Transmission Electron Microscope (TEM) Technology was used to investigate the effect of 25,100 and 200 mg/kg copper on ultra-structure of root tip and leaf blade of wheat. Result showed that serious damage was found w...Transmission Electron Microscope (TEM) Technology was used to investigate the effect of 25,100 and 200 mg/kg copper on ultra-structure of root tip and leaf blade of wheat. Result showed that serious damage was found with Copper of 25,100 and 200 mg/kg. Plasmolysis,concentrated cytoplasm,chloroplast inflation,lamellar structure disturbance,capsule disappearance and disintegration,mitochondria structures ambiguity and vacuolization were all symptoms under Cu stress. There were positive correlation between concentration of coper stress and the degree of injury,and the degree of injury of copper were different in different organelles. Mitochondria were the most sensitive organelles,and there was patient difference in the same organelles of different parts.展开更多
Surgical treatment of peripheral nerve injuries is still a major challenge in human clinic.Up to now,none of the well-developed microsurgical treatment options is able to guarantee a complete restoration of nerve func...Surgical treatment of peripheral nerve injuries is still a major challenge in human clinic.Up to now,none of the well-developed microsurgical treatment options is able to guarantee a complete restoration of nerve function.This restriction is also effective for novel clinically approved artificial nerve guides.In this review,we compare surgical repair techniques primarily for digital nerve injuries reported with relatively high prevalence to be valuable attempts in clinical digital nerve repair and point out their advantages and shortcomings.We furthermore discuss the use of artificial nerve grafts with a focus on chitosan-based nerve guides,for which our own studies contributed to their approval for clinical use.In the second part of this review,very recent future perspectives for the enhancement of tubular(commonly hollow)nerve guides are discussed in terms of their clinical translatability and ability to form three-dimensional constructs that biomimick the natural nerve structure.This includes materials that have already shown their beneficial potential in in vivo studies like fibrous intraluminal guidance structures,hydrogels,growth factors,and approaches of cell transplantation.Additionally,we highlight upcoming future perspectives comprising co-application of stem cell secretome.From our overview,we conclude that already simple attempts are highly effective to increase the regeneration supporting properties of nerve guides in experimental studies.But for bringing nerve repair with bioartificial nerve grafts to the next level,e.g.repair of defects>3 cm in human patients,more complex intraluminal guidance structures such as innovatively manufactured hydrogels and likely supplementation of stem cells or their secretome for therapeutic purposes may represent promising future perspectives.展开更多
Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have ...Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have developed a cell-imprinted polydimethylsiloxane(PDMS) with aptamer functionalization(APTCIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9% ± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields.展开更多
Abstract： In the present study was investigated Arg-X protease-sensitive in supramolecular-genome compartments （nucleoplasm, chromatin, nuclear matrix）, during the period of the transcriptional activation of chroma...Abstract： In the present study was investigated Arg-X protease-sensitive in supramolecular-genome compartments （nucleoplasm, chromatin, nuclear matrix）, during the period of the transcriptional activation of chromatin when the growth processes was initiated in the mature germs of winter and transformed from it spring wheat. The germs have been separated from endosperm from 0 h （air-dry seed） up to 21 h in each 3 h after the start of seeds soaking. Cell nucleus have been allocated from germs and cleared, and then from them supramolecular-genome compartments were extracted by increasing ionic strength of solution. The Arg-X （tryptase） activity was assessed by cleavage of Arg-X bonds in the arginine-enriched protein protamine in all nuclear fractions. In the present study have shown what Arg-X protease-sensitives zones can be located on the supramolecular structures of chromatin matrix in processes of realization of ontogenetic programs of development in mature germs of the winter and transformed from it spring wheat. Arg-X protease-sensitive can translocate and coordinated in heteropolymer structures on the same genetic matrix. Questions of epigenetic mechanisms are discussed.展开更多
