目的:观察栀子-连翘药对配伍前后对发热型大鼠清热、利胆方面的影响,证明其配伍可以明显增强清热、利胆作用。方法:采用背部皮下注射干酵母混悬液法建立大鼠发热模型。制备栀子、连翘、栀子-连翘药对水提取物冻干粉,临用前水溶解灌胃给...目的:观察栀子-连翘药对配伍前后对发热型大鼠清热、利胆方面的影响,证明其配伍可以明显增强清热、利胆作用。方法:采用背部皮下注射干酵母混悬液法建立大鼠发热模型。制备栀子、连翘、栀子-连翘药对水提取物冻干粉,临用前水溶解灌胃给药。将SD大鼠随机分为正常组、模型组、阳性药对照组及中药给药组,中药给药组分为栀子、连翘单药给药组及栀子-连翘药对给药组。中药提取物溶液浓度为10 m L·kg-1(相当于单味药生药量3 g·kg-1),阳性药对照组给药浓度为10 m L·kg-1,空白组、模型组灌胃给予等量的生理盐水。除正常组大鼠外,各组大鼠背部皮下注射15%的干酵母悬液10 m L·kg-1,建立大鼠发热模型。电子体温计记录大鼠0、1、4、5、6、7、8 h体温,同时收集1-1、1-2、2-4、4-6、6-8、8-12、12-24 h胆汁,观察配伍前后发热大鼠的体温和胆汁量变化。结果:栀子、连翘及其药对配伍均具有一定的降低大鼠体温的作用,二药配伍后降温效果优于单用栀子或连翘;栀子-连翘药对能够降低发热大鼠的体温(P<0.05或P<0.01),对正常大鼠的体温有一定影响;栀子与连翘配伍具有一定的促进发热大鼠胆汁分泌的作用,二药配伍后胆汁分泌效果明显优于单用栀子(P<0.05)。结论:栀子与连翘配伍在大鼠发热模型上的降温能力优于单药应用,但无显著性差异;而在促进胆汁分泌方面具有显著性差异,说明该药对配伍后利胆作用增强。展开更多
Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chines...Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chinese Medicines, Zhizi could dispel dampness and heat via the urine to execute its choleretic effects. However, the potential molecular mechanism has been still poorly clarified. Here, we investigated the effect of different dose of Zhizi aqueous extract powder（0.3 g/kg/d and 0.9 g/kg/d） on urinary excretion of bile acids（BAs）, and defined the potential mechanism via renal BAs efflux transporters Mrp2 and Mrp4 in normal rats.Methods： Male Wistar rats were orally administrated with 0.3 or 0.9 g/kg/d dose of Zhizi aqueous extract powder for 2 weeks, then body weight, serum aminotransferase, total BAs concentrations in liver, bile,serum, kidney and urine, 1 h bile flow, 12-h urinary volume, biliary and urinary excretion amount of total BAs as well as protein expression of major renal BAs efflux transporter Mrp2 and Mrp4, were all evaluated.Results： Zhizi especially the high dose of Zhizi aqueous extract powder could reduce hepatic total BAs concentration. Additionally, bile flow and biliary excretion had no significant difference, but the remarkable increasing urinary excretion of BAs and 2 to 3 folds up-regulated renal Mrp2 expression were observed after administrated with Zhizi as compared with the control group.Conclusion： The findings indicate that Zhizi reduces hepatic total BAs level by increasing urinary excretion rather than the biliary excretion of BAs, which, in turn ascribed to elevated protein expression of Mrp2 at apical membrane of renal tubular epithelial cells.展开更多
文摘目的:观察栀子-连翘药对配伍前后对发热型大鼠清热、利胆方面的影响,证明其配伍可以明显增强清热、利胆作用。方法:采用背部皮下注射干酵母混悬液法建立大鼠发热模型。制备栀子、连翘、栀子-连翘药对水提取物冻干粉,临用前水溶解灌胃给药。将SD大鼠随机分为正常组、模型组、阳性药对照组及中药给药组,中药给药组分为栀子、连翘单药给药组及栀子-连翘药对给药组。中药提取物溶液浓度为10 m L·kg-1(相当于单味药生药量3 g·kg-1),阳性药对照组给药浓度为10 m L·kg-1,空白组、模型组灌胃给予等量的生理盐水。除正常组大鼠外,各组大鼠背部皮下注射15%的干酵母悬液10 m L·kg-1,建立大鼠发热模型。电子体温计记录大鼠0、1、4、5、6、7、8 h体温,同时收集1-1、1-2、2-4、4-6、6-8、8-12、12-24 h胆汁,观察配伍前后发热大鼠的体温和胆汁量变化。结果:栀子、连翘及其药对配伍均具有一定的降低大鼠体温的作用,二药配伍后降温效果优于单用栀子或连翘;栀子-连翘药对能够降低发热大鼠的体温(P<0.05或P<0.01),对正常大鼠的体温有一定影响;栀子与连翘配伍具有一定的促进发热大鼠胆汁分泌的作用,二药配伍后胆汁分泌效果明显优于单用栀子(P<0.05)。结论:栀子与连翘配伍在大鼠发热模型上的降温能力优于单药应用,但无显著性差异;而在促进胆汁分泌方面具有显著性差异,说明该药对配伍后利胆作用增强。
基金supported by the Prof. Xinan Wu’s,Dr. Yuhui Wei’s and Mr Guoqiang Zhang’s National Natural Science Foundation of China (Number:81373494,81373927,81641142 and 81702853)
文摘Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chinese Medicines, Zhizi could dispel dampness and heat via the urine to execute its choleretic effects. However, the potential molecular mechanism has been still poorly clarified. Here, we investigated the effect of different dose of Zhizi aqueous extract powder（0.3 g/kg/d and 0.9 g/kg/d） on urinary excretion of bile acids（BAs）, and defined the potential mechanism via renal BAs efflux transporters Mrp2 and Mrp4 in normal rats.Methods： Male Wistar rats were orally administrated with 0.3 or 0.9 g/kg/d dose of Zhizi aqueous extract powder for 2 weeks, then body weight, serum aminotransferase, total BAs concentrations in liver, bile,serum, kidney and urine, 1 h bile flow, 12-h urinary volume, biliary and urinary excretion amount of total BAs as well as protein expression of major renal BAs efflux transporter Mrp2 and Mrp4, were all evaluated.Results： Zhizi especially the high dose of Zhizi aqueous extract powder could reduce hepatic total BAs concentration. Additionally, bile flow and biliary excretion had no significant difference, but the remarkable increasing urinary excretion of BAs and 2 to 3 folds up-regulated renal Mrp2 expression were observed after administrated with Zhizi as compared with the control group.Conclusion： The findings indicate that Zhizi reduces hepatic total BAs level by increasing urinary excretion rather than the biliary excretion of BAs, which, in turn ascribed to elevated protein expression of Mrp2 at apical membrane of renal tubular epithelial cells.
