Chromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice ...Chromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome展开更多
Heterosis for yield and its component traits between chromosome segments from IR24, an indica variety, and the counterparts from 02428, a japonica rice, was inves- tigated by using a hybrid F1 population composed of 6...Heterosis for yield and its component traits between chromosome segments from IR24, an indica variety, and the counterparts from 02428, a japonica rice, was inves- tigated by using a hybrid F1 population composed of 63 com- binations between 02428 and IR24 chromosome segment substitution lines (CSSLs) with the genetic background of Asominori, a japonica variety. Significant differences in het- erosis for yield and yield-component traits were observed among the crosses. Analysis of graphical genotyping showed that 14 substituted segments were responsible for yield het- erosis. All of them were from all the 12 chromosomes of IR24 except chromosomes 8 and 10. Six segments at the intervals of RFLP markers, such as X132—G1340—R459, X48— C393A, R288—R1854, R2918—X52, X257—C1350 and R367 —X189-2—X24-2 on chromosomes 2, 3, 4, 11 and 12 respec- tively, had very significant heterosis for yield at the level of P ≤0.005 based on t-test, individually increasing the hybrid yield by more than 35% compared with the control cross “Asominori×02428”. Most of IR24 chromosome segments were found to have no significant hybrid effect for yield and yield-component traits, and one segment located at R2171 on chromosome 6 possessed significant negative effect with 27% of yield decrease. Advantages of using CSSLs in the heterosis studies were discussed and approaches of the partial and genome-wide exploitation of rice heterosis between indica and japonica by molecular marker-assisted selection were then proposed.展开更多
对陆海BC4F3和BC4F3:4代换系群体的纤维产量与品质的表型性状进行初步评价分析,结果表明,群体代换系各性状平均值与轮回亲本中棉所45相近,但群体内部个体间仍存在丰富的遗传变异,其中不乏超越中棉所45的材料。从中筛选20个纤维品质突出...对陆海BC4F3和BC4F3:4代换系群体的纤维产量与品质的表型性状进行初步评价分析,结果表明,群体代换系各性状平均值与轮回亲本中棉所45相近,但群体内部个体间仍存在丰富的遗传变异,其中不乏超越中棉所45的材料。从中筛选20个纤维品质突出单株(株行),2年的上半部平均长度高于30.00 mm,断裂比强度高于31.0 c N/tex,表现较好的一致性和稳定性,为进一步棉花纤维品质育种提供了选择材料。展开更多
以综合性状优良的烤烟种质Y3为轮回亲本,抗烟草黑胫病（0号和1号生理小种）和赤星病的雪茄烟种质Beinhart 1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Bein...以综合性状优良的烤烟种质Y3为轮回亲本,抗烟草黑胫病（0号和1号生理小种）和赤星病的雪茄烟种质Beinhart 1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Beinhart 1000-1和K326染色体片段株系代换系群体。该群体携带377个代换片段,分布于烟草24条连锁群上。每个株系携带1~5个代换片段,代换片段长度介于0.05~36.88 c M,平均长度7.75 c M。代换片段重叠累加总长度为2922.57 c M,是烟草基因组总长度的2.61倍。代换片段覆盖总长度为1114.32 c M,烟草基因组覆盖率为99.45%。本研究构建的片段代换系可用于烟草基因定位、复杂性状的QTL分析和标记辅助选择育种。展开更多
基金supported in part by the National Basic Research Program(Nos.2012CB944803 and 2011CB 100202)the National Natural Science Foundation(No.31071383)+1 种基金the National Special Program for Transgenic Research(No. 2009ZX08009-008B)the Priority Academic Program Development from Jiangsu Government of China
文摘Chromosome segment substitution lines（CSSLs） are useful for the precise mapping of quantitative trait loci（QTLs） and dissection of the genetic basis of complex traits.In this study,two whole-genome sequenced rice cultivars,the japonica Nipponbare and indica 9311 were used as recipient and donor,respectively.A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy.Detailed graphical genotypes of 38 lines were constructed based on resequencing data.These CSSLs had a total of 95 substituted segments derived from indica 9311,with an average of about 2.5 segments per CSSL and eight segments per chromosome,and covered about 87.4%of the rice whole genome.A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight.The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice.These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome
基金This work was supported by the High Technology Research and Development Pro-gram of China(Grant Nos.2002AA207020 and 2001AA241024)the National Natural Science Foundation of China(Grant No.30370906).
文摘Heterosis for yield and its component traits between chromosome segments from IR24, an indica variety, and the counterparts from 02428, a japonica rice, was inves- tigated by using a hybrid F1 population composed of 63 com- binations between 02428 and IR24 chromosome segment substitution lines (CSSLs) with the genetic background of Asominori, a japonica variety. Significant differences in het- erosis for yield and yield-component traits were observed among the crosses. Analysis of graphical genotyping showed that 14 substituted segments were responsible for yield het- erosis. All of them were from all the 12 chromosomes of IR24 except chromosomes 8 and 10. Six segments at the intervals of RFLP markers, such as X132—G1340—R459, X48— C393A, R288—R1854, R2918—X52, X257—C1350 and R367 —X189-2—X24-2 on chromosomes 2, 3, 4, 11 and 12 respec- tively, had very significant heterosis for yield at the level of P ≤0.005 based on t-test, individually increasing the hybrid yield by more than 35% compared with the control cross “Asominori×02428”. Most of IR24 chromosome segments were found to have no significant hybrid effect for yield and yield-component traits, and one segment located at R2171 on chromosome 6 possessed significant negative effect with 27% of yield decrease. Advantages of using CSSLs in the heterosis studies were discussed and approaches of the partial and genome-wide exploitation of rice heterosis between indica and japonica by molecular marker-assisted selection were then proposed.
文摘对陆海BC4F3和BC4F3:4代换系群体的纤维产量与品质的表型性状进行初步评价分析,结果表明,群体代换系各性状平均值与轮回亲本中棉所45相近,但群体内部个体间仍存在丰富的遗传变异,其中不乏超越中棉所45的材料。从中筛选20个纤维品质突出单株(株行),2年的上半部平均长度高于30.00 mm,断裂比强度高于31.0 c N/tex,表现较好的一致性和稳定性,为进一步棉花纤维品质育种提供了选择材料。
文摘以综合性状优良的烤烟种质Y3为轮回亲本,抗烟草黑胫病（0号和1号生理小种）和赤星病的雪茄烟种质Beinhart 1000-1及优质烤烟品种K326为供体亲本,连续回交并结合分子标记辅助选择,构建国内外第一套由256个具有烤烟Y3遗传背景并渐渗有Beinhart 1000-1和K326染色体片段株系代换系群体。该群体携带377个代换片段,分布于烟草24条连锁群上。每个株系携带1~5个代换片段,代换片段长度介于0.05~36.88 c M,平均长度7.75 c M。代换片段重叠累加总长度为2922.57 c M,是烟草基因组总长度的2.61倍。代换片段覆盖总长度为1114.32 c M,烟草基因组覆盖率为99.45%。本研究构建的片段代换系可用于烟草基因定位、复杂性状的QTL分析和标记辅助选择育种。