Sandwich construction incorporating a honeycomb cellular core offers the attainment of structures that are very stiff and strong in bending while the weight is kept at a minimum. Generally, an aluminum or Nomex honeyc...Sandwich construction incorporating a honeycomb cellular core offers the attainment of structures that are very stiff and strong in bending while the weight is kept at a minimum. Generally, an aluminum or Nomex honeycomb core is used in applications requiring sandwich construction with fiber-reinforced composite facesheets. However, the use of a fiber-reinforced composite core offers the potential for even lower weight, increased stiffness and strength, low thermal distortion compatible with that of the facesheets, the absence of galvanic corrosion and the ability to readily modify the core properties to suit specialized needs. Furthermore, the material of the core itself will exhibit anisotropic material properties in this case. In order to design, analyze and optimize these structures, knowledge of the effective mechanical properties of the core is essential. In this paper, the effective three-dimensional mechanical properties of a composite hexagonal cell core are determined using a numerical method based on a finite element analysis of a representative unit cell. In particular, the geometry of the simplest repeating unit of the core as well as the appropriate loading and boundary conditions that must be applied is presented.展开更多
Passive RFID tags based on narrow band absorbers are proposed for railway applications. The tags consist of absorbing tiles of size 30 × 30 cm2. The de-signs of these narrow band absorbers are presented based on ...Passive RFID tags based on narrow band absorbers are proposed for railway applications. The tags consist of absorbing tiles of size 30 × 30 cm2. The de-signs of these narrow band absorbers are presented based on infinite array approximation by using periodic boundary conditions. Then, the radar cross sections (RCS) of finite tiles of these designed absorbers are compared with the corresponding RCS of a conduction plate of the same size of the tiles. The difference between the RCS of the absorbing tiles and the conducting tiles corresponds to the RFID digit “1” or “0”. An experiential verification for a sample of these narrow band absorbers is presented based on measuring a fi-nite tile inside a transverse electromagnetic (TEM) cell. The design of this TEM cell is also discussed in detail.展开更多
Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for ...Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for fundamental cell biology research, but also for tissue engineering (TE). Currently, TE research adopts either the top-down or bottom-up approach. The top-down approach involves defining the macroscopic tissue features using biomaterial scaffolds and seeding cells into these scaffolds. Conversely, the bottom-up approach aims at crafting small tissue building blocks with precision-engineered structural and functional microscale features, using physical and/or chemical approaches. The bottom-up strategy takes advantage of the repeating structural and functional units that facilitate cell-cell interactions and cultures multiple cells together as a functional unit of tissue. In this review, we focus on currently available microscale methods that can control mammalian cells to assemble into 3D tissue-like structures.展开更多
It is of great significance to present a photonic crystal lattice structure with a wide photonic bandgap. A two-dimension complex lattice photonic crystal is proposed. The photonic crystal is composed of complex latti...It is of great significance to present a photonic crystal lattice structure with a wide photonic bandgap. A two-dimension complex lattice photonic crystal is proposed. The photonic crystal is composed of complex lattices with triangular structure, and each single cell is surrounded by six scatterers in an hexagon. The photonic band gaps are calculated based on the plane wave expansion (PWE) method. The results indicate that the photonic crystal has tunable large TM polarization band gap, and a gap-midgap ra...展开更多
The organized alignment of cells in various tissues plays a significant role in the maintenance of specific functions.To induce such an alignment,ideal scaffolds should simulate the characteristics and morphologies of...The organized alignment of cells in various tissues plays a significant role in the maintenance of specific functions.To induce such an alignment,ideal scaffolds should simulate the characteristics and morphologies of natural tissues.Aligned structures that guide cell orientation are used to facilitate tissue regeneration and repair.We here review how various aligned structures are fabricated,including aligned electrospun nanofibers,aligned porous or channeled structures,micropatterns and combinations thereof,and their application in nerve,skeletal muscle,tendon,and tubular dentin regeneration.The future use of aligned structures in tissue engineering is also discussed.展开更多
基金Supported by Scientific and Technological Fund from China University of Mining and Technology (D200402)~~
文摘Transmission Electron Microscope (TEM) Technology was used to investigate the effect of 25,100 and 200 mg/kg copper on ultra-structure of root tip and leaf blade of wheat. Result showed that serious damage was found with Copper of 25,100 and 200 mg/kg. Plasmolysis,concentrated cytoplasm,chloroplast inflation,lamellar structure disturbance,capsule disappearance and disintegration,mitochondria structures ambiguity and vacuolization were all symptoms under Cu stress. There were positive correlation between concentration of coper stress and the degree of injury,and the degree of injury of copper were different in different organelles. Mitochondria were the most sensitive organelles,and there was patient difference in the same organelles of different parts.
