Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy. In this study, a novel spongy poly- urethane material was...Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy. In this study, a novel spongy poly- urethane material was implanted in the rat C^-s epidural space to establish a rat model of chronic cervical spondylotic myelopathy. Diffusion tensor data were used to predict pathological changes. Results revealed that the fractional anisotropy value gradually decreased at 4, 24, and 72 hours and 1 week after injury in rat spinal cord, showing a time-dependent manner. Average diffusion coeffi- cient increased at 72 hours and 1 week after implantation. Hematoxylin-eosin staining and Luxol-fast-blue staining exhibited that the number of neurons in the anterior horn of the spinal cord gray matter and the nerve fiber density of the white matter gradually reduced with prolonged com- pression time. Neuronal loss was most significant at 1 week after injury. Results verified that the fractional anisotropy value and average diffusion coefficient reflected the degree of pathological change in the site of compression in rat models at various time points after chronic spinal cord compression injury, which potentially has a reference value in the early diagnosis of chronic cervical spondylotic myelopathy.展开更多
【目的】观察慢性压迫性颈脊髓损伤神经元和轴突中神经丝高度磷酸化和异常积聚的特征,探讨其介导体感诱发电位(SEP)变化的神经病理机制。【方法】将20只SD大鼠随机分为实验组(n=15)和对照组(n=5),实验组采用聚氨酯胶片建立脊髓背侧慢性...【目的】观察慢性压迫性颈脊髓损伤神经元和轴突中神经丝高度磷酸化和异常积聚的特征,探讨其介导体感诱发电位(SEP)变化的神经病理机制。【方法】将20只SD大鼠随机分为实验组(n=15)和对照组(n=5),实验组采用聚氨酯胶片建立脊髓背侧慢性压迫模型;BBB评分法和SEP评估造模前、后6个月脊髓功能变化;Luxol Fast Blue(LFB)染色观察脱髓鞘变性、免疫组织化学法观察磷酸化(SMI-31)和非磷酸化(SMI-32)神经丝在脊髓灰质前角神经元、轴突中的表达,并探讨其与SEP变化的关系。【结果】造模6个月后大鼠BBB评分均下降(P<0.001)、SEP潜伏期延长(P<0.05)、波幅下降(P<0.001);LFB染色强度显著降低(P<0.001);脊髓灰质前角SMI-32阳性神经元计数明显增多、染色强度增强,两组差异具有统计学意义(P<0.05);脊髓白质SMI-31染色强度升高(P<0.05)。【结论】神经丝在脊髓前角神经元的过度表达与高度磷酸化以及在轴突中异常聚积是导致轴突变性、神经元损伤和体感诱发电位异常的神经病理机制之一。展开更多
[目的]探讨颈椎前路减压融合术（anterior cervical discectomy and fusion,ACDF）中采用新型零切迹桥形锁定融合器ROI-C治疗脊髓型颈椎病的早期临床疗效。[方法]回顾性分析2013年1月～2014年3月采用颈椎新型零切迹桥形锁定融合器ROI-C...[目的]探讨颈椎前路减压融合术（anterior cervical discectomy and fusion,ACDF）中采用新型零切迹桥形锁定融合器ROI-C治疗脊髓型颈椎病的早期临床疗效。[方法]回顾性分析2013年1月～2014年3月采用颈椎新型零切迹桥形锁定融合器ROI-C治疗脊髓型颈椎病41例患者共61个颈椎节段的相关资料,男22例,女19例;年龄37～77岁,平均57.6岁。采用日本骨科协会（Japanese Orthopaedic Association scores,JOA）评分评价患者术前术后神经功能,视觉模拟评分法（visual analogue scale,VAS）评估患者术前术后颈部疼痛,在X线片或CT片上测量术前术后颈椎生理曲度（Cobb角）、手术椎间隙高度、椎间融合情况并记录手术时间、术中出血量及术中透视次数。[结果]41例患者术后均获得随访,随访时间平均14.3个月。手术时间平均（65.4±7.3）min/节段。术中出血量平均（18.6±2.4）ml/节段、术中透视次数平均（4.84±1.27）次。末次随访,患者JOA评分由术前（9.26±0.94）分提高到（14.08±1.33）分（P〈0.01）,VAS评分由术前（4.97±1.25）分降低到（1.86±1.06）分（P〈0.01）,Cobb角由术前（13.15±4.87）°提高到（23.25±3.79）°（P〈0.05）,手术椎间隙高度由术前的（5.83±1.12）mm提高到（8.33±1.37）mm（P〈0.05）。末次随访时手术节段椎间隙全部融合。没有气管食管损伤及喉返神经损伤等并发症发生。未发现融合器移位、沉降、松动、断裂等情况。[结论]颈椎新型桥形锁定融合器ROI-C应用于脊髓型颈椎病前路减压融合术,操作简便,手术时间短,出血少,固定牢固,可有效恢复颈椎生理曲度及椎间隙高度,并发症少,融合成功率较高。展开更多
