目的通过对慢性鼻一鼻窦炎伴鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）患者肺功能的检测，分析鼻息肉的病变范围及炎症状态与下呼吸道病变发生的相关性及临床意义。方法对2011年3月至6月住院手术的56例CRSwNP患者进...目的通过对慢性鼻一鼻窦炎伴鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）患者肺功能的检测，分析鼻息肉的病变范围及炎症状态与下呼吸道病变发生的相关性及临床意义。方法对2011年3月至6月住院手术的56例CRSwNP患者进行前瞻性研究，详细询问病史，术前均行变应原皮肤点刺试验、肺功能检测、鼻内镜检查、鼻窦CT检查、术后鼻息肉组织嗜酸性粒细胞计数。依据以上检测结果将患者分组，比较各组间气道高反应性、哮喘、肺功能异常的发生率及其与鼻息肉病变范围和炎症状态的相关性。结果CRSwNP患者发生气道高反应性、哮喘、肺功能异常的比率分别为37．5％、44．6％、53．6％；没有明显下呼吸道症状患者肺功能异常的发生率亦高达50．0％。CRSwNP患者鼻内镜评分、CT评分分值越高，其发生气道高反应性、哮喘、肺功能异常的比率越大，组问差异均有统计学意义（鼻内镜评分）（苔镜值分别为8．077、3．989、7．445，P值〈0．01或〈0．05；x2cT值分别为3．863、5．380、4．309；4．293、4．293、4．572；10．572、13．504、13．295；P值〈0．01或〈0．05）。皮肤点刺实验阳性患者发生气道高反应性和哮喘的比率比阴性患者大，组间差异有统计学意义（P值〈0．05）。鼻息肉组织中嗜酸性粒细胞增多患者气道高反应、哮喘、肺功能异常的发生率均明显高于嗜酸性粒细胞正常的患者，组间差异有统计学意义（P值〈0．05或〈0．01）。结论CRSwNP患者可能伴发有症状或无症状的下呼吸道病变，病变范围及炎症程度与下呼吸道病变的发生率呈正相关性。展开更多
目的探讨组蛋白脱乙酰基酶6（HDAC6）抑制剂Tubastatin A Hcl对急性支气管哮喘（简称哮喘）小鼠气管炎症的干预作用。方法48只BALB／C小鼠按随机数字表法随机分为正常组、哮喘组、地塞米松组和Tubastatin A Hcl组各12只。测定各组小鼠...目的探讨组蛋白脱乙酰基酶6（HDAC6）抑制剂Tubastatin A Hcl对急性支气管哮喘（简称哮喘）小鼠气管炎症的干预作用。方法48只BALB／C小鼠按随机数字表法随机分为正常组、哮喘组、地塞米松组和Tubastatin A Hcl组各12只。测定各组小鼠气管反应性，计数其支气管肺泡灌洗液（BALF）中细胞总数和分类细胞数以及白细胞介素（IL）-4、IL-5和γ干扰素（IFN-γ）水平。取各组小鼠肺组织分别通过HE染色观察气管炎症浸润、AB-PAS染色观察气管上皮杯状细胞化生、Masson染色观察肺组织胶原沉积情况。结果Tubastatin A Hcl组气管反应性显著低于哮喘组[（4．18±0．94）比（6．02±0．47），P〈0．05]；Tubastatin A Hcl组BALF中炎症细胞总数[（57．0±5．7）×10^4／ml比（87．0±5．6）×10^4／m1]、嗜酸性粒细胞数[（6．8±1．7）×10^4／ml比（12．3±3．5）×10^4／m1]、IL-4[（19．3±2．7）比（26．2±3．2）ng／ml]水平均显著低于哮喘组（均P〈0．05），IL-5低于哮喘组、IFN-γ水平高于哮喘组但差异均无统计学意义（均P〉0．05）；Tubastatin A Hcl组肺组织气管血管周围炎症细胞浸润程度、炎症细胞数[（9．80±2．42）比（20．67±7．53）个]、炎症评分[（2．20±0．70）比（3．60±0．68）分]、杯状细胞化生百分比[（50．46±5．03）％比（71．06±5．38）％]均显著低于哮喘组（均P〈0．05），胶原沉积面积低于哮喘组但差异无统计学意义（P〉0．05）。但上述结果地塞米松组均略优于TubastatinAHcl组但差异均无统计学意义（均P〉0．05）。结论Tubastatin A Hcl能够有效缓解急性期哮喘气管炎症水平，但其抗炎作用有限，效果并不如地塞米松显著。展开更多
Asthma is one of the most common chronic respiratory pathologies worldwide. The cost of its treatment, particularly for populations in developing countries like Senegal, requires an alternative which consists of devel...Asthma is one of the most common chronic respiratory pathologies worldwide. The cost of its treatment, particularly for populations in developing countries like Senegal, requires an alternative which consists of developing research into new therapeutic approaches with the use of plants from traditional pharmacopoeia. The objective of this study was to determine whether the hydroalcoholic extract of Terminalia avicennioides leaves has a preventive effect on the constriction of tracheal smooth muscle induced by