Background Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myoc...Background Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myocardial infarction. Methods The left anterior descending artery in adult female Sprague-Dawley （SD） rats was ligated to make a myocardial infarction model. Neonatal ventricular cardiomyocytes from one-day male SD rats were isolated, labeled and cultured. The cells were injected into the infarcted area three weeks later. The animals were randomized into four recipient groups： （1） cardiomyocytes plus fibrin sealant （group CF, n=10）; （2） cardiomyocytes alone （group C, n=10）; （3） fibrin sealant recipients alone （group F, n=10）; （4） control group （n=10）. Four weeks after transplantation, echocardiography and Langerdoff model were used to assess heart function. Immunohistochemical staining and polymerase chain reaction （PCR） were performed to track the implanted cardiomyocytes and detect the sex-determining region Y gene on Y chromosome. Results Echocardiography showed the fraction shortening （FS） in groups CF, C, F and control group was （27.80±6.32）%, （22.29±4.54）%, （19.24±6.29）% and （20.36±3.29）% respectively with statistically significant differences in group CF compared with the other groups （P〈0.05）. The Langendoff model revealed that the left ventricular development of peak pressure （LVDPmax, mmHg） in groups CF, C, F and control group was 104.81±17.05, 80.97±21.60, 72.07±26.17 and 71.42±17.55 respectively with statistically significant differences in group CF compared with the other groups （P〈0.05）. Pathological examination and PCR indicated that transplanted cardiomyocytes in group CF survived better than those in the other groups. Conclusion Transplanted neonatal cardiomyocytes plus fibrin sealant can survive in myocardial infarctioned area and improve heart function greatly in rat models.展开更多
Carbon deposition is a primary concern during the operation of solid oxide fuel cells（SOFCs） fueled with hydrocarbon fuels, leading to cell degradation and even cell damage. Carbon elimination is expected to be a pr...Carbon deposition is a primary concern during the operation of solid oxide fuel cells（SOFCs） fueled with hydrocarbon fuels, leading to cell degradation and even cell damage. Carbon elimination is expected to be a promising approach to prolong cell life. This work reports on a combined experimental and theoretical investigation of cell regeneration from anode carbon deposition of tubular SOFCs fabricated by phase-inversion and co-sintering techniques. The as-prepared cell exhibits a maximum power density of 0.20 W cm;at 800 ℃ fueling with wet CH;, but fails to stable operation due to severe carbon deposition.Based on thermodynamic predictions, a successive cell-protecting regeneration process is proposed to eliminate deposited carbon without oxidizing Ni catalysts, during which CH;and H;fuels are provided in circulation. Through a total of 35 cycling tests, cell performance can always successfully restore to the initial level.The possible carbon elimination mechanism is investigated in detail based on thermodynamic and first-principle calculations. The feasibility of carbon elimination using in situ produced oxygen or steam through electrochemical reaction has been revealed, providing a novel continuous operation mode for hydrocarbon-based SOFCs.展开更多
锌指蛋白32(zinc finger protein 32, ZNF32)是近几年发现的一种转录因子,与肿瘤的发生发展密切相关。本文综述了ZNF32在促进斑马鱼毛细胞再生、抗氧化应激以及在肿瘤细胞中调控肿瘤细胞自噬、诱导耐药性和促进复发和转移的功能,以期为...锌指蛋白32(zinc finger protein 32, ZNF32)是近几年发现的一种转录因子,与肿瘤的发生发展密切相关。本文综述了ZNF32在促进斑马鱼毛细胞再生、抗氧化应激以及在肿瘤细胞中调控肿瘤细胞自噬、诱导耐药性和促进复发和转移的功能,以期为后续的深入研究提供理论依据。展开更多