基金supported by the Natural Science Foundation of Guangdong Province of China,No.S2011010004843the National Natural Science Foundation of China,No.30770679,31070941,30570572the Major Basic Research of Ministry of Science and Technology of China(973 Project),No.2010CB530004
文摘Magnetic resonance diffusion tensor imaging has been shown to quantitatively measure the early pathological changes in chronic cervical spondylotic myelopathy. In this study, a novel spongy poly- urethane material was implanted in the rat C^-s epidural space to establish a rat model of chronic cervical spondylotic myelopathy. Diffusion tensor data were used to predict pathological changes. Results revealed that the fractional anisotropy value gradually decreased at 4, 24, and 72 hours and 1 week after injury in rat spinal cord, showing a time-dependent manner. Average diffusion coeffi- cient increased at 72 hours and 1 week after implantation. Hematoxylin-eosin staining and Luxol-fast-blue staining exhibited that the number of neurons in the anterior horn of the spinal cord gray matter and the nerve fiber density of the white matter gradually reduced with prolonged com- pression time. Neuronal loss was most significant at 1 week after injury. Results verified that the fractional anisotropy value and average diffusion coefficient reflected the degree of pathological change in the site of compression in rat models at various time points after chronic spinal cord compression injury, which potentially has a reference value in the early diagnosis of chronic cervical spondylotic myelopathy.
文摘【目的】观察慢性压迫性颈脊髓损伤神经元和轴突中神经丝高度磷酸化和异常积聚的特征,探讨其介导体感诱发电位(SEP)变化的神经病理机制。【方法】将20只SD大鼠随机分为实验组(n=15)和对照组(n=5),实验组采用聚氨酯胶片建立脊髓背侧慢性压迫模型;BBB评分法和SEP评估造模前、后6个月脊髓功能变化;Luxol Fast Blue(LFB)染色观察脱髓鞘变性、免疫组织化学法观察磷酸化(SMI-31)和非磷酸化(SMI-32)神经丝在脊髓灰质前角神经元、轴突中的表达,并探讨其与SEP变化的关系。【结果】造模6个月后大鼠BBB评分均下降(P<0.001)、SEP潜伏期延长(P<0.05)、波幅下降(P<0.001);LFB染色强度显著降低(P<0.001);脊髓灰质前角SMI-32阳性神经元计数明显增多、染色强度增强,两组差异具有统计学意义(P<0.05);脊髓白质SMI-31染色强度升高(P<0.05)。【结论】神经丝在脊髓前角神经元的过度表达与高度磷酸化以及在轴突中异常聚积是导致轴突变性、神经元损伤和体感诱发电位异常的神经病理机制之一。
文摘[目的]探讨颈椎前路减压融合术（anterior cervical discectomy and fusion,ACDF）中采用新型零切迹桥形锁定融合器ROI-C治疗脊髓型颈椎病的早期临床疗效。[方法]回顾性分析2013年1月～2014年3月采用颈椎新型零切迹桥形锁定融合器ROI-C治疗脊髓型颈椎病41例患者共61个颈椎节段的相关资料,男22例,女19例;年龄37～77岁,平均57.6岁。采用日本骨科协会（Japanese Orthopaedic Association scores,JOA）评分评价患者术前术后神经功能,视觉模拟评分法（visual analogue scale,VAS）评估患者术前术后颈部疼痛,在X线片或CT片上测量术前术后颈椎生理曲度（Cobb角）、手术椎间隙高度、椎间融合情况并记录手术时间、术中出血量及术中透视次数。[结果]41例患者术后均获得随访,随访时间平均14.3个月。手术时间平均（65.4±7.3）min/节段。术中出血量平均（18.6±2.4）ml/节段、术中透视次数平均（4.84±1.27）次。末次随访,患者JOA评分由术前（9.26±0.94）分提高到（14.08±1.33）分（P〈0.01）,VAS评分由术前（4.97±1.25）分降低到（1.86±1.06）分（P〈0.01）,Cobb角由术前（13.15±4.87）°提高到（23.25±3.79）°（P〈0.05）,手术椎间隙高度由术前的（5.83±1.12）mm提高到（8.33±1.37）mm（P〈0.05）。末次随访时手术节段椎间隙全部融合。没有气管食管损伤及喉返神经损伤等并发症发生。未发现融合器移位、沉降、松动、断裂等情况。[结论]颈椎新型桥形锁定融合器ROI-C应用于脊髓型颈椎病前路减压融合术,操作简便,手术时间短,出血少,固定牢固,可有效恢复颈椎生理曲度及椎间隙高度,并发症少,融合成功率较高。
