has been dramatically narrowed by genetic erosion under the modem cultivation system, resulting in vulnerability to biotic and abiotic stresses （Jiang et al., 1994; Friebe et al., 1996）. The wild relatives of wheat represent a large reservoir of superior genes, and transferring these alien genes to modem cultivars through chromosome engineering is a successful method of broadening the genetic diversity of wheat （Chen et al., 2003;
基金Supported by National Basic Research Program of China (No.2005CB523501)
文摘The Traditional Chinese Medicine (TCM) ConstitutionClassification and the Constitutions in Chinese MedicineQuestionnaire (CCMQ) are based on nearly 30 years' researchon physique structure,physiological functions,psychologicalcharacteristics and reactive states.Epidemiology,immunology,molecular biology,genetics,mathematical statistics and multicrosseddisciplines were applied and many arguments were putforward by TCM experts,epidemiological specialists and constitutionexperts to establish the standardization tool Classificationand Diagnosis Standards for the Constitutions of TCM.At thesame time,basic research on constitution classification wasconducted to supply the objective basis of the classification standards.The Standards were used to conduct 21,948 cases ofepidemiological investigation on a national scale and showedgreat applicability,practicability and maneuverability.TheStandards were applied abroad in the medical services of TCMand were also an effective tool in the development of preventivetreatment of diseases by TCM.
文摘Recombinant adeno-associated virus （rAAV） serotype 2, 3 and 8 vectors are the most promising liver- tropic AAV serotype vectors. Liver diseases are significant problems in China. However, to date, few studies on AAV neutralizing antibodies （Nabs） were working with the Chinese population or with the rAAV3 vectors. The present study aimed to determine the prevalence of Nabs in Chinese population against wild-type AAV2, AAV3 and AAV8 capsids as well as additional two AAV3 variants. In addition, we performed a preliminary analysis to investigate the potential influence of traditional Chinese medicine body constitutions on AAV Nabs. Our work demonstrated that the majority of healthy Chinese subjects were positive for AAV Nabs, with the order of AAV2 〉 AAV3 = AAVLK03 〉 AAV8. There was no difference between： 1）AAV3 and its variants; 2） male and female subjects; and 3） different age cohorts （〈 35, 36- 50, and 〉 51 years old）. People in the Qi-deficiency constitution had significantly increased AAV8 Nabs than people in the Gentleness constitution. Our studies may have impact on the future clinical design of AAV-based gene therapy in the Chinese population.
