Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly....Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly. Furthermore we found that chromosomal telomere DNA could bind to nuclear matrix specifically by the dot and Southern hybridization. The result of the Southwestern blot suggests that telomere DNA has high affinity to lamin B, vimentin and some nuclear matrix proteins. Therefore, the nuclear matrix and lamina of HeLa cell are possibly associated with spatial organization and action of chromosome.展开更多
Telomeres,the natural termini of chromosomes in eukaryotes,play a very importantrole in stability and complete replication of chromosomes.Previous observations suggestthat chromosomes have non-random distribution in i...Telomeres,the natural termini of chromosomes in eukaryotes,play a very importantrole in stability and complete replication of chromosomes.Previous observations suggestthat chromosomes have non-random distribution in interphase nuclei,and their telomeresusually exist under nuclear envelope.However,no direct evidence available supportssuch an idea.In recent years telomeric DNA sequences of many organizations have beenfound based on the great progress of telomeric study.Therefore we can study展开更多
文摘Using Electron Spectroscopic Imaging (ESI), we visualized the in situ binding of nucleic acids to nuclear matrix and 3H-thymidine incorporation which indicates that a small partial DNA bound to nuclear matrix tightly. Furthermore we found that chromosomal telomere DNA could bind to nuclear matrix specifically by the dot and Southern hybridization. The result of the Southwestern blot suggests that telomere DNA has high affinity to lamin B, vimentin and some nuclear matrix proteins. Therefore, the nuclear matrix and lamina of HeLa cell are possibly associated with spatial organization and action of chromosome.
文摘Telomeres,the natural termini of chromosomes in eukaryotes,play a very importantrole in stability and complete replication of chromosomes.Previous observations suggestthat chromosomes have non-random distribution in interphase nuclei,and their telomeresusually exist under nuclear envelope.However,no direct evidence available supportssuch an idea.In recent years telomeric DNA sequences of many organizations have beenfound based on the great progress of telomeric study.Therefore we can study
