Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can r...Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can reduce the frequence which 10 μmol/L 5-HT induced [Ca2+] spark in SFSMC of calcium over loading to 50%, and amplitude to 50% or so. We can draw a conclusion that dihydropyridines cal-cium antagonists lacidipine can antagonize the release of intracellular [Ca2+] which 5-HT-induced in dose dependent manner.展开更多
A new dynamic model for non-Fickian diffu-sion of calcium spark in cardiac myocytes was developed by introducing time lags on the basis of the microscale mass transport theory. Numerical simulation showed that the siz...A new dynamic model for non-Fickian diffu-sion of calcium spark in cardiac myocytes was developed by introducing time lags on the basis of the microscale mass transport theory. Numerical simulation showed that the size of the calcium spark produced by the new dynamic model was larger than that of Fick diffusion and was in more agreement with experimental results. In addition, the time lags of the calcium spark in cardiac myocytes were about 0.1—0.8 ms. These results can be used to understand the mechanism of calcium spark diffusion in cardiac myocytes.展开更多
Calcium release from sarcoplasmic reticulum (SR) though Ryanodine receptors plays an important role in excitation contraction coupling of cardiac myocytes. This
文摘Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can reduce the frequence which 10 μmol/L 5-HT induced [Ca2+] spark in SFSMC of calcium over loading to 50%, and amplitude to 50% or so. We can draw a conclusion that dihydropyridines cal-cium antagonists lacidipine can antagonize the release of intracellular [Ca2+] which 5-HT-induced in dose dependent manner.
基金supported by the National Natural Science Foundation of China(Grant No.10372007)Bio-x center of Peking University and Japan Society for the Promotion of Science(PO2325).
文摘A new dynamic model for non-Fickian diffu-sion of calcium spark in cardiac myocytes was developed by introducing time lags on the basis of the microscale mass transport theory. Numerical simulation showed that the size of the calcium spark produced by the new dynamic model was larger than that of Fick diffusion and was in more agreement with experimental results. In addition, the time lags of the calcium spark in cardiac myocytes were about 0.1—0.8 ms. These results can be used to understand the mechanism of calcium spark diffusion in cardiac myocytes.
文摘Calcium release from sarcoplasmic reticulum (SR) though Ryanodine receptors plays an important role in excitation contraction coupling of cardiac myocytes. This
