A rapid and accurate ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-MS/MS)method was established and validated for the measurement of two forms of cardamonin,i.e.,fr...A rapid and accurate ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-MS/MS)method was established and validated for the measurement of two forms of cardamonin,i.e.,free and glucuronidated,in the plasma and bile of rats.Cardamonin and an internal standard(1,8-dihydroxyanthraquinone)were extracted from plasma and bile with ethyl ether via liquid-liquid extraction.The analytes in the extracts were separated by an Agilent Zorbax Stable Bond-C18 column(2.1 mm×50 mm,1.8μm)under isocratic elution conditions[acetonitrile(A)and 0.1%ammonium formate in water(B),40:60(volume ratio)]with a flow rate of 0.4 mL/min,and mass spectrometry in negative ion MRM mode was implemented for analysis.Good linearity over the wide ranges of 0.01–5μg/mL for plasma and 0.025–10μg/mL for bile samples was acquired.Method validation was performed according to the FDA guidelines for bioanalytical methods.展开更多
文摘A rapid and accurate ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-MS/MS)method was established and validated for the measurement of two forms of cardamonin,i.e.,free and glucuronidated,in the plasma and bile of rats.Cardamonin and an internal standard(1,8-dihydroxyanthraquinone)were extracted from plasma and bile with ethyl ether via liquid-liquid extraction.The analytes in the extracts were separated by an Agilent Zorbax Stable Bond-C18 column(2.1 mm×50 mm,1.8μm)under isocratic elution conditions[acetonitrile(A)and 0.1%ammonium formate in water(B),40:60(volume ratio)]with a flow rate of 0.4 mL/min,and mass spectrometry in negative ion MRM mode was implemented for analysis.Good linearity over the wide ranges of 0.01–5μg/mL for plasma and 0.025–10μg/mL for bile samples was acquired.Method validation was performed according to the FDA guidelines for bioanalytical methods.