目的:研究复方葛根片对老龄大鼠肝细胞再生与凋亡的影响。方法:40只大鼠随机分为正常组、模型组(生理盐水,10ml/kg)和复方葛根片低、中、高剂量组[200、400、800 mg(生药)/kg],每组8只。除正常组外,其他各组大鼠每天上午9:00 ip D-半乳...目的:研究复方葛根片对老龄大鼠肝细胞再生与凋亡的影响。方法:40只大鼠随机分为正常组、模型组(生理盐水,10ml/kg)和复方葛根片低、中、高剂量组[200、400、800 mg(生药)/kg],每组8只。除正常组外,其他各组大鼠每天上午9:00 ip D-半乳糖溶液[250 mg/(kg·d)]复制衰老模型,每天下午15:00 ig相应药物,连续给药8周。按相关试剂盒操作分别测定血清、肝脏中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及肝脏中脂褐素(LPF)含量,并观察各组大鼠肝脏组织病理变化。结果:与正常组比较,模型组大鼠血清和肝组织中SOD活性显著降低,MDA、LPF含量显著升高(P<0.01);肝脏淋巴细胞显著增加,散在凋亡细胞增多,淋巴细胞在汇管区灶性浸润、肝血窦散在浸润。与模型组比较,复方葛根片低、高剂量组大鼠血清中SOD活性显著升高(P<0.05或P<0.01);中、高剂量组大鼠血清中MDA含量显著降低(P<0.01),肝组织中SOD活性显著升高(P<0.05或P<0.01);低、中、高剂量组大鼠肝组织中MDA、LPF含量显著降低(P<0.05或P<0.01);肝脏淋巴细胞减少,凋亡细胞少见,双核细胞显著增多。结论:复方葛根片能增加老龄大鼠肝细胞再生,减少其凋亡。展开更多
文摘Background Most cardiac regenerative approaches can restore injured heart muscles. In this study, we investigated if fibrin sealant could help neonatal cardiomyocytes restore myocardial function in a rat model of myocardial infarction. Methods The left anterior descending artery in adult female Sprague-Dawley （SD） rats was ligated to make a myocardial infarction model. Neonatal ventricular cardiomyocytes from one-day male SD rats were isolated, labeled and cultured. The cells were injected into the infarcted area three weeks later. The animals were randomized into four recipient groups： （1） cardiomyocytes plus fibrin sealant （group CF, n=10）; （2） cardiomyocytes alone （group C, n=10）; （3） fibrin sealant recipients alone （group F, n=10）; （4） control group （n=10）. Four weeks after transplantation, echocardiography and Langerdoff model were used to assess heart function. Immunohistochemical staining and polymerase chain reaction （PCR） were performed to track the implanted cardiomyocytes and detect the sex-determining region Y gene on Y chromosome. Results Echocardiography showed the fraction shortening （FS） in groups CF, C, F and control group was （27.80±6.32）%, （22.29±4.54）%, （19.24±6.29）% and （20.36±3.29）% respectively with statistically significant differences in group CF compared with the other groups （P〈0.05）. The Langendoff model revealed that the left ventricular development of peak pressure （LVDPmax, mmHg） in groups CF, C, F and control group was 104.81±17.05, 80.97±21.60, 72.07±26.17 and 71.42±17.55 respectively with statistically significant differences in group CF compared with the other groups （P〈0.05）. Pathological examination and PCR indicated that transplanted cardiomyocytes in group CF survived better than those in the other groups. Conclusion Transplanted neonatal cardiomyocytes plus fibrin sealant can survive in myocardial infarctioned area and improve heart function greatly in rat models.
基金supported financially by the Ministry of Science and Technology,China(No.MOST 2017YFB0601901 and 2017YFB0601903)Dongguan Science and Technology Bureau,Guangdong Project(No.201460720100025)Tsinghua University Initiative Scientific Research Program(No.2015THZ0)
文摘Carbon deposition is a primary concern during the operation of solid oxide fuel cells（SOFCs） fueled with hydrocarbon fuels, leading to cell degradation and even cell damage. Carbon elimination is expected to be a promising approach to prolong cell life. This work reports on a combined experimental and theoretical investigation of cell regeneration from anode carbon deposition of tubular SOFCs fabricated by phase-inversion and co-sintering techniques. The as-prepared cell exhibits a maximum power density of 0.20 W cm;at 800 ℃ fueling with wet CH;, but fails to stable operation due to severe carbon deposition.Based on thermodynamic predictions, a successive cell-protecting regeneration process is proposed to eliminate deposited carbon without oxidizing Ni catalysts, during which CH;and H;fuels are provided in circulation. Through a total of 35 cycling tests, cell performance can always successfully restore to the initial level.The possible carbon elimination mechanism is investigated in detail based on thermodynamic and first-principle calculations. The feasibility of carbon elimination using in situ produced oxygen or steam through electrochemical reaction has been revealed, providing a novel continuous operation mode for hydrocarbon-based SOFCs.
