The migratory history of the engraulid fish Coilia nasus in the Rokkaku and Chikugo River estuaries of the Ariake Sea, Japan was assessed using otolith strontium（Sr） X-ray intensity maps and strontium：calcium（Sr...The migratory history of the engraulid fish Coilia nasus in the Rokkaku and Chikugo River estuaries of the Ariake Sea, Japan was assessed using otolith strontium（Sr） X-ray intensity maps and strontium：calcium（Sr：Ca） ratio life history transects by an electron probe microanalyzer（EPMA）. The results showed that seven of the ten specimens from the Rokkaku River Estuary（LJC） and all 15 specimens collected in the Chikugo River Estuary（ZHC） had low Sr：Ca ratios（≤3） at the central otolith area, indicating their riverine origin and initial freshwater residence. After the first regime shift adjacent to natal regions, the Sr level mapping displayed a wide variety of color patterns, and the Sr：Ca ratios obtained by the line transect analysis could be divided into one to six significantly different phases indicative of gradual life history transition. The other three specimens from the Rokkaku River Estuary had high Sr：Ca ratios（3–6.7） at the central otolith area but showed alternating changes between low and high values outside the natal region, suggesting that estuarine-origin individuals occurred in the Rokkaku River Estuary. The two-dimensional maps of the Sr level and average of the otolith Sr：Ca ratios along the life-history transects could be used as effective tools for reconstruction of past habitat use of the tapertail anchovy in estuaries of the Ariake Sea, Japan.展开更多
Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substituti...Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substitutions. At the third positions of genetic condon of cytochrome b gene, the two species show an extreme anti-G bias (<4%) and a pronounced bias towards A and C (>68%). There is no amino acid sequence divergence between the partial cytochrome b genes of the two species, indicating a close genetic relationship between them. The k-2p genetic distance of partial cytochrome b segment of the two species is 0.072, suggesting that the species were separated 3.6 Ma ago, in the middle Pliocene. Our result reveals that the cytochrome b gene is an appropriate marker for studies of population genetic structures and phylogeographic pat- terns of the two species.展开更多
Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths us...Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths using electron probe microanalysis. Individuals from the Oujiang River had higher and varied Sr:Ca ratios(expressed as(Sr:Ca)×1 000, 3.83–13.0 average) in the otolith core regions, suggesting that they were born in brackish or sea waters, and that a freshwater habitat might not be necessary for egg hatching and larval growth.While, individuals from the Zhujiang River had lower Sr:Ca ratios(0.39–2.51 average) in the core regions,suggesting a freshwater origin. After hatching, anchovies from the Zhujiang River migrate downstream to the river estuary close to brackish water. Our results demonstrated varied habitat use for spawning during stages of early life history between the two populations, and suggested that such variations are promoting diversity of life history strategies of this species.展开更多
Anchovies are migratory fish and adult individuals migrate in group from the sea into the river every year because of reproductive needs. TheYangtze River is the largest river in China, with rich food and fish species...Anchovies are migratory fish and adult individuals migrate in group from the sea into the river every year because of reproductive needs. TheYangtze River is the largest river in China, with rich food and fish species, hence, the Yangtze River would be an ideal habitat for growth and reproduction of anchovy. In this pa- per, based on fishing log data of anchovy in Jiangsu section of the Yangtze River, realted analysis was conducted in digital map using statistical software and GIS. From 2008 to 2011, the number of anchovy fishing vessels and fishing days in Jiangsu section remained stable, but the fishing volume and economic profit of single-vessel fluctuated constantly. This study provides references and advices for plan and management of anchovy fishery resource to administrative department.展开更多