Acetylcholine in Wistar strain rats. Methods: The species used in our experiments were male Wistar rats aged between 12 and 14 weeks. Rings of rat trachea were suspended in isolated organ chambers connected to tension sensors. Two pharmacological agents were used: acetylcholine, for its constrictor effect and adrenaline, for its dilating effect on the pulmonary trachea. Our plant material consisted of a hydroalcoholic extract of Terminalia avicennioides leaves. The experimental protocol was that of the preventive model which consisted of impregnating these rings with the extract at a defined concentration then testing its contractility in the presence of Acetylcholine which was the contractile agonist used in this study. The preventive effect of the extract was studied with an Acetylcholine contraction range on rings pre-incubated with Terminalia avicennioides extract. Results: The results showed that Acetylcholine contracts the trachea and the increase in its contractility was dependent on the dose of Ach added cumulatively (10<sup>−5</sup> to 4 × 10<sup>−5</sup> mol/l). The administration of the hydroalcoholic extract of Terminalia avicennioides leaves led to a modification of the contractile response to Acetylcholine. Indeed, this extract induced a significant attenuation of the contractile responses of the treated tracheal rings compared to those untreated. Conclusion: Terminalia avicennioides had a relaxing effect on tracheal smooth muscle and this effect could play an important role in the prevent展开更多
文摘目的通过对慢性鼻一鼻窦炎伴鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）患者肺功能的检测，分析鼻息肉的病变范围及炎症状态与下呼吸道病变发生的相关性及临床意义。方法对2011年3月至6月住院手术的56例CRSwNP患者进行前瞻性研究，详细询问病史，术前均行变应原皮肤点刺试验、肺功能检测、鼻内镜检查、鼻窦CT检查、术后鼻息肉组织嗜酸性粒细胞计数。依据以上检测结果将患者分组，比较各组间气道高反应性、哮喘、肺功能异常的发生率及其与鼻息肉病变范围和炎症状态的相关性。结果CRSwNP患者发生气道高反应性、哮喘、肺功能异常的比率分别为37．5％、44．6％、53．6％；没有明显下呼吸道症状患者肺功能异常的发生率亦高达50．0％。CRSwNP患者鼻内镜评分、CT评分分值越高，其发生气道高反应性、哮喘、肺功能异常的比率越大，组问差异均有统计学意义（鼻内镜评分）（苔镜值分别为8．077、3．989、7．445，P值〈0．01或〈0．05；x2cT值分别为3．863、5．380、4．309；4．293、4．293、4．572；10．572、13．504、13．295；P值〈0．01或〈0．05）。皮肤点刺实验阳性患者发生气道高反应性和哮喘的比率比阴性患者大，组间差异有统计学意义（P值〈0．05）。鼻息肉组织中嗜酸性粒细胞增多患者气道高反应、哮喘、肺功能异常的发生率均明显高于嗜酸性粒细胞正常的患者，组间差异有统计学意义（P值〈0．05或〈0．01）。结论CRSwNP患者可能伴发有症状或无症状的下呼吸道病变，病变范围及炎症程度与下呼吸道病变的发生率呈正相关性。
