目的 研究CD1a和CD83阳性树突状细胞(DC)在慢性阻塞性肺疾病(COPD)小鼠肺组织中的分布及意义。方法 将20只C57BL/6小鼠采用随机数字表法分为空气对照组和烟熏COPD组,各10例。空气对照组暴露于空气中;烟熏COPD组使用香烟烟熏法建立COPD...目的 研究CD1a和CD83阳性树突状细胞(DC)在慢性阻塞性肺疾病(COPD)小鼠肺组织中的分布及意义。方法 将20只C57BL/6小鼠采用随机数字表法分为空气对照组和烟熏COPD组,各10例。空气对照组暴露于空气中;烟熏COPD组使用香烟烟熏法建立COPD小鼠模型,于最后一次烟熏结束24 h内处死小鼠,取右下肺。观察2组小鼠体质量变化、肺组织病理变化,测量平均内衬间隔(MLI),免疫组化法检测肺组织中CD1a^(+)、CD83^(+)DC的分布并计数。结果 建模7、14、21、28 d时烟熏COPD组的小鼠体质量较空气对照组均降低(P<0.05);HE染色示烟熏COPD组小鼠肺组织正常肺泡结构破坏,多个肺泡相互融合形成较大的肺泡腔,肺泡间隔有大量炎性细胞浸润,肺泡壁增厚,COPD造模成功;与空气对照组比较,烟熏COPD组MLI值(μm)增大(28.30±3.47 vs. 50.40±3.60),肺组织中CD1a^(+)DC数量(个/视野)增多(9.58±2.18 vs. 17.08±3.67),而CD83^(+)DC数量(个/视野)减少(19.78±4.95 vs. 8.02±3.30),差异有统计学意义(均P<0.05)。结论 烟熏COPD组小鼠肺组织CD1a^(+)DC增多,CD83^(+)DC减少,香烟烟熏可能导致DC成熟障碍。展开更多
γδT cells are a kind of innate immune T cell.They have not attracted sufficient attention because they account for only a small proportion of all immune cells,and many basic factors related to these cells remain unc...γδT cells are a kind of innate immune T cell.They have not attracted sufficient attention because they account for only a small proportion of all immune cells,and many basic factors related to these cells remain unclear.However,in recent years,with the rapid development of tumor immunotherapy,γδT cells have attracted increasing attention because of their ability to exert cytotoxic effects on most tumor cells without major histocompatibility complex(MHC)restriction.An increasing number of basic studies have focused on the development,antigen recognition,activation,and antitumor immune response ofγδT cells.Additionally,γδT cell-based immunotherapeutic strategies are being developed,and the number of clinical trials investigating such strategies is increasing.This review mainly summarizes the progress of basic research and the clinical application ofγδT cells in tumor immunotherapy to provide a theoretical basis for further the development ofγδT cell-based strategies in the future.展开更多
文摘目的 研究CD1a和CD83阳性树突状细胞(DC)在慢性阻塞性肺疾病(COPD)小鼠肺组织中的分布及意义。方法 将20只C57BL/6小鼠采用随机数字表法分为空气对照组和烟熏COPD组,各10例。空气对照组暴露于空气中;烟熏COPD组使用香烟烟熏法建立COPD小鼠模型,于最后一次烟熏结束24 h内处死小鼠,取右下肺。观察2组小鼠体质量变化、肺组织病理变化,测量平均内衬间隔(MLI),免疫组化法检测肺组织中CD1a^(+)、CD83^(+)DC的分布并计数。结果 建模7、14、21、28 d时烟熏COPD组的小鼠体质量较空气对照组均降低(P<0.05);HE染色示烟熏COPD组小鼠肺组织正常肺泡结构破坏,多个肺泡相互融合形成较大的肺泡腔,肺泡间隔有大量炎性细胞浸润,肺泡壁增厚,COPD造模成功;与空气对照组比较,烟熏COPD组MLI值(μm)增大(28.30±3.47 vs. 50.40±3.60),肺组织中CD1a^(+)DC数量(个/视野)增多(9.58±2.18 vs. 17.08±3.67),而CD83^(+)DC数量(个/视野)减少(19.78±4.95 vs. 8.02±3.30),差异有统计学意义(均P<0.05)。结论 烟熏COPD组小鼠肺组织CD1a^(+)DC增多,CD83^(+)DC减少,香烟烟熏可能导致DC成熟障碍。
基金supported by grants from the National Natural Science Foundation of China(Nos.32270915,31970843,82071791,U20A20374,and 81972886)National Key Research and Development Program of China(No.2022YFC3602004)+4 种基金CAMS Initiative for Innovative Medicine(Nos.2021-I2M-1-005,2021-I2M-1-035,and 2021-I2M-1-053)Haihe Laboratory of Cell Ecosystem Innovation Fund(No.22HHXBSS00028)CAMS Central Public Welfare Scientific Research Institute Basal Research Expenses(No.3332020035)Changzhou Science and Technology Support Plan(No.CE20215008)Beijing Municipal Commission of Science and Technology Fund for Innovative Drug(No.Z221100007922040)
文摘γδT cells are a kind of innate immune T cell.They have not attracted sufficient attention because they account for only a small proportion of all immune cells,and many basic factors related to these cells remain unclear.However,in recent years,with the rapid development of tumor immunotherapy,γδT cells have attracted increasing attention because of their ability to exert cytotoxic effects on most tumor cells without major histocompatibility complex(MHC)restriction.An increasing number of basic studies have focused on the development,antigen recognition,activation,and antitumor immune response ofγδT cells.Additionally,γδT cell-based immunotherapeutic strategies are being developed,and the number of clinical trials investigating such strategies is increasing.This review mainly summarizes the progress of basic research and the clinical application ofγδT cells in tumor immunotherapy to provide a theoretical basis for further the development ofγδT cell-based strategies in the future.