The estuarine tapertail anchovy(Coilia nasus)is a high-value commercial fish.Estimating the spawning site or hatchery origin and habitat is essential for its conservation.This study aimed to determine the habitat use ...The estuarine tapertail anchovy(Coilia nasus)is a high-value commercial fish.Estimating the spawning site or hatchery origin and habitat is essential for its conservation.This study aimed to determine the habitat use and life history characteristics of C.nasus from the Changjiang River Estuary.We investigated the environmental signatures of strontium(Sr)and calcium(Ca)in the otoliths of the collected specimens using electron probe microanalysis;additionally,we examined their gonadal maturity stage.Our results indicate that the 31 adult C.nasus specimens used in this study could be classified into two types based on their otolith Sr:Ca concentration ratios and their gonadal maturity stage.The long freshwater early life history type(Type LF)had wider central region in the otolith with low Sr:Ca concentration ratios ranging from 1.24±0.62 to 1.92±0.78 and a bluish pattern of low Sr content level.These fish are of riverine origin and had a relatively long early life history in freshwater and low gonadal maturation when captured.The short freshwater early life history type(Type SF)had a shorter central region in the otolith with low Sr:Ca concentration ratios ranging from 1.35±0.5 to 2.82±0.97 and a correspondingly bluish pattern.These fish also had a relatively short-term early life history in freshwater and high gonadal maturation when captured.The results of the otolith microchemical analysis indicated that Type LF and Type SF originated in spawning/hatching sites far from and close to the estuary,respectively.The mature gonads of Type SF fish indicated that they may breed in areas close to the estuary,whereas the immature gonads of Type LF fish indicated that they may breed in areas far from the estuary.This study is the first to reveal that the Changjiang River Estuary contains stocks of anadromous C.nasus originating in different spawning sites during the same season.The estuarine habitat plays a critical role in the connectivity between freshwater recruitment and the marine resources available to adult spaw展开更多
Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic potential.However,the species faces c...Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic potential.However,the species faces challenges due to significant variation in the gonadal development rate among females,resulting in inconsistent ovarian maturation times at the population level,an extended reproductive period,and limitations on fish growth rate due to ovarian prematurity.In the present study,we combined genome-wide association study(GWAS)and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms(SNPs)and candidate genes associated with population-asynchronous ovarian development in C.nasus.Genotyping of the female population based on whole-genome resequencing yielded 2120695 high-quality SNPs,39 of which were suggestively associated with ovarian development.Of note,a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified,with cpne5a determined as the causal gene of the peak.Therefore,single-marker and haplotype association analyses were performed on cpne5a,revealing four genetic markers(P<0.05)and seven haplotypes(r2>0.9)significantly associated with the phenotype.Comparative transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary,respectively,between individuals of different body sizes.Integrating the GWAS and transcriptome analysis results,this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion,extracellular matrix,angiogenesis,and gap junctions involved in population-asynchronous ovarian development.The insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of C.nasus strains exhibiting population-synchronous ovarian development in the future.展开更多
The taxonomy of Coilia has been extensively studied in China,and yet phylogenetic relationships among component taxa remain controversial.We used a PCR-based fingerprinting technique,amplified fragment length polymorp...The taxonomy of Coilia has been extensively studied in China,and yet phylogenetic relationships among component taxa remain controversial.We used a PCR-based fingerprinting technique,amplified fragment length polymorphism(AFLP) to characterize and identify all four species of Coilia in China.We examined the genetic relationships of the four species of Coilia and a subspecies of Coilia nasus with AFLP.A total of 180 AFLP loci were generated from six primer combinations,of which 76.11% were polymorphic.The mean genetic distance between pairs of taxa ranged from 0.047 to 0.596.The neighbor-joining tree and UPGMA dendrogram resolved the investigated species into three separate lineages:(1) C.mystus,(2) C.grayii and(3) C.brachygnathus,C.nasus,and C.nasus taihuensis.Phylogenetic analysis of the AFLP data is inconsistent with current morphological taxonomic systems.The AFLP data indicated a close relationship among C.brachygnathus,C.nasus taihuensis,and C.nasus.Therefore,the two species described under Coilia(C.brachygnathus and C.nasus taihuensis) are treated as synonyms of C.nasus.展开更多
