AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected spec...AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected specimens of 361 gastric carcinomas(GC) were investigated with enzyme-, mucin-, and tumor-related marker immunohistochemistry. According to the direction of cell-function differentiation, stomach carcinomas were divided into five functionally differentiated types. RESULTS: (1) Absorptive function differentiation type (AFDT): there were 82 (22.7%) patients including 76 (92.7%) aged 45 years. Sixty-nine (84.1%) cases belonged to the intestinal type. Thirty-eight (46.3%) expressed CD44v6 and 9 (13.6%) of 66 male patients developed liver metastasis.The 5-year survival rate of patients in this group (58.5%) was higher than those with the other types (P【0.01). (2) Mucin secreting function differentiation type (MSFDT): 54 (15%) cases. Fifty-three (98.1%) tumors had penetrated the serosa, 12 (22.2%) expressed ER and 22 (40.7%) expressed CD44v6. The postoperative 5-year survival rate was 28.6%. (3) Absorptive and mucin-producing function differentiation type (AMPFDT): there were 180 (49.9%) cases, including 31 (17.2%) aged younger than 45 years. The tumor was more common in women (62, 34.4%,) and expressed more frequently estrogen receptors (ER) (129, 81.7%) than other types (P【0.01). Ovary metastasis was found in 12 (19.4%) out of 62 female subjects. The patients with this type GC had the lowest 5-year survival rate (24.7%) among all types. (4) Specific function differentiation type (SFDT): 13 (3.6%) cases. Nine (69.2%) tumors of this type derived from APUD system, the other 4 (30.7%) were of different histological differentiation. Sixty per cent of the patients survived at least five years. (5) Non-function differentiation type (NFDT): 32 (8.9%) cases. Nineteen (59.4%) cases had lymph node metastases but no one with liver or ovary metastasis. The 5-year survival rate was 28.1%. CONCLUSION: This new cell-f展开更多
Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) ...Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) culture supernatants dealt with by different concentrations of LianBiZhi (LBZ) injection, the effective component of which is andrographolide, were detected by biological activity test or ELISA in vitro. The effects of LBZ injection on macrophage phagocytotic function and natural killer cells cytotoxicity were examined by means of macrophage to phagocytize cock erythrocyte and measurement of lactate dehydrogenase (LDH) activity released from the damaged cells, respectively.Results: The LBZ injection could not only enhance the phagocytosis activity of peritoneal macrophage from guinea pig to phagocytosis cock erythrocytes, but also augment the cytotoxicity mediated by natural killer cells from PBMCs.Conclusion: Andrographolide is an immunostimulant agent which can modulate both antigen specific and nonspecific immune function by means of its natural killer cells, macrophage and cytokines.展开更多
Objective： To study the changes in cardiac function of rheumatoid arthritis （RA） patients and to observe the effect of Xinfeng Capsule （新风胶囊 XFC） on them. Methods： Sixty-eight RA patients were randomly assig...Objective： To study the changes in cardiac function of rheumatoid arthritis （RA） patients and to observe the effect of Xinfeng Capsule （新风胶囊 XFC） on them. Methods： Sixty-eight RA patients were randomly assigned to two groups by a lottery： 38 patients in the treatment group treated orally with XFC, 3 capsules, thrice a day, and 30 in the control group treated with Fengshi Gutong Capsule （风湿骨痛胶囊, FSGTC）, 4 capsules, twice a day, 30 days as one course of treatment, and two courses were given for both groups. A normal control （NC） group including 20 healthy subjects was set up. The clinical efficacy was compared between the two treated groups. The changes in cardiac function, including early diastolic peak flow velocity （E）, late diastolic peak flow velocity （A）, left ventricular fraction shortening （FS）, and E/ A, as well as uric acid （UA）, erythrocyte sedimentation rate （ESR）, α-acid glycoprotein （α-AGP）, and hypersensitive C-reaction protein （hs-CRP）, were observed. The regulation T cell was determined with flow cytometry. Results： （1） The total effective rate in the treatment group and the control group was 92.1% （35/38） and 70.0% （21/30）, respectively. Significant difference was shown between them （P〈0.05）. （2） Compared with those of the NC group, E peak, E/A ratio, and FS of RA patients were lower （P〈0.01）, while A peak was higher （P〈0.01）. Moreover, A peak of the treatment group after treatment was significantly lower （P〈0.05） and E/A ratio was significantly higher （P〈0.05） as compared with those of the control group. （3） The improvement in the treatment group in reducing UA and hs-CRP was superior to those of the control group （P〈0.05）. In addition, the improvement in α-AGP, CD4＋CD25＋ Treg, and CD4＋CD25＋CD12T Treg of the treatment group was obvious as compared with the control group, although the difference was not statistically significant. （Conclusions： The descenden展开更多
