Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zh...Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.展开更多
以大白菜pol型细胞质雄性不育系16C14花蕾为试验材料,采用SMART(switching mechanism at 5′end of RNA transcript)技术合成cDNA,利用DSN(duplex-specific nuclease)对所得cDNA进行均一化处理,之后与pDONR^(TM)/Zeo载体重组并转化DH10...以大白菜pol型细胞质雄性不育系16C14花蕾为试验材料,采用SMART(switching mechanism at 5′end of RNA transcript)技术合成cDNA,利用DSN(duplex-specific nuclease)对所得cDNA进行均一化处理,之后与pDONR^(TM)/Zeo载体重组并转化DH10B大肠杆菌菌株,构建了大白菜pol型雄性不育花蕾均一化cDNA文库。经检测,文库滴度为3.2×10~6 cfu?mL^(-1),库容量约为1.28×10~7 cfu,重组率97%,插入片段平均大小1.2 kb左右。随机挑选100个阳性菌斑进行测序,冗余度仅为3.7%,文库质量较高。本研究成功构建了大白菜胞质雄性不育系花蕾均一化cDNA文库,为进一步研究大白菜pol雄性不育相关分子机制奠定基础。展开更多
基金the research results of Humanities and Social Science Planning Fund Project of the Ministry of Education of P.R.China,titled“Research on the Books Publishing of Modern Chinese Library from the Perspective of Generalized Technology”(Project number:19YJA870014).
文摘Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.
