Influence of temperature on egg incubation of the wild Chinese alligator was studied during the summer seasons of 1999 and 2000(July to October and August to September,respectively)at three observation sites, Zhuangto...Influence of temperature on egg incubation of the wild Chinese alligator was studied during the summer seasons of 1999 and 2000(July to October and August to September,respectively)at three observation sites, Zhuangtou of Xuanzhou County,Shuangkeng and Zhongqiao of Jingxian county, Anhui Province. One nest at each of the above sites was monitored, bearing 19,17 and 19 eggs,respectively. According to the width of the band on the eggs, we estimated the dates that the eggs were laid. We recorded both the nest temperature and atmosphere temperature with an automated temperature data logger (HOBO Temp) during the whole observation period.We found that the average nest temperature maintained around 25 26℃ during incubation throughout day and night,within over two thirds of time in each day the nest temperature being higher than the atmosphere temperature. Analysis of our data revealed that alligator nests have buffering effect on the inside temperature. Higher nest temperature (27 30℃)increased the hatching rate. It was also noticed that the hatching rate varied considerably from site to site(between 0 100%). We conclude that climate is an important factor influencing egg hatching in the wild, and prolonged rainy season which caused large temperature fluctuation can decrease the hatching rate of the alligator eggs.展开更多
The completely identical HMG-box motif of CaSox4 gene from both male and female genomic DNA of the Chinese alligator (Alligator sinensis) was cloned and sequenced by degenerate primer PCR. Compared with the human and ...The completely identical HMG-box motif of CaSox4 gene from both male and female genomic DNA of the Chinese alligator (Alligator sinensis) was cloned and sequenced by degenerate primer PCR. Compared with the human and mouse SRY, CaSox4 revealed 51% and 57% nucleotide homology respectively and 49% and 55% amino acid identity respectively. CaSox4 belongs to subgroup C of the Sox gene family. The GC content is 86% in the HMG-box region of the CaSox4 gene. Blast analysis showed that the CaSox4 gene shares 100% amino acid identity with human Sox4, bird SoxLF4, turtle Sra4 and lizard CvSox4 genes. CaSox4 may be orthologous with the human Sox4 gene. This indicates that CaSox4 gene shows the remarkable evolutionary conservation during the evolution of Alligator sinensis. The extensive sequence conservation of the Sox4 gene between reptiles, mammals and birds suggests major functional constraints.展开更多
文摘Influence of temperature on egg incubation of the wild Chinese alligator was studied during the summer seasons of 1999 and 2000(July to October and August to September,respectively)at three observation sites, Zhuangtou of Xuanzhou County,Shuangkeng and Zhongqiao of Jingxian county, Anhui Province. One nest at each of the above sites was monitored, bearing 19,17 and 19 eggs,respectively. According to the width of the band on the eggs, we estimated the dates that the eggs were laid. We recorded both the nest temperature and atmosphere temperature with an automated temperature data logger (HOBO Temp) during the whole observation period.We found that the average nest temperature maintained around 25 26℃ during incubation throughout day and night,within over two thirds of time in each day the nest temperature being higher than the atmosphere temperature. Analysis of our data revealed that alligator nests have buffering effect on the inside temperature. Higher nest temperature (27 30℃)increased the hatching rate. It was also noticed that the hatching rate varied considerably from site to site(between 0 100%). We conclude that climate is an important factor influencing egg hatching in the wild, and prolonged rainy season which caused large temperature fluctuation can decrease the hatching rate of the alligator eggs.
文摘The completely identical HMG-box motif of CaSox4 gene from both male and female genomic DNA of the Chinese alligator (Alligator sinensis) was cloned and sequenced by degenerate primer PCR. Compared with the human and mouse SRY, CaSox4 revealed 51% and 57% nucleotide homology respectively and 49% and 55% amino acid identity respectively. CaSox4 belongs to subgroup C of the Sox gene family. The GC content is 86% in the HMG-box region of the CaSox4 gene. Blast analysis showed that the CaSox4 gene shares 100% amino acid identity with human Sox4, bird SoxLF4, turtle Sra4 and lizard CvSox4 genes. CaSox4 may be orthologous with the human Sox4 gene. This indicates that CaSox4 gene shows the remarkable evolutionary conservation during the evolution of Alligator sinensis. The extensive sequence conservation of the Sox4 gene between reptiles, mammals and birds suggests major functional constraints.
基金Sponsored by NSFC (30240213,30470244)Wildlife Conservation Society ( WCS)+1 种基金Special Scholar Foundation from Anhui(04043049)Key Laboratory of Biotic Environment and Ecological safetyin Anhui Province