The Japanese grenadier anchovy (Coilia nasus) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may ...The Japanese grenadier anchovy (Coilia nasus) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may utilize olfactory cues to locate spawning grounds. We firstly performed a comparative proteome analysis about olfactory rosettes in two populations to hunt for the proteomic changes. Among 5 408 identified proteins, 1 515 proteins (629 up-regulated and 886 down-regulated) were differentially expressed. Especially, several proteins and pathways associated with olfactory signaling were found to be significantly differential. Compared with resident C. nasus, the expressions of Golf protein and the sodium/calcium exchanger were significantly up-regulated in anadromous C. nasus. The expression of adenylate cyclase and regulator of G-protein signaling (RGS) were decreased. Our findings suggest a decrease in the expression of cGMP-dependent protein kinase (PKG) in anadromous C. nasus compared to resident C. nasus. The expression of Calmodulin (CaM) was increased and CaM-dependent protein kinase Ⅱ(CaMKⅡ) was decreased. In addition, KEGG pathway enrichment analysis of up-regulated proteins indicated statistically significant difference not only in olfactory transduction but also in the cGMP-PKG signal pathway. Furtherly, we sought out some proteins expressed in the same trend occurring in DEGs (differentially expressed genes) and DEPs (differentially expressed proteins) by doing the integrative analysis of proteome and transcriptome in olfactory rosettes of C. nasus.展开更多
Coilia nasus is an important economic migratory fish in China, and it is known as ＂Three Delicious Fish of the Yangtze River＂ with puffer fishes and reeves shad. However, because of water pollution, blocked migratio...Coilia nasus is an important economic migratory fish in China, and it is known as ＂Three Delicious Fish of the Yangtze River＂ with puffer fishes and reeves shad. However, because of water pollution, blocked migration and overfishing, wild resources of C. nasus have suffered severe damage. This article gives an overview on genetic diversity of C. nasus from morphology, protein and DNA levels, to supply a theoretical foundation for protection of C. nasus resources.展开更多
Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role ...Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role in germ cells development.In this study,the cDNA sequence of boule(Cnbol)and dazl(Cndazl)genes were cloned and their expression patterns were characterized in Coilia nasus.RT-PCR showed that the expression of Cnbol and Cndazl mRNAs was restricted in adult gonads.The section in situ hybridization indicated that the temporospatial expression patterns of Cnbol and Cndazl were significantly different.In the testis,Cnbol was mainly detected in spermatocytes and spermatids,while Cndazl was prominently expressed in s permatogonia.In the ovary,Cnbol and Cndazl were highly expressed in the early stages of oocytes.Interestingly,Cndazl was also concentrated in perinuclear speckle and then localized to the Balbiani body in late stages of oocytes.In addition,Cnbol and Cndazl 3′UTR can specifically label medaka Primordial Germ Cells(PGCs).According to our results,Cnbol and Cndazl are marker genes of germ cells and may play a vital role in the development and differentiation of germ cells in Coilia nasus.展开更多
Spatial-temporal distribution of marine fishes is strongly influenced by environmental factors.To obtain a more continuous distribution of these variables usually measured by stationary sampling designs,spatial interp...Spatial-temporal distribution of marine fishes is strongly influenced by environmental factors.To obtain a more continuous distribution of these variables usually measured by stationary sampling designs,spatial interpolation methods(SIMs)is usually used.However,different SIMs may obtain varied estimation values with significant differences,thus affecting the prediction of fish spatial distribution.In this study,different SIMs were used to obtain continuous environmental variables(water depth,water temperature,salinity,dissolved oxygen(DO),p H,chlorophyll a and chemical oxygen demand(COD))in the Changjiang River Estuary(CRE),including inverse distance weighted(IDW)interpolation,ordinary Kriging(OK)(semivariogram model:exponential(OKE),Gaussian(OKG)and spherical(OKS))and radial basis function(RBF)(regularized spline function(RS)and tension spline function(TS)).The accuracy and effect of SIMs were cross-validated,and two-stage generalized additive model(GAM)was used to predict the distribution of Coilia nasus from 2012 to 2014 in CRE.DO and COD were removed before model prediction due to their autocorrelation coefficient based on variance inflation factors analysis.Results showed that the estimated values of environmental variables obtained by the different SIMs differed(i.e.,mean values,range etc.).Cross-validation revealed that the most suitable SIMs of water depth and chlorophyll a was IDW,water temperature and salinity was RS,and p H was OKG.Further,different interpolation results affected the predicted spatial distribution of Coilia nasus in the CRE.The mean values of the predicted abundance were similar,but the differences between and among the maximum value were large.Studies showed that different SIMs can affect estimated values of the environmental variables in the CRE(especially salinity).These variations further suggest that the most applicable SIMs to each variable will also differ.Thus,it is necessary to take these potential impacts into consideration when studying the relationship between the spatial d展开更多