文摘Surgical treatment of peripheral nerve injuries is still a major challenge in human clinic.Up to now,none of the well-developed microsurgical treatment options is able to guarantee a complete restoration of nerve function.This restriction is also effective for novel clinically approved artificial nerve guides.In this review,we compare surgical repair techniques primarily for digital nerve injuries reported with relatively high prevalence to be valuable attempts in clinical digital nerve repair and point out their advantages and shortcomings.We furthermore discuss the use of artificial nerve grafts with a focus on chitosan-based nerve guides,for which our own studies contributed to their approval for clinical use.In the second part of this review,very recent future perspectives for the enhancement of tubular(commonly hollow)nerve guides are discussed in terms of their clinical translatability and ability to form three-dimensional constructs that biomimick the natural nerve structure.This includes materials that have already shown their beneficial potential in in vivo studies like fibrous intraluminal guidance structures,hydrogels,growth factors,and approaches of cell transplantation.Additionally,we highlight upcoming future perspectives comprising co-application of stem cell secretome.From our overview,we conclude that already simple attempts are highly effective to increase the regeneration supporting properties of nerve guides in experimental studies.But for bringing nerve repair with bioartificial nerve grafts to the next level,e.g.repair of defects>3 cm in human patients,more complex intraluminal guidance structures such as innovatively manufactured hydrogels and likely supplementation of stem cells or their secretome for therapeutic purposes may represent promising future perspectives.
基金financial support from the National Key Research and Development Program (No. 2016YFA0501401)National Natural Science Foundation of China (Nos. 21575143 and 91543201)+1 种基金CAS Key Project in Frontier Science(No. QYZDY-SSW-SLH017)Kaiguang Yang is the member of Youth Innovation Promotion Association, CAS(No. 2017222)
文摘Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have developed a cell-imprinted polydimethylsiloxane(PDMS) with aptamer functionalization(APTCIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9% ± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields.
文摘Abstract： In the present study was investigated Arg-X protease-sensitive in supramolecular-genome compartments （nucleoplasm, chromatin, nuclear matrix）, during the period of the transcriptional activation of chromatin when the growth processes was initiated in the mature germs of winter and transformed from it spring wheat. The germs have been separated from endosperm from 0 h （air-dry seed） up to 21 h in each 3 h after the start of seeds soaking. Cell nucleus have been allocated from germs and cleared, and then from them supramolecular-genome compartments were extracted by increasing ionic strength of solution. The Arg-X （tryptase） activity was assessed by cleavage of Arg-X bonds in the arginine-enriched protein protamine in all nuclear fractions. In the present study have shown what Arg-X protease-sensitives zones can be located on the supramolecular structures of chromatin matrix in processes of realization of ontogenetic programs of development in mature germs of the winter and transformed from it spring wheat. Arg-X protease-sensitive can translocate and coordinated in heteropolymer structures on the same genetic matrix. Questions of epigenetic mechanisms are discussed.
