To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic se...To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.展开更多
文摘To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.