Background Human umbilical cord blood contains an abundance of immature stem/progenitor cells, which may participate in the repair of hearts that have been damaged by myocardial infarction （MI）. This study aimed to ...Background Human umbilical cord blood contains an abundance of immature stem/progenitor cells, which may participate in the repair of hearts that have been damaged by myocardial infarction （MI）. This study aimed to evaluate the effects of human umbilical cord blood mononuclear cells （hUCBC） transplantation on cardiac function and left ventricular remodeling in rat model of MI. Methods Forty-five male Wistar rats were randomized into three groups： MI or control group （n=15）, MI plus cell transplantation （n=15）, and sham group （n=15）. Acute myocardial infarction （AMI） was established by ligating the left anterior descending artery, thereafter, hUCBC were implanted into the marginal area of infarcted myocardium. In MI/control group, DMEM was injected instead of hUCBC following the same protocol. Left ventricular function assessment was carded out by echocardiography and invasive hemodynamic measurements one month post MI. All rats were sacrificed for histological and immunochemical examinations.Results The transplanted hUCBC survived and engaged in the process of myocardial repair in the host heart. Echocardiography demonstrated that left ventricular function improved significantly in the rats that underwent cell transplantation. Hemodynamic studies found a significantly decreased left ventricular end-diastolic pressure （LVEDP） [（21.08±8.10） mmHg vs （30.82±9.59） mmHg, P〈0.05], increase in ＋dp/dtmax [（4.29± 1.27） mmHg/ms vs （3.24±0.75） mmHg/ms, P〈0.05）, and increase in -dp/dtmax [（3.71 ±0.79） mmHg/ms vs （3.00± 0.49） mmHg/ms, P〈0.05] among MI group with hUCBC transplantation when compared with MI/control group. Masson＇s trichrome staining revealed that the collagen density in the left ventricle was significantly lower in rats of transplantation group than that in the MI control groups [（6.33±2.69）% vs （11.10±3.75）%, P〈 0.01]. Based on immunostaining of α-actin, the numbers of microvessels were significantly （P〈0.01） increased at展开更多
Objective： To evaluate the effects of the Chinese herbal formula Wuzi Yanzong Pill （五子衍宗丸, WYP） on the spermatogenesis and specific secretory functions of Sertoli cells in rat model and to investigate the unde...Objective： To evaluate the effects of the Chinese herbal formula Wuzi Yanzong Pill （五子衍宗丸, WYP） on the spermatogenesis and specific secretory functions of Sertoli cells in rat model and to investigate the underlying mechanism. Me^ods： Five groups of male Sprague-Dawley rats including the control group, the model group, the low-dose WYP group, the medium-dose WYP group and the high-dose WYP group （5 in each group） were treated daily with vehicle, multiglycosides of Tripterygium wilfordii Hook f （GTW） either alone （20 mg/kg） or followed by WYP （0.5, 1.0, or 2.0 g/kg daily）, respectively for 30 days. Serum levels of follicle-stimulating hormone （FSH）, inhibin B （INHB） and testosterone （r） were evaluated using enzyme-linked immunosorbent assay. Androgen- binding protein （ABP） gene expression and transferrin （TF） protein expression in testis tissue specimens of all rats were determined using real-time reverse transcriptase polymerase chain reaction and Western blotting analysis, respectively. Histopathological alterations in the testis were determined using Johnsen＇s score. Results： The toxicity of GTW towards Sertoli cell secretory functions and spermatogenesis was accompanied by increased serum FSH concentrations and decreased INHB and T concentrations. Upregulated ABP mRNA levels, and decreased TF protein expression and Johnsen＇s scores were detected in the model group compared with the control group （P〈0.05 or P〈0.01）. Oral high-dose WYP administrations to GTW-treated rats effectively alleviated all of the GTW- induced changes in specific secretory functions of Sertoli cells （ABP, INHB and TF）. Furthermore, serum T level and Johnsen＇s score of the testis increased greatly compared with the model group （P〈0.01）. Conclusion： WYP has the ability to improve the spermatogenesis, possibly through modulating the secretory proteins expression of Sertoli cells.展开更多
基金Project supported by the National Natural Science Foundation of China, No. 39270300. No. 39370772Training Program for Trans-Century Talents by the State Education Commission of China