DEAR EDITOR,Anadromous Coilia nasus is a socioeconomically important species from the middle and lower reaches of the Yangtze River,China.Here,we compared growth and feeding of C.nasus larvae in four reaches along the...DEAR EDITOR,Anadromous Coilia nasus is a socioeconomically important species from the middle and lower reaches of the Yangtze River,China.Here,we compared growth and feeding of C.nasus larvae in four reaches along the full migration corridor of the river(640 km)to determine how fitness varies between progenies of short-and long-distance migrants.Results demonstrated that larvae collected in downstream sections grew faster,exhibited higher feeding intensities.展开更多
This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both...This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both seawater and freshwater except for gut which was only from the fish conditioned in freshwater,were isolated.All the RNAs were pooled together equally for SMRT sequencing.One SMRT cell produced 64.18 Gb clean data,including 765,184 circular consensus(CCS)reads and 616,126 full-length non-chimeric(FLNC)reads.Finally,93,793 non-redundant transcripts were obtained after clustered and polished.Structural analysis of the 93,793 non-redundant transcripts predicted 8242 alternative splicing events,a total of 229,825 SSR and 72,037 complete CDS.A total of 8437 lncRNA were identified.84,617 transcripts were annotated in NR,Swissprot,GO,COG,KOG,eggnog,Pfam and KEGG database totally.This is the first full-length transcriptome of Coilia nasus,including transcripts appeared in the case of seawater,which will facilitate exploration of genetic data and life story of Coilia nasus.展开更多
Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each in...Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each individual, and Burnaby＇s multivariate method was used to obtain size-adjusted shape data. The cluster analysis and discriminant analysis were used to discriminate morphological differences among populations. The results indicated that 1） the three populations were clustered into two distinct groups： the first group included Changiiang C. mystus and Zhujiang C. mystus, the last one included Minjiang C. mystus, and 2） discriminant analysis with selected 5 morphological parameters showed that the identification accuracy was between 98.7952% and 100%, and global identification accuracy was 99.2933%. Reproductive isolation and adaption to environmental condition are determinant factors for morphological variation between populations of C. mystus.展开更多
基金The National Natural Science Foundation of China under contract No.31372533China Central Governmental Research Institutional Basic Special Research Project from Public Welfare Fund under contract No.2016PT01the“948”Program of the Ministry of Agriculture under contract No.2014-S6
文摘The migratory history of the engraulid fish Coilia nasus in the Rokkaku and Chikugo River estuaries of the Ariake Sea, Japan was assessed using otolith strontium（Sr） X-ray intensity maps and strontium：calcium（Sr：Ca） ratio life history transects by an electron probe microanalyzer（EPMA）. The results showed that seven of the ten specimens from the Rokkaku River Estuary（LJC） and all 15 specimens collected in the Chikugo River Estuary（ZHC） had low Sr：Ca ratios（≤3） at the central otolith area, indicating their riverine origin and initial freshwater residence. After the first regime shift adjacent to natal regions, the Sr level mapping displayed a wide variety of color patterns, and the Sr：Ca ratios obtained by the line transect analysis could be divided into one to six significantly different phases indicative of gradual life history transition. The other three specimens from the Rokkaku River Estuary had high Sr：Ca ratios（3–6.7） at the central otolith area but showed alternating changes between low and high values outside the natal region, suggesting that estuarine-origin individuals occurred in the Rokkaku River Estuary. The two-dimensional maps of the Sr level and average of the otolith Sr：Ca ratios along the life-history transects could be used as effective tools for reconstruction of past habitat use of the tapertail anchovy in estuaries of the Ariake Sea, Japan.
