AIM:To analyze the hepatitis B virus(HBV) characters in China,as well as the correlation between several HBV mutation and hepatitis symptoms.METHODS:A total of 1148 HBV genome sequences from patients throughout China ...AIM:To analyze the hepatitis B virus(HBV) characters in China,as well as the correlation between several HBV mutation and hepatitis symptoms.METHODS:A total of 1148 HBV genome sequences from patients throughout China were collected via the National Center For Biotechnology Information database(information including:genotype,territory and clinical status).HBV genotypes were classified by a direct reference from the Genbank sequence annotation,phylogenetic tree and online software analysis(http://www.ncbi.nlm.nih.gov/projects/genotyping/formpage.cgi).The phylogenetic tree was constructed based on the neighbor-joining method by MEGA5.0 software.HBV sequences were grouped based on phylogenetic tree and the distance between the groups was calculated by using the computer between group mean distance methods.Seven hundred and twelve HBV sequences with clear annotation of clinical symptoms were selected to analyses the correlation of mutation and clinical symptoms.Characteristics of sequences were analyzed by using DNAStar and BioEdit software packages.The codon usage bias and RNA secondary structures analysis were performed by RNAdraw software.Recombination analysis was performed by using Simplot software.RESULTS:In China,HBV genotype C was the predominant in Northeastern,genotype B was predominant in Central Southern areas,genotype B and C were both dominant in Southwestern areas,and the recombinant genotype C/D was predominant in Northwestern areas.C2 and B2 were identified as the two major sub-genotypes,FJ386674 might be a putative sub-genotype as B10.The basal core promoter double mutation and pre-C mutation showed various significant differences between hepatitis symptoms.In addition to ATG,many other HBV initiation codons also exist.HBV has codon usage bias;the termination codon of X,C and P open reading frames(ORF) were TAA,TAG,and TGA,respectively.The major stop codons of S-ORF were TAA(96.45%) and TGA(83.60%) in B2 and C2 subtype,respectively.CONCLUSION:This study recapitulated the epidemiology of HBV in China,展开更多
目的探讨腰椎间盘突出症患者临床表现严重程度与CT影像学的相关性。方法选取2014年7月—2018年7月浙江衢化医院脊柱外科收治的167例腰椎间盘突出症患者,按照下腰痛评估量表的判定结果将其分为轻度(58例)、中度(64例)和重度(45例)。分析...目的探讨腰椎间盘突出症患者临床表现严重程度与CT影像学的相关性。方法选取2014年7月—2018年7月浙江衢化医院脊柱外科收治的167例腰椎间盘突出症患者,按照下腰痛评估量表的判定结果将其分为轻度(58例)、中度(64例)和重度(45例)。分析其严重程度与CT影像学指标的相关性,以及与硬膜囊前后径、矢状径的相关性,用Pearson相关性分析日本骨科协会制定的改良后下腰痛评分(modified Japanese orthopaedic association score,mJOA)与硬膜囊前后径、矢状径相关系数。结果腰椎间盘突出症轻、中、重度患者在髓核经骨突出情况、髓核突出后的病理类型、髓核密度值、髓核经额状面的突出程度、髓核经水平面的突出程度、髓核突出后与神经根的关系、髓核突出后对硬膜囊的压迫程度的差异具有统计学意义(P<0.05);轻、中、重度患者中,硬膜囊前后径依次增大(均P<0.05),矢状径依次减小(均P<0.05); mJOA评分与硬膜囊前后径呈正相关(r=0.584,P=0.017),与硬膜囊矢状径呈负相关(r=-0.603,P=0.025)。结论对于绝大多数腰椎间盘突出症患者,其临床表现的严重程度与部分CT影像学指标具有相关性。展开更多
基金Supported by National Natural Science Foundation of China,No.81160352grants from the Education Department Foundation of Yunnan Province,No.2012J091+1 种基金Health Bureau of Yunnan Province,No.D-201203(partly)Science and Technology Department of Yunnan Province,No.2013HB084(partly)
文摘AIM:To analyze the hepatitis B virus(HBV) characters in China,as well as the correlation between several HBV mutation and hepatitis symptoms.METHODS:A total of 1148 HBV genome sequences from patients throughout China were collected via the National Center For Biotechnology Information database(information including:genotype,territory and clinical status).HBV genotypes were classified by a direct reference from the Genbank sequence annotation,phylogenetic tree and online software analysis(http://www.ncbi.nlm.nih.gov/projects/genotyping/formpage.cgi).The phylogenetic tree was constructed based on the neighbor-joining method by MEGA5.0 software.HBV sequences were grouped based on phylogenetic tree and the distance between the groups was calculated by using the computer between group mean distance methods.Seven hundred and twelve HBV sequences with clear annotation of clinical symptoms were selected to analyses the correlation of mutation and clinical symptoms.Characteristics of sequences were analyzed by using DNAStar and BioEdit software packages.The codon usage bias and RNA secondary structures analysis were performed by RNAdraw software.Recombination analysis was performed by using Simplot software.RESULTS:In China,HBV genotype C was the predominant in Northeastern,genotype B was predominant in Central Southern areas,genotype B and C were both dominant in Southwestern areas,and the recombinant genotype C/D was predominant in Northwestern areas.C2 and B2 were identified as the two major sub-genotypes,FJ386674 might be a putative sub-genotype as B10.The basal core promoter double mutation and pre-C mutation showed various significant differences between hepatitis symptoms.In addition to ATG,many other HBV initiation codons also exist.HBV has codon usage bias;the termination codon of X,C and P open reading frames(ORF) were TAA,TAG,and TGA,respectively.The major stop codons of S-ORF were TAA(96.45%) and TGA(83.60%) in B2 and C2 subtype,respectively.CONCLUSION:This study recapitulated the epidemiology of HBV in China,
文摘目的探讨腰椎间盘突出症患者临床表现严重程度与CT影像学的相关性。方法选取2014年7月—2018年7月浙江衢化医院脊柱外科收治的167例腰椎间盘突出症患者,按照下腰痛评估量表的判定结果将其分为轻度(58例)、中度(64例)和重度(45例)。分析其严重程度与CT影像学指标的相关性,以及与硬膜囊前后径、矢状径的相关性,用Pearson相关性分析日本骨科协会制定的改良后下腰痛评分(modified Japanese orthopaedic association score,mJOA)与硬膜囊前后径、矢状径相关系数。结果腰椎间盘突出症轻、中、重度患者在髓核经骨突出情况、髓核突出后的病理类型、髓核密度值、髓核经额状面的突出程度、髓核经水平面的突出程度、髓核突出后与神经根的关系、髓核突出后对硬膜囊的压迫程度的差异具有统计学意义(P<0.05);轻、中、重度患者中,硬膜囊前后径依次增大(均P<0.05),矢状径依次减小(均P<0.05); mJOA评分与硬膜囊前后径呈正相关(r=0.584,P=0.017),与硬膜囊矢状径呈负相关(r=-0.603,P=0.025)。结论对于绝大多数腰椎间盘突出症患者,其临床表现的严重程度与部分CT影像学指标具有相关性。