文摘AIM: To reveal the correlation between the functional differentiation phenotypes of gastric carcinoma cells and the invasion and metastasis by a new way of cell-function classification.METHODS:Surgically resected specimens of 361 gastric carcinomas(GC) were investigated with enzyme-, mucin-, and tumor-related marker immunohistochemistry. According to the direction of cell-function differentiation, stomach carcinomas were divided into five functionally differentiated types. RESULTS: (1) Absorptive function differentiation type (AFDT): there were 82 (22.7%) patients including 76 (92.7%) aged 45 years. Sixty-nine (84.1%) cases belonged to the intestinal type. Thirty-eight (46.3%) expressed CD44v6 and 9 (13.6%) of 66 male patients developed liver metastasis.The 5-year survival rate of patients in this group (58.5%) was higher than those with the other types (P【0.01). (2) Mucin secreting function differentiation type (MSFDT): 54 (15%) cases. Fifty-three (98.1%) tumors had penetrated the serosa, 12 (22.2%) expressed ER and 22 (40.7%) expressed CD44v6. The postoperative 5-year survival rate was 28.6%. (3) Absorptive and mucin-producing function differentiation type (AMPFDT): there were 180 (49.9%) cases, including 31 (17.2%) aged younger than 45 years. The tumor was more common in women (62, 34.4%,) and expressed more frequently estrogen receptors (ER) (129, 81.7%) than other types (P【0.01). Ovary metastasis was found in 12 (19.4%) out of 62 female subjects. The patients with this type GC had the lowest 5-year survival rate (24.7%) among all types. (4) Specific function differentiation type (SFDT): 13 (3.6%) cases. Nine (69.2%) tumors of this type derived from APUD system, the other 4 (30.7%) were of different histological differentiation. Sixty per cent of the patients survived at least five years. (5) Non-function differentiation type (NFDT): 32 (8.9%) cases. Nineteen (59.4%) cases had lymph node metastases but no one with liver or ovary metastasis. The 5-year survival rate was 28.1%. CONCLUSION: This new cell-f
文摘Objective: To study the effects of andrographolide on immune functions and the immune mechanism in clinical therapy.Methods: The amounts of IFN-α,IFN-γ, TNF-α, IL-8 in the peripheral blood mononuclear cells (PBMC) culture supernatants dealt with by different concentrations of LianBiZhi (LBZ) injection, the effective component of which is andrographolide, were detected by biological activity test or ELISA in vitro. The effects of LBZ injection on macrophage phagocytotic function and natural killer cells cytotoxicity were examined by means of macrophage to phagocytize cock erythrocyte and measurement of lactate dehydrogenase (LDH) activity released from the damaged cells, respectively.Results: The LBZ injection could not only enhance the phagocytosis activity of peritoneal macrophage from guinea pig to phagocytosis cock erythrocytes, but also augment the cytotoxicity mediated by natural killer cells from PBMCs.Conclusion: Andrographolide is an immunostimulant agent which can modulate both antigen specific and nonspecific immune function by means of its natural killer cells, macrophage and cytokines.
基金Supported by the National Key Discipline of Traditional Chinese Medicine Project for Bi Diseases[Administration of Traditional Chinese Medicine,China(2009) No.30]Program of Science and Technology Department of Anhui Province,China(No. 07010300204)Traditional Chinese Medicine Research Project of Health Department of Anhui Province,China(No. 2009ZY05)
文摘Objective： To study the changes in cardiac function of rheumatoid arthritis （RA） patients and to observe the effect of Xinfeng Capsule （新风胶囊 XFC） on them. Methods： Sixty-eight RA patients were randomly assigned to two groups by a lottery： 38 patients in the treatment group treated orally with XFC, 3 capsules, thrice a day, and 30 in the control group treated with Fengshi Gutong Capsule （风湿骨痛胶囊, FSGTC）, 4 capsules, twice a day, 30 days as one course of treatment, and two courses were given for both groups. A normal control （NC） group including 20 healthy subjects was set up. The clinical efficacy was compared between the two treated groups. The changes in cardiac function, including early diastolic peak flow velocity （E）, late diastolic peak flow velocity （A）, left ventricular fraction shortening （FS）, and E/ A, as well as uric acid （UA）, erythrocyte sedimentation rate （ESR）, α-acid glycoprotein （α-AGP）, and hypersensitive C-reaction protein （hs-CRP）, were observed. The regulation T cell was determined with flow cytometry. Results： （1） The total effective rate in the treatment group and the control group was 92.1% （35/38） and 70.0% （21/30）, respectively. Significant difference was shown between them （P〈0.05）. （2） Compared with those of the NC group, E peak, E/A ratio, and FS of RA patients were lower （P〈0.01）, while A peak was higher （P〈0.01）. Moreover, A peak of the treatment group after treatment was significantly lower （P〈0.05） and E/A ratio was significantly higher （P〈0.05） as compared with those of the control group. （3） The improvement in the treatment group in reducing UA and hs-CRP was superior to those of the control group （P〈0.05）. In addition, the improvement in α-AGP, CD4＋CD25＋ Treg, and CD4＋CD25＋CD12T Treg of the treatment group was obvious as compared with the control group, although the difference was not statistically significant. （Conclusions： The descenden
基金This study was supported by the Research Fund of the Department of Guangdong Science and Technology （No. 2003C30603） and Natural Science Foundation of Guangdong （No. 5001680）.
