The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (...The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.展开更多
Based on the data of the Jiaozhou Bay Ecosystem Dynamic Research, cell volume and sur face area of 87 common phytoplankton species in China sea waters were calculated with assignment of the similar geometric form. The...Based on the data of the Jiaozhou Bay Ecosystem Dynamic Research, cell volume and sur face area of 87 common phytoplankton species in China sea waters were calculated with assignment of the similar geometric form. The cell plasma volume, live weight, Carbon content and nitrogen content were also calculated with the methods of Mullin et al. (1966), Strathmann (1967), Eppley et al. (1970), and Taguchi (1976). After comparing these methods, we chase the method of Eppley et al. (1970) as the hot method for calculating phytoplankton carbon content in China sea waters.展开更多
A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture o...A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture of material. This treatment method is used in the important computational region such as the material interface,large deformation region and fracture region where more particles are added for calculation,while the continuous transport method is used in the other regions. Through this method,a series of penetration problems such as blunt projectile penetration into the steel target and steel projectile penetration into concert target are numerically simulated. The comparison of the numerical result and experimental result shows that the hybrid algorithm can effectively track the deformation in the process of penetration and also insure precision and efficiency.展开更多
基金This work was supported by grants from the Wellcome Trust UK(056909/299/Z)Ministry of Education of China(GJ9901)+1 种基金the Health Department of Guangdong Province(A2001474)Guangdong Medical College Fund for Young Scholars(XQ0306).
文摘The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.
文摘Based on the data of the Jiaozhou Bay Ecosystem Dynamic Research, cell volume and sur face area of 87 common phytoplankton species in China sea waters were calculated with assignment of the similar geometric form. The cell plasma volume, live weight, Carbon content and nitrogen content were also calculated with the methods of Mullin et al. (1966), Strathmann (1967), Eppley et al. (1970), and Taguchi (1976). After comparing these methods, we chase the method of Eppley et al. (1970) as the hot method for calculating phytoplankton carbon content in China sea waters.
基金supported by the National Natural Science Foundation of China (Grant Nos. 10625208 and 10872034)the National Basic Research Program of China (Grant No. 2010CB8327006)the Foundation of State Key Laboratory of Explosion Science and Technology (Grant No. ZDKT08-02)
文摘A hybrid VOF and PIC multi-material interface treatment method for Eulerian method is presented in this study in order to solve the problem that the Eulerian method is not robust enough to treat the dynamic fracture of material. This treatment method is used in the important computational region such as the material interface,large deformation region and fracture region where more particles are added for calculation,while the continuous transport method is used in the other regions. Through this method,a series of penetration problems such as blunt projectile penetration into the steel target and steel projectile penetration into concert target are numerically simulated. The comparison of the numerical result and experimental result shows that the hybrid algorithm can effectively track the deformation in the process of penetration and also insure precision and efficiency.