基金the Major State Basic Research Development Program(G19990437).
文摘Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substitutions. At the third positions of genetic condon of cytochrome b gene, the two species show an extreme anti-G bias (<4%) and a pronounced bias towards A and C (>68%). There is no amino acid sequence divergence between the partial cytochrome b genes of the two species, indicating a close genetic relationship between them. The k-2p genetic distance of partial cytochrome b segment of the two species is 0.072, suggesting that the species were separated 3.6 Ma ago, in the middle Pliocene. Our result reveals that the cytochrome b gene is an appropriate marker for studies of population genetic structures and phylogeographic pat- terns of the two species.
基金The China Central Governmental Research Institutional Basic Special Research Project from Public Welfare Fund under contract No.2016PT01the Open Project for Key Laboratory of South China Sea Fishery Research Exploitation&Utilization of Ministry of Agriculture under contract No.FREU2015-07+2 种基金the Central Public-interest Scientific Institution Based Research Fund,Freshwater Fisheries Research Center,CAFS under contract No.2017JBFR02the National Natural Science Foundation of China under contract No.31602159the Natural Science Foundation of Jiangsu Province,China under contract No.BK20160204
文摘Habitat use of the tapertail anchovy(Coilia mystus Linnaeus, 1758) from the Oujiang River Estuary and the Zhujiang(Pearl) River Estuary was studied by examining the environmental signatures of Sr and Ca in otoliths using electron probe microanalysis. Individuals from the Oujiang River had higher and varied Sr:Ca ratios(expressed as(Sr:Ca)×1 000, 3.83–13.0 average) in the otolith core regions, suggesting that they were born in brackish or sea waters, and that a freshwater habitat might not be necessary for egg hatching and larval growth.While, individuals from the Zhujiang River had lower Sr:Ca ratios(0.39–2.51 average) in the core regions,suggesting a freshwater origin. After hatching, anchovies from the Zhujiang River migrate downstream to the river estuary close to brackish water. Our results demonstrated varied habitat use for spawning during stages of early life history between the two populations, and suggested that such variations are promoting diversity of life history strategies of this species.
基金Supported by the R&D Special Fund for Public Welfare Industry(201203086)Special Financial Fund of Ministry of Agriculture(6125005)Central Public-interest Scientific Institution Basal Research Fund(2011JBFC04)~~
文摘Anchovies are migratory fish and adult individuals migrate in group from the sea into the river every year because of reproductive needs. TheYangtze River is the largest river in China, with rich food and fish species, hence, the Yangtze River would be an ideal habitat for growth and reproduction of anchovy. In this pa- per, based on fishing log data of anchovy in Jiangsu section of the Yangtze River, realted analysis was conducted in digital map using statistical software and GIS. From 2008 to 2011, the number of anchovy fishing vessels and fishing days in Jiangsu section remained stable, but the fishing volume and economic profit of single-vessel fluctuated constantly. This study provides references and advices for plan and management of anchovy fishery resource to administrative department.
基金The National Natural Science Foundation of China under contract No.31602159the Agricultural Finance Special Project under contract No.CJDC-2017-22the Scientific Research Foundation of Wuxi City for Returned Oversea Chinese Scholars under contract No.CZ2019231400.