文摘目的探讨组蛋白脱乙酰基酶6（HDAC6）抑制剂Tubastatin A Hcl对急性支气管哮喘（简称哮喘）小鼠气管炎症的干预作用。方法48只BALB／C小鼠按随机数字表法随机分为正常组、哮喘组、地塞米松组和Tubastatin A Hcl组各12只。测定各组小鼠气管反应性，计数其支气管肺泡灌洗液（BALF）中细胞总数和分类细胞数以及白细胞介素（IL）-4、IL-5和γ干扰素（IFN-γ）水平。取各组小鼠肺组织分别通过HE染色观察气管炎症浸润、AB-PAS染色观察气管上皮杯状细胞化生、Masson染色观察肺组织胶原沉积情况。结果Tubastatin A Hcl组气管反应性显著低于哮喘组[（4．18±0．94）比（6．02±0．47），P〈0．05]；Tubastatin A Hcl组BALF中炎症细胞总数[（57．0±5．7）×10^4／ml比（87．0±5．6）×10^4／m1]、嗜酸性粒细胞数[（6．8±1．7）×10^4／ml比（12．3±3．5）×10^4／m1]、IL-4[（19．3±2．7）比（26．2±3．2）ng／ml]水平均显著低于哮喘组（均P〈0．05），IL-5低于哮喘组、IFN-γ水平高于哮喘组但差异均无统计学意义（均P〉0．05）；Tubastatin A Hcl组肺组织气管血管周围炎症细胞浸润程度、炎症细胞数[（9．80±2．42）比（20．67±7．53）个]、炎症评分[（2．20±0．70）比（3．60±0．68）分]、杯状细胞化生百分比[（50．46±5．03）％比（71．06±5．38）％]均显著低于哮喘组（均P〈0．05），胶原沉积面积低于哮喘组但差异无统计学意义（P〉0．05）。但上述结果地塞米松组均略优于TubastatinAHcl组但差异均无统计学意义（均P〉0．05）。结论Tubastatin A Hcl能够有效缓解急性期哮喘气管炎症水平，但其抗炎作用有限，效果并不如地塞米松显著。
文摘Asthma is one of the most common chronic respiratory pathologies worldwide. The cost of its treatment, particularly for populations in developing countries like Senegal, requires an alternative which consists of developing research into new therapeutic approaches with the use of plants from traditional pharmacopoeia. The objective of this study was to determine whether the hydroalcoholic extract of Terminalia avicennioides leaves has a preventive effect on the constriction of tracheal smooth muscle induced by Acetylcholine in Wistar strain rats. Methods: The species used in our experiments were male Wistar rats aged between 12 and 14 weeks. Rings of rat trachea were suspended in isolated organ chambers connected to tension sensors. Two pharmacological agents were used: acetylcholine, for its constrictor effect and adrenaline, for its dilating effect on the pulmonary trachea. Our plant material consisted of a hydroalcoholic extract of Terminalia avicennioides leaves. The experimental protocol was that of the preventive model which consisted of impregnating these rings with the extract at a defined concentration then testing its contractility in the presence of Acetylcholine which was the contractile agonist used in this study. The preventive effect of the extract was studied with an Acetylcholine contraction range on rings pre-incubated with Terminalia avicennioides extract. Results: The results showed that Acetylcholine contracts the trachea and the increase in its contractility was dependent on the dose of Ach added cumulatively (10<sup>−5</sup> to 4 × 10<sup>−5</sup> mol/l). The administration of the hydroalcoholic extract of Terminalia avicennioides leaves led to a modification of the contractile response to Acetylcholine. Indeed, this extract induced a significant attenuation of the contractile responses of the treated tracheal rings compared to those untreated. Conclusion: Terminalia avicennioides had a relaxing effect on tracheal smooth muscle and this effect could play an important role in the prevent