文摘Background Human umbilical cord blood contains an abundance of immature stem/progenitor cells, which may participate in the repair of hearts that have been damaged by myocardial infarction （MI）. This study aimed to evaluate the effects of human umbilical cord blood mononuclear cells （hUCBC） transplantation on cardiac function and left ventricular remodeling in rat model of MI. Methods Forty-five male Wistar rats were randomized into three groups： MI or control group （n=15）, MI plus cell transplantation （n=15）, and sham group （n=15）. Acute myocardial infarction （AMI） was established by ligating the left anterior descending artery, thereafter, hUCBC were implanted into the marginal area of infarcted myocardium. In MI/control group, DMEM was injected instead of hUCBC following the same protocol. Left ventricular function assessment was carded out by echocardiography and invasive hemodynamic measurements one month post MI. All rats were sacrificed for histological and immunochemical examinations.Results The transplanted hUCBC survived and engaged in the process of myocardial repair in the host heart. Echocardiography demonstrated that left ventricular function improved significantly in the rats that underwent cell transplantation. Hemodynamic studies found a significantly decreased left ventricular end-diastolic pressure （LVEDP） [（21.08±8.10） mmHg vs （30.82±9.59） mmHg, P〈0.05], increase in ＋dp/dtmax [（4.29± 1.27） mmHg/ms vs （3.24±0.75） mmHg/ms, P〈0.05）, and increase in -dp/dtmax [（3.71 ±0.79） mmHg/ms vs （3.00± 0.49） mmHg/ms, P〈0.05] among MI group with hUCBC transplantation when compared with MI/control group. Masson＇s trichrome staining revealed that the collagen density in the left ventricle was significantly lower in rats of transplantation group than that in the MI control groups [（6.33±2.69）% vs （11.10±3.75）%, P〈 0.01]. Based on immunostaining of α-actin, the numbers of microvessels were significantly （P〈0.01） increased at
基金Supported by National Natural Science Foundation of China(No.81072809 and 81273767)Beijing Natural Science Foundation(No.7102140)
文摘Objective： To evaluate the effects of the Chinese herbal formula Wuzi Yanzong Pill （五子衍宗丸, WYP） on the spermatogenesis and specific secretory functions of Sertoli cells in rat model and to investigate the underlying mechanism. Me^ods： Five groups of male Sprague-Dawley rats including the control group, the model group, the low-dose WYP group, the medium-dose WYP group and the high-dose WYP group （5 in each group） were treated daily with vehicle, multiglycosides of Tripterygium wilfordii Hook f （GTW） either alone （20 mg/kg） or followed by WYP （0.5, 1.0, or 2.0 g/kg daily）, respectively for 30 days. Serum levels of follicle-stimulating hormone （FSH）, inhibin B （INHB） and testosterone （r） were evaluated using enzyme-linked immunosorbent assay. Androgen- binding protein （ABP） gene expression and transferrin （TF） protein expression in testis tissue specimens of all rats were determined using real-time reverse transcriptase polymerase chain reaction and Western blotting analysis, respectively. Histopathological alterations in the testis were determined using Johnsen＇s score. Results： The toxicity of GTW towards Sertoli cell secretory functions and spermatogenesis was accompanied by increased serum FSH concentrations and decreased INHB and T concentrations. Upregulated ABP mRNA levels, and decreased TF protein expression and Johnsen＇s scores were detected in the model group compared with the control group （P〈0.05 or P〈0.01）. Oral high-dose WYP administrations to GTW-treated rats effectively alleviated all of the GTW- induced changes in specific secretory functions of Sertoli cells （ABP, INHB and TF）. Furthermore, serum T level and Johnsen＇s score of the testis increased greatly compared with the model group （P〈0.01）. Conclusion： WYP has the ability to improve the spermatogenesis, possibly through modulating the secretory proteins expression of Sertoli cells.