文摘The estuarine tapertail anchovy(Coilia nasus)is a high-value commercial fish.Estimating the spawning site or hatchery origin and habitat is essential for its conservation.This study aimed to determine the habitat use and life history characteristics of C.nasus from the Changjiang River Estuary.We investigated the environmental signatures of strontium(Sr)and calcium(Ca)in the otoliths of the collected specimens using electron probe microanalysis;additionally,we examined their gonadal maturity stage.Our results indicate that the 31 adult C.nasus specimens used in this study could be classified into two types based on their otolith Sr:Ca concentration ratios and their gonadal maturity stage.The long freshwater early life history type(Type LF)had wider central region in the otolith with low Sr:Ca concentration ratios ranging from 1.24±0.62 to 1.92±0.78 and a bluish pattern of low Sr content level.These fish are of riverine origin and had a relatively long early life history in freshwater and low gonadal maturation when captured.The short freshwater early life history type(Type SF)had a shorter central region in the otolith with low Sr:Ca concentration ratios ranging from 1.35±0.5 to 2.82±0.97 and a correspondingly bluish pattern.These fish also had a relatively short-term early life history in freshwater and high gonadal maturation when captured.The results of the otolith microchemical analysis indicated that Type LF and Type SF originated in spawning/hatching sites far from and close to the estuary,respectively.The mature gonads of Type SF fish indicated that they may breed in areas close to the estuary,whereas the immature gonads of Type LF fish indicated that they may breed in areas far from the estuary.This study is the first to reveal that the Changjiang River Estuary contains stocks of anadromous C.nasus originating in different spawning sites during the same season.The estuarine habitat plays a critical role in the connectivity between freshwater recruitment and the marine resources available to adult spaw
基金supported by the National Key R&D Program of China(2022YFD2400904)Key R&D Projects in Hubei Province(2022BBA008)+1 种基金Zhenjiang Jinshan TalentsWuhan Yangtze River Characteristic Fish Breeding and Domestication Project。
文摘Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic potential.However,the species faces challenges due to significant variation in the gonadal development rate among females,resulting in inconsistent ovarian maturation times at the population level,an extended reproductive period,and limitations on fish growth rate due to ovarian prematurity.In the present study,we combined genome-wide association study(GWAS)and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms(SNPs)and candidate genes associated with population-asynchronous ovarian development in C.nasus.Genotyping of the female population based on whole-genome resequencing yielded 2120695 high-quality SNPs,39 of which were suggestively associated with ovarian development.Of note,a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified,with cpne5a determined as the causal gene of the peak.Therefore,single-marker and haplotype association analyses were performed on cpne5a,revealing four genetic markers(P<0.05)and seven haplotypes(r2>0.9)significantly associated with the phenotype.Comparative transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary,respectively,between individuals of different body sizes.Integrating the GWAS and transcriptome analysis results,this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion,extracellular matrix,angiogenesis,and gap junctions involved in population-asynchronous ovarian development.The insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of C.nasus strains exhibiting population-synchronous ovarian development in the future.
基金Supported by the Special Research Foundation for Public Welfare Marine Program(No.200905019-1)the National Natural Science Foundation of China(Nos.30770354,40676086)
文摘The taxonomy of Coilia has been extensively studied in China,and yet phylogenetic relationships among component taxa remain controversial.We used a PCR-based fingerprinting technique,amplified fragment length polymorphism(AFLP) to characterize and identify all four species of Coilia in China.We examined the genetic relationships of the four species of Coilia and a subspecies of Coilia nasus with AFLP.A total of 180 AFLP loci were generated from six primer combinations,of which 76.11% were polymorphic.The mean genetic distance between pairs of taxa ranged from 0.047 to 0.596.The neighbor-joining tree and UPGMA dendrogram resolved the investigated species into three separate lineages:(1) C.mystus,(2) C.grayii and(3) C.brachygnathus,C.nasus,and C.nasus taihuensis.Phylogenetic analysis of the AFLP data is inconsistent with current morphological taxonomic systems.The AFLP data indicated a close relationship among C.brachygnathus,C.nasus taihuensis,and C.nasus.Therefore,the two species described under Coilia(C.brachygnathus and C.nasus taihuensis) are treated as synonyms of C.nasus.