文摘Sandwich construction incorporating a honeycomb cellular core offers the attainment of structures that are very stiff and strong in bending while the weight is kept at a minimum. Generally, an aluminum or Nomex honeycomb core is used in applications requiring sandwich construction with fiber-reinforced composite facesheets. However, the use of a fiber-reinforced composite core offers the potential for even lower weight, increased stiffness and strength, low thermal distortion compatible with that of the facesheets, the absence of galvanic corrosion and the ability to readily modify the core properties to suit specialized needs. Furthermore, the material of the core itself will exhibit anisotropic material properties in this case. In order to design, analyze and optimize these structures, knowledge of the effective mechanical properties of the core is essential. In this paper, the effective three-dimensional mechanical properties of a composite hexagonal cell core are determined using a numerical method based on a finite element analysis of a representative unit cell. In particular, the geometry of the simplest repeating unit of the core as well as the appropriate loading and boundary conditions that must be applied is presented.
文摘Passive RFID tags based on narrow band absorbers are proposed for railway applications. The tags consist of absorbing tiles of size 30 × 30 cm2. The de-signs of these narrow band absorbers are presented based on infinite array approximation by using periodic boundary conditions. Then, the radar cross sections (RCS) of finite tiles of these designed absorbers are compared with the corresponding RCS of a conduction plate of the same size of the tiles. The difference between the RCS of the absorbing tiles and the conducting tiles corresponds to the RFID digit “1” or “0”. An experiential verification for a sample of these narrow band absorbers is presented based on measuring a fi-nite tile inside a transverse electromagnetic (TEM) cell. The design of this TEM cell is also discussed in detail.
基金supported by Ministry of Science and Technology of China(Grant Nos.2009CB930001 and 2011CB933201)Chinese Academy ofSciences(Grant No.KJCX2-YW-M15)the National Natural ScienceFoundation of China(Grant Nos.20890020,90813032,21025520 and 51073045)
文摘Different cell types make up tissues and organs hierarchically and communicate within a complex, three-dimensional (3D) en- vironment. The in vitro recapitulation of tissue-like structures is meaningful, not only for fundamental cell biology research, but also for tissue engineering (TE). Currently, TE research adopts either the top-down or bottom-up approach. The top-down approach involves defining the macroscopic tissue features using biomaterial scaffolds and seeding cells into these scaffolds. Conversely, the bottom-up approach aims at crafting small tissue building blocks with precision-engineered structural and functional microscale features, using physical and/or chemical approaches. The bottom-up strategy takes advantage of the repeating structural and functional units that facilitate cell-cell interactions and cultures multiple cells together as a functional unit of tissue. In this review, we focus on currently available microscale methods that can control mammalian cells to assemble into 3D tissue-like structures.
基金supported by the Special Foundation for Harb-in Young Scientists (Grant Number 2008RFQXG031)the Ba-sic Research Foundation of Harbin Engineering University.
文摘It is of great significance to present a photonic crystal lattice structure with a wide photonic bandgap. A two-dimension complex lattice photonic crystal is proposed. The photonic crystal is composed of complex lattices with triangular structure, and each single cell is surrounded by six scatterers in an hexagon. The photonic band gaps are calculated based on the plane wave expansion (PWE) method. The results indicate that the photonic crystal has tunable large TM polarization band gap, and a gap-midgap ra...
基金This work was financially supported by the National Key Research and Development Program of China(2018YFA0703000)the NationalNatural Science Foundation of China(81670972,31872752)+1 种基金Key Research and Development Program of Zhejiang,China(2017C01054,2018C03062,2017C01063)Postdoctoral Science Foundation of China(2020TQ0257,2020M681896).
文摘The organized alignment of cells in various tissues plays a significant role in the maintenance of specific functions.To induce such an alignment,ideal scaffolds should simulate the characteristics and morphologies of natural tissues.Aligned structures that guide cell orientation are used to facilitate tissue regeneration and repair.We here review how various aligned structures are fabricated,including aligned electrospun nanofibers,aligned porous or channeled structures,micropatterns and combinations thereof,and their application in nerve,skeletal muscle,tendon,and tubular dentin regeneration.The future use of aligned structures in tissue engineering is also discussed.
