目的:探讨槲皮素对糖尿病肾病的影响及该影响与肾小球周期素激酶抑制剂P27的关系。方法:腹腔注射链脲佐菌素建立糖尿病(DM)模型,分别以生理盐水或槲皮素(100 mg·kg1·d-1)灌胃8周。蛋白印迹(Western杂交)法测定肾小球裂解液P2...目的:探讨槲皮素对糖尿病肾病的影响及该影响与肾小球周期素激酶抑制剂P27的关系。方法:腹腔注射链脲佐菌素建立糖尿病(DM)模型,分别以生理盐水或槲皮素(100 mg·kg1·d-1)灌胃8周。蛋白印迹(Western杂交)法测定肾小球裂解液P27蛋白水平,ELISA法测定肾小球裂解液细胞外基质(ECM)蛋白(Ⅳ型胶原及纤维连接蛋白)和尿白蛋白。结果:DM 8周大鼠肾小球P27水平增高,ECM蛋白水平增加,尿白蛋白排泄增多,肾质量/体质量比值增高。槲皮素灌胃8周显著降低DM大鼠。肾小球P27水平(10.6±3.1 vs 18.5±4.3,P<0.01)和ECM蛋白水平,减少尿白蛋白排泄[(17.62±3.02)vs(39.62±3.68)μg/24 h,P<0.01],降低DM大鼠肾质量/体质量比值(11.35±1.76 vs 14.87±2.02,P<0.01)。槲皮素不改变DM大鼠血糖水平。结论:槲皮素可能通过降低肾小球P27水平改善糖尿病肾病症状。展开更多
Objective： To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription （祛风通络方, QFTL） on the regulation of mesangial cells （MCs） proliferation and apoptosis. Methods： The MCs used i...Objective： To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription （祛风通络方, QFTL） on the regulation of mesangial cells （MCs） proliferation and apoptosis. Methods： The MCs used in this experiment have undergone five passages induced by lipopolysaccharide （LPS）. Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium （MTT） reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction （qRT-PCR）, respectively. Results： The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively （P〈0.05 or P〈0.01）. Moreover, the inhibitory effect is more significant in the QFTL group at 48 h （P〈0.05）. Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase （P〈0.05 or P〈0.01） to cells at S phase （P〈0.01）, implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group （P〈0.05）, while QFTL and Benazepril serum increased the level of MC apoptosis （P〈0.01）. Moreover, the difference between the QFTL group and the Benazepril group was statistically significant （P〈0.01）. Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 （CDK2） and p21 were significantly increased （P〈0.05 or P〈0.01）, p27 was decreased but with no statistical significance （P〉0.05）; After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly （P〈0.05 or P〈0.01）; Compared with the Benazepril group, QFTL展开更多
文摘目的:探讨槲皮素对糖尿病肾病的影响及该影响与肾小球周期素激酶抑制剂P27的关系。方法:腹腔注射链脲佐菌素建立糖尿病(DM)模型,分别以生理盐水或槲皮素(100 mg·kg1·d-1)灌胃8周。蛋白印迹(Western杂交)法测定肾小球裂解液P27蛋白水平,ELISA法测定肾小球裂解液细胞外基质(ECM)蛋白(Ⅳ型胶原及纤维连接蛋白)和尿白蛋白。结果:DM 8周大鼠肾小球P27水平增高,ECM蛋白水平增加,尿白蛋白排泄增多,肾质量/体质量比值增高。槲皮素灌胃8周显著降低DM大鼠。肾小球P27水平(10.6±3.1 vs 18.5±4.3,P<0.01)和ECM蛋白水平,减少尿白蛋白排泄[(17.62±3.02)vs(39.62±3.68)μg/24 h,P<0.01],降低DM大鼠肾质量/体质量比值(11.35±1.76 vs 14.87±2.02,P<0.01)。槲皮素不改变DM大鼠血糖水平。结论:槲皮素可能通过降低肾小球P27水平改善糖尿病肾病症状。
基金Supported by the National Natural Science Foundation of China(No.30801504,No.30901926,No.81100530 and No.81070590)
文摘Objective： To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription （祛风通络方, QFTL） on the regulation of mesangial cells （MCs） proliferation and apoptosis. Methods： The MCs used in this experiment have undergone five passages induced by lipopolysaccharide （LPS）. Changes in the proliferation, apoptosis, cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium （MTT） reduction assay, flow cytometry, Western blot and quantitative real-time polymerase chain reaction （qRT-PCR）, respectively. Results： The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24, 48 and 72 h, respectively （P〈0.05 or P〈0.01）. Moreover, the inhibitory effect is more significant in the QFTL group at 48 h （P〈0.05）. Compared with the control group, LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases, while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase （P〈0.05 or P〈0.01） to cells at S phase （P〈0.01）, implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis, compared with the control group （P〈0.05）, while QFTL and Benazepril serum increased the level of MC apoptosis （P〈0.01）. Moreover, the difference between the QFTL group and the Benazepril group was statistically significant （P〈0.01）. Compared with the control group, the protein and mRNA expression levels of cylinD1, cyclin dependent kinase 2 （CDK2） and p21 were significantly increased （P〈0.05 or P〈0.01）, p27 was decreased but with no statistical significance （P〉0.05）; After being treated with QFTL and Benazepril serum, the protein and mRNA expression levels of cylinD1, CDK2, p21 were decreased and p27 increased significantly （P〈0.05 or P〈0.01）; Compared with the Benazepril group, QFTL