基金Supported by National Key R&D Program of China(No.2018YFD0900800)the National Natural Science Foundation of China(Nos.31472280,31172407)
文摘The Japanese grenadier anchovy (Coilia nasus) undergoes upstream migration to spawning annually but can also be observed in freshwater resident populations. It has been hypothesized that anadromous adult C. nasus may utilize olfactory cues to locate spawning grounds. We firstly performed a comparative proteome analysis about olfactory rosettes in two populations to hunt for the proteomic changes. Among 5 408 identified proteins, 1 515 proteins (629 up-regulated and 886 down-regulated) were differentially expressed. Especially, several proteins and pathways associated with olfactory signaling were found to be significantly differential. Compared with resident C. nasus, the expressions of Golf protein and the sodium/calcium exchanger were significantly up-regulated in anadromous C. nasus. The expression of adenylate cyclase and regulator of G-protein signaling (RGS) were decreased. Our findings suggest a decrease in the expression of cGMP-dependent protein kinase (PKG) in anadromous C. nasus compared to resident C. nasus. The expression of Calmodulin (CaM) was increased and CaM-dependent protein kinase Ⅱ(CaMKⅡ) was decreased. In addition, KEGG pathway enrichment analysis of up-regulated proteins indicated statistically significant difference not only in olfactory transduction but also in the cGMP-PKG signal pathway. Furtherly, we sought out some proteins expressed in the same trend occurring in DEGs (differentially expressed genes) and DEPs (differentially expressed proteins) by doing the integrative analysis of proteome and transcriptome in olfactory rosettes of C. nasus.
文摘Coilia nasus is an important economic migratory fish in China, and it is known as ＂Three Delicious Fish of the Yangtze River＂ with puffer fishes and reeves shad. However, because of water pollution, blocked migration and overfishing, wild resources of C. nasus have suffered severe damage. This article gives an overview on genetic diversity of C. nasus from morphology, protein and DNA levels, to supply a theoretical foundation for protection of C. nasus resources.
基金supported by the National Key R&D Program of China(2018YFD0901205)National Natural Science Foundation of China(31672700).
文摘Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role in germ cells development.In this study,the cDNA sequence of boule(Cnbol)and dazl(Cndazl)genes were cloned and their expression patterns were characterized in Coilia nasus.RT-PCR showed that the expression of Cnbol and Cndazl mRNAs was restricted in adult gonads.The section in situ hybridization indicated that the temporospatial expression patterns of Cnbol and Cndazl were significantly different.In the testis,Cnbol was mainly detected in spermatocytes and spermatids,while Cndazl was prominently expressed in s permatogonia.In the ovary,Cnbol and Cndazl were highly expressed in the early stages of oocytes.Interestingly,Cndazl was also concentrated in perinuclear speckle and then localized to the Balbiani body in late stages of oocytes.In addition,Cnbol and Cndazl 3′UTR can specifically label medaka Primordial Germ Cells(PGCs).According to our results,Cnbol and Cndazl are marker genes of germ cells and may play a vital role in the development and differentiation of germ cells in Coilia nasus.
基金The Shanghai Municipal Science and Technology Commission Local Capacity Construction Project under contract No.18050502000the Monitoring and Evaluation of National Sea Ranch Demonstration Area Project in Changjiang River Estuary under contract No.171015the National Natural Science Foundation of China under contract No.41906074。
文摘Spatial-temporal distribution of marine fishes is strongly influenced by environmental factors.To obtain a more continuous distribution of these variables usually measured by stationary sampling designs,spatial interpolation methods(SIMs)is usually used.However,different SIMs may obtain varied estimation values with significant differences,thus affecting the prediction of fish spatial distribution.In this study,different SIMs were used to obtain continuous environmental variables(water depth,water temperature,salinity,dissolved oxygen(DO),p H,chlorophyll a and chemical oxygen demand(COD))in the Changjiang River Estuary(CRE),including inverse distance weighted(IDW)interpolation,ordinary Kriging(OK)(semivariogram model:exponential(OKE),Gaussian(OKG)and spherical(OKS))and radial basis function(RBF)(regularized spline function(RS)and tension spline function(TS)).The accuracy and effect of SIMs were cross-validated,and two-stage generalized additive model(GAM)was used to predict the distribution of