目的观察不同压力CO_2气腹处理对结肠癌HT-29细胞体内外增殖与侵袭能力的影响。方法以HT-29细胞暴露于5 mm Hg、10 mm Hg及15 mm Hg CO_2压力下1h建立体外模拟气腹模型为三个实验组,另以常规培养HT-29细胞为对照组。处理1 h后,立即取各...目的观察不同压力CO_2气腹处理对结肠癌HT-29细胞体内外增殖与侵袭能力的影响。方法以HT-29细胞暴露于5 mm Hg、10 mm Hg及15 mm Hg CO_2压力下1h建立体外模拟气腹模型为三个实验组,另以常规培养HT-29细胞为对照组。处理1 h后,立即取各组细胞培养液检测pH值。常规培养24 h后将各组细胞接种裸鼠腹腔建立体内模型(每组对应6只裸鼠),同时体外以四唑盐比色法(methyl thiazolyl tetrazolim,MTT)检测各组细胞粘附率,免疫细胞化学法检测E-钙粘蛋白的表达。继续常规培养5 d,绘制细胞生长曲线。3 w后开腹检查裸鼠腹腔肿瘤种植转移情况并测量肿瘤平均质量,以免疫组化法检测肿瘤组织中E-钙粘蛋白的表达。结果气腹处理1 h后,实验组细胞培养液即时p H值显著低于对照组(均P<0.05)。10 mm Hg组和15mm Hg组与对照组相比,细胞粘附率下降、裸鼠腹腔内肿瘤平均质量减小、E-钙粘蛋白表达水平降低(均P<0.05),5 mm Hg组上述指标与对照组比较,差异均无统计学意义(均P>0.05)。各实验组细胞增殖速度随气腹处理压力的升高呈下降趋势。结论模拟10 mm Hg及15 mm Hg CO_2气腹处理,可降低结肠癌HT-29细胞的体内外的增殖能力,不增加在腹腔的侵袭能力。5 mm Hg CO_2气腹处理对HT-29细胞没有显著影响。展开更多
Global climate change and ongoing plant invasion are the two prominent ecological issues threatening biodiversity world wide.Among invasive species,Lantana camara and Hyptis suaveolens are the two most important invad...Global climate change and ongoing plant invasion are the two prominent ecological issues threatening biodiversity world wide.Among invasive species,Lantana camara and Hyptis suaveolens are the two most important invaders in the dry deciduous forest in India.We monitored the growth of these two invasive species and seedlings of four native dry deciduous species(Acacia catechu,Bauhinia variegata,Dalbergia latifolia and Tectona grandis)under ambient(375–395μmol mol^(-1))and elevated CO_(2)(700–750μmol mol^(-1))to study the differential growth response of invasive and native seedlings.Methods Seedlings of all the species were exposed to ambient and elevated CO_(2).After 60 days of exposure,seedlings were harvested and all the growth-related parameters like plant height;biomass of root,stem and leaves;total seedling biomass;R/S ratio;allocation parameters;net assimilation rate(NAR)and relative growth rate(RGR)were determined.Important Findings Biomass,RGR and NAR of all the species increased under elevated CO_(2)but the increase was higher in invasive species and they formed larger seedlings than natives.Therefore under the CO_(2)-enriched future atmosphere,competitive hierarchies could change and may interfere with the species composition of the invaded area.展开更多
文摘目的观察不同压力CO_2气腹处理对结肠癌HT-29细胞体内外增殖与侵袭能力的影响。方法以HT-29细胞暴露于5 mm Hg、10 mm Hg及15 mm Hg CO_2压力下1h建立体外模拟气腹模型为三个实验组,另以常规培养HT-29细胞为对照组。处理1 h后,立即取各组细胞培养液检测pH值。常规培养24 h后将各组细胞接种裸鼠腹腔建立体内模型(每组对应6只裸鼠),同时体外以四唑盐比色法(methyl thiazolyl tetrazolim,MTT)检测各组细胞粘附率,免疫细胞化学法检测E-钙粘蛋白的表达。继续常规培养5 d,绘制细胞生长曲线。3 w后开腹检查裸鼠腹腔肿瘤种植转移情况并测量肿瘤平均质量,以免疫组化法检测肿瘤组织中E-钙粘蛋白的表达。结果气腹处理1 h后,实验组细胞培养液即时p H值显著低于对照组(均P<0.05)。10 mm Hg组和15mm Hg组与对照组相比,细胞粘附率下降、裸鼠腹腔内肿瘤平均质量减小、E-钙粘蛋白表达水平降低(均P<0.05),5 mm Hg组上述指标与对照组比较,差异均无统计学意义(均P>0.05)。各实验组细胞增殖速度随气腹处理压力的升高呈下降趋势。结论模拟10 mm Hg及15 mm Hg CO_2气腹处理,可降低结肠癌HT-29细胞的体内外的增殖能力,不增加在腹腔的侵袭能力。5 mm Hg CO_2气腹处理对HT-29细胞没有显著影响。
文摘Global climate change and ongoing plant invasion are the two prominent ecological issues threatening biodiversity world wide.Among invasive species,Lantana camara and Hyptis suaveolens are the two most important invaders in the dry deciduous forest in India.We monitored the growth of these two invasive species and seedlings of four native dry deciduous species(Acacia catechu,Bauhinia variegata,Dalbergia latifolia and Tectona grandis)under ambient(375–395μmol mol^(-1))and elevated CO_(2)(700–750μmol mol^(-1))to study the differential growth response of invasive and native seedlings.Methods Seedlings of all the species were exposed to ambient and elevated CO_(2).After 60 days of exposure,seedlings were harvested and all the growth-related parameters like plant height;biomass of root,stem and leaves;total seedling biomass;R/S ratio;allocation parameters;net assimilation rate(NAR)and relative growth rate(RGR)were determined.Important Findings Biomass,RGR and NAR of all the species increased under elevated CO_(2)but the increase was higher in invasive species and they formed larger seedlings than natives.Therefore under the CO_(2)-enriched future atmosphere,competitive hierarchies could change and may interfere with the species composition of the invaded area.
