The aim of this paper is to present the main results arisen from the joint efforts of the public-private partnership in being between CCII （Centro Cultura Innovativa d＇Impresa） of University of Salento and Apphia s...The aim of this paper is to present the main results arisen from the joint efforts of the public-private partnership in being between CCII （Centro Cultura Innovativa d＇Impresa） of University of Salento and Apphia s.r.l.. This paper will focus on the results of the core of the OBSS （on board stability system） module that, starting from the exact 3D geometry of a ship hull, described by a discrete model in a standard format, the distribution of all weights onboard and the data acquired by the system, calculates ship floating conditions （in draught, heel and trim）. The proposed approach is based on geometry meshes represented by an universally accepted file format （named OBJ） first developed by Wavefront Technologies. Demonstration of the validity of the results is done by the authors using as hull model a simple rectangular box.展开更多
Two new current mode active-RC networks using the second generation current conveyer (CCII) devices are presented. The circuits provide high-Q bandpass (BP)/lowpass(LP) filter characteristics; the highpass(HP) respons...Two new current mode active-RC networks using the second generation current conveyer (CCII) devices are presented. The circuits provide high-Q bandpass (BP)/lowpass(LP) filter characteristics; the highpass(HP) response may also be obtained with suitable design. Current mode sine wave signal generation (Q→α) is possible by tuning a grounded resistor. With non-ideal CCIIs the design equations are slightly altered owing to CCII port current and voltage tracking errors (Ei,v).展开更多
Effective treatment of rheumatoid arthritis can be mediated by native chicken type II collagen(n CCII), recombinant peptide containing n CCII tolerogenic epitopes(CTEs), or a therapeutic DNA vaccine encoding the full-...Effective treatment of rheumatoid arthritis can be mediated by native chicken type II collagen(n CCII), recombinant peptide containing n CCII tolerogenic epitopes(CTEs), or a therapeutic DNA vaccine encoding the full-length CCOL2 A1 c DNA. As recombinant CCII(r CCII) might avoid potential pathogenic virus contamination during n CCII preparation or chromosomal integration and oncogene activation associated with DNA vaccines, here we evaluated the importance of propeptide and telopeptide domains on r CCII triple helix molecular assembly. We constructed p C-and p N-procollagen(without N-or Cpropeptides, respectively) as well as CTEs located in the triple helical domain lacking both propeptides and telopeptides, and expressed these in yeast Pichia pastoris host strain GS115(his4, Mut+) simultaneously with recombinant chicken prolyl-4-hydroxylase α and β subunits. Both p C-and p N-procollagen monomers accumulated inside P. pastoris cells, whereas CTE was assembled into homotrimers with stable conformation and secreted into the supernatants, suggesting that the large molecular weight p C-or p N-procollagens were retained within the endoplasmic reticulum whereas the smaller CTEs proceeded through the secretory pathway. Furthermore, resulting recombinant chicken type II collagen p Cα1(II) can induce collagen-induced arthritis(CIA) rat model, which seems to be as effective as the current standard n CCII. Notably, protease digestion assays showed that r CCII could assemble in the absence of C-and N-propeptides or telopeptides. These findings provide new insights into the minimal structural requirements for r CCII expression and folding.展开更多
文摘The aim of this paper is to present the main results arisen from the joint efforts of the public-private partnership in being between CCII （Centro Cultura Innovativa d＇Impresa） of University of Salento and Apphia s.r.l.. This paper will focus on the results of the core of the OBSS （on board stability system） module that, starting from the exact 3D geometry of a ship hull, described by a discrete model in a standard format, the distribution of all weights onboard and the data acquired by the system, calculates ship floating conditions （in draught, heel and trim）. The proposed approach is based on geometry meshes represented by an universally accepted file format （named OBJ） first developed by Wavefront Technologies. Demonstration of the validity of the results is done by the authors using as hull model a simple rectangular box.
文摘Two new current mode active-RC networks using the second generation current conveyer (CCII) devices are presented. The circuits provide high-Q bandpass (BP)/lowpass(LP) filter characteristics; the highpass(HP) response may also be obtained with suitable design. Current mode sine wave signal generation (Q→α) is possible by tuning a grounded resistor. With non-ideal CCIIs the design equations are slightly altered owing to CCII port current and voltage tracking errors (Ei,v).
文摘Effective treatment of rheumatoid arthritis can be mediated by native chicken type II collagen(n CCII), recombinant peptide containing n CCII tolerogenic epitopes(CTEs), or a therapeutic DNA vaccine encoding the full-length CCOL2 A1 c DNA. As recombinant CCII(r CCII) might avoid potential pathogenic virus contamination during n CCII preparation or chromosomal integration and oncogene activation associated with DNA vaccines, here we evaluated the importance of propeptide and telopeptide domains on r CCII triple helix molecular assembly. We constructed p C-and p N-procollagen(without N-or Cpropeptides, respectively) as well as CTEs located in the triple helical domain lacking both propeptides and telopeptides, and expressed these in yeast Pichia pastoris host strain GS115(his4, Mut+) simultaneously with recombinant chicken prolyl-4-hydroxylase α and β subunits. Both p C-and p N-procollagen monomers accumulated inside P. pastoris cells, whereas CTE was assembled into homotrimers with stable conformation and secreted into the supernatants, suggesting that the large molecular weight p C-or p N-procollagens were retained within the endoplasmic reticulum whereas the smaller CTEs proceeded through the secretory pathway. Furthermore, resulting recombinant chicken type II collagen p Cα1(II) can induce collagen-induced arthritis(CIA) rat model, which seems to be as effective as the current standard n CCII. Notably, protease digestion assays showed that r CCII could assemble in the absence of C-and N-propeptides or telopeptides. These findings provide new insights into the minimal structural requirements for r CCII expression and folding.