Coilia nasus from 2012 to 2014 in CRE.DO and COD were removed before model prediction due to their autocorrelation coefficient based on variance inflation factors analysis.Results showed that the estimated values of environmental variables obtained by the different SIMs differed(i.e.,mean values,range etc.).Cross-validation revealed that the most suitable SIMs of water depth and chlorophyll a was IDW,water temperature and salinity was RS,and p H was OKG.Further,different interpolation results affected the predicted spatial distribution of Coilia nasus in the CRE.The mean values of the predicted abundance were similar,but the differences between and among the maximum value were large.Studies showed that different SIMs can affect estimated values of the environmental variables in the CRE(especially salinity).These variations further suggest that the most applicable SIMs to each variable will also differ.Thus,it is necessary to take these potential impacts into consideration when studying the relationship between the spatial d
基金supported by the National Natural Science Foundation of China(32002394,31570420)supported by the Peter B.Moyle&California Trout Endowment for Coldwater Fish Conservationthe California Agricultural Experimental Station of the University of California Davis(CA-D-WFB-2467-H)。
文摘DEAR EDITOR,Anadromous Coilia nasus is a socioeconomically important species from the middle and lower reaches of the Yangtze River,China.Here,we compared growth and feeding of C.nasus larvae in four reaches along the full migration corridor of the river(640 km)to determine how fitness varies between progenies of short-and long-distance migrants.Results demonstrated that larvae collected in downstream sections grew faster,exhibited higher feeding intensities.
基金This work was supported by the Project of Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN)of the Ministry of Agriculture and Rural Affairs,Shanghai Ocean University(A1-3201-19-300204)the Project of Science and Technology Commission Shanghai Municipality(17391900300).
文摘This study was designed to generate the full-length transcriptome of Coilia nasus using single-molecule long read isoform sequencing(SMRT-seq)technology.RNAs of brains,ovaries,and testes of C.nasus conditioned in both seawater and freshwater except for gut which was only from the fish conditioned in freshwater,were isolated.All the RNAs were pooled together equally for SMRT sequencing.One SMRT cell produced 64.18 Gb clean data,including 765,184 circular consensus(CCS)reads and 616,126 full-length non-chimeric(FLNC)reads.Finally,93,793 non-redundant transcripts were obtained after clustered and polished.Structural analysis of the 93,793 non-redundant transcripts predicted 8242 alternative splicing events,a total of 229,825 SSR and 72,037 complete CDS.A total of 8437 lncRNA were identified.84,617 transcripts were annotated in NR,Swissprot,GO,COG,KOG,eggnog,Pfam and KEGG database totally.This is the first full-length transcriptome of Coilia nasus,including transcripts appeared in the case of seawater,which will facilitate exploration of genetic data and life story of Coilia nasus.
基金The author would like to thank Mrs. Jindi Han, Mr. Xiaoguo Li, Mr. Guomin He, Mr. Zhenran Chen, and Dr. Jixing Zou for assistance in sampling and data collection. This work was supported by the National Natural Science Foundation of China （No. 30600456）, Commission of Science and Technology of Shanghai, China （No. 08391910300）, Project of Keji Xing Nong of Shanghai, China （No. 2008-7-2）, open project of Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture （No. Shikai-2005-06）, and special research fund for the national non-profit institutes （East China Sea Fisheries Research Institute, Project No. 2008Z02）.
文摘Truss network data were collected and investigated in order to clarify the morphological variation in populations of Coilia mystus from three Chinese estuaries. Nineteen morphometric measurements were made for each individual, and Burnaby＇s multivariate method was used to obtain size-adjusted shape data. The cluster analysis and discriminant analysis were used to discriminate morphological differences among populations. The results indicated that 1） the three populations were clustered into two distinct groups： the first group included Changiiang C. mystus and Zhujiang C. mystus, the last one included Minjiang C. mystus, and 2） discriminant analysis with selected 5 morphological parameters showed that the identification accuracy was between 98.7952% and 100%, and global identification accuracy was 99.2933%. Reproductive isolation and adaption to environmental condition are determinant factors for morphological variation between populations of C. mystus.
