Hepatitis C virus(HCV)infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20%of the total population are infected.Initial infection is usually asym...Hepatitis C virus(HCV)infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20%of the total population are infected.Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma.The management of HCV infection should not only be focus on therapy,but also to screen carrier individuals in order to prevent transmission.In the present,molecular detection and quantification of HCV genome by real time polymerase chain reaction(PCR)represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens.However,real time PCR is a complicated approach and of limited distribution.On the other hand,isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care.In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.展开更多
With complex topographic and hydrological characteristics,the landslide-induced surge disaster chain readily develops in mountainous and gorge areas,posing a huge challenge for infrastructure construction.This landsli...With complex topographic and hydrological characteristics,the landslide-induced surge disaster chain readily develops in mountainous and gorge areas,posing a huge challenge for infrastructure construction.This landslide-induced surge disaster chain involves a complex fluid-solid coupling between the landslide mass and a water body and exhibits complex energy conversion and dissipation characteristics,which is challenging to deal with using traditional finite element analysis.In this study,the energy evolution characteristics in the whole process of the disaster chain were first investigated,and the momentum-conservation equations for different stages were established.Then,the two-phase doublepoint material point method(TPDP-MPM)was used to model the landslide-induced surge disaster chain,and an experiment involving block-induced surge was modeled and simulated to validate this method.Finally,three generalized models were established for the landslide-induced surge process in a U-shaped valley,including subaerial,partly submerged,and submarine scenarios.The interaction mechanism between the landslide mass and the water body in the disaster chain was revealed by defining the system energy conversion ratio and the mechanism of evolution of the disaster chain from the perspective of energy.The results help further evaluate the secondary disasters,given the submerged position of the landslide mass.展开更多
文摘Hepatitis C virus(HCV)infection represents a significant health problem and represents a heavy load on some countries like Egypt in which about 20%of the total population are infected.Initial infection is usually asymptomatic and result in chronic hepatitis that give rise to complications including cirrhosis and hepatocellular carcinoma.The management of HCV infection should not only be focus on therapy,but also to screen carrier individuals in order to prevent transmission.In the present,molecular detection and quantification of HCV genome by real time polymerase chain reaction(PCR)represent the gold standard in HCV diagnosis and plays a crucial role in the management of therapeutic regimens.However,real time PCR is a complicated approach and of limited distribution.On the other hand,isothermal DNA amplification techniques have been developed and offer molecular diagnosis of infectious dieses at point-of-care.In this review we discuss recombinase polymerase amplification technique and illustrate its diagnostic value over both PCR and other isothermal amplification techniques.
基金supported by the National Natural Science Foundation of China(Grant Nos.52179117 and U21A20159)the Youth Innovation Promotion Association of Chinese Academy of Sciences(CAS)(Grant No.2021325).
文摘With complex topographic and hydrological characteristics,the landslide-induced surge disaster chain readily develops in mountainous and gorge areas,posing a huge challenge for infrastructure construction.This landslide-induced surge disaster chain involves a complex fluid-solid coupling between the landslide mass and a water body and exhibits complex energy conversion and dissipation characteristics,which is challenging to deal with using traditional finite element analysis.In this study,the energy evolution characteristics in the whole process of the disaster chain were first investigated,and the momentum-conservation equations for different stages were established.Then,the two-phase doublepoint material point method(TPDP-MPM)was used to model the landslide-induced surge disaster chain,and an experiment involving block-induced surge was modeled and simulated to validate this method.Finally,three generalized models were established for the landslide-induced surge process in a U-shaped valley,including subaerial,partly submerged,and submarine scenarios.The interaction mechanism between the landslide mass and the water body in the disaster chain was revealed by defining the system energy conversion ratio and the mechanism of evolution of the disaster chain from the perspective of energy.The results help further evaluate the secondary disasters,given the submerged position of the landslide mass.
