In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH gro...In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH group in MCQ with a common sensing unit, 2,4-dinitrobezensulfonate group(DNBS) for biothiols. Due to the photo-induced electron transfer(PET) process between MCQ and DNBS, this probe was weekly fluorescent. Upon the addition of biothiols(Cys, Hcy and GSH), this probe emitted a strong red fluorescence(λ_(em max)=613 nm) with a large Stokes shift(115 nm). In addition,fluorescence imaging of biothiols in living cells was successfully realized using MCQ-DNBS as a detector.展开更多
A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）,...A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）, pyridyl-functionalized poly（phenylene ethynylene）（Pl）. The fluorescence of P1 can be significantly quenched by Ag＋ due to complexation-mediated interpolymer aggregation. Furthermore, biothiols can efficiently recover the fluorescence intensity of P1 as a result of the stronger binding between thiol group and Ag＋, which dissociates PI from the P1/Ag＋ complex and disrupts interpolymer aggregation. Under optimum conditions, a good linear relation- ship in a range of 100--4200 nmol/L is obtained for GSH with a detection limit of 80 nmol/L（S/N=3）. As a result of specific interaction between the thiol group and Ag＋, the proposed method shows a high selectivity for biothiols. In addition, the CPE-based fluorescence ＂off-on＂ switch has been used to quantitatively detect total biothiols in cell lysates.展开更多
Nano material based drug delivery system have received great attention in clinical application due to their high therapeutic efficacy and lower side effects than classical method,multi-functional nanomaterial also hav...Nano material based drug delivery system have received great attention in clinical application due to their high therapeutic efficacy and lower side effects than classical method,multi-functional nanomaterial also have shown the excellent performance at cancer theranostic and durg tracking in vivo and in vitro.However,most of these works are influenced by the bio-toxicity of applied nanomaterials,which could influence the diagnostic results and treatment effect.Therefore,we have prepared a high biocompatibility porous carbon nanospheres(PCNs) based nano-system(PCN-siRNA-DOX-FA) for targeted drug delivery and the ranostic.The surface modifications have increased dispersion and stability of the PCNs,and folic acid(FA) had enhanced the active target ability for FA receptor positive cell lines.Moreover,through the siRNA structure and doxorubicin(DOX) loading,biological and chemical combined multi-therapy was achieved in cancerous cells.This constructed nano-system could positively improve the biotoxicity problem of nanomaterial and provide a potential platform for clinical cancer theranostic applications.展开更多
N-Acety-L-cysteine(NAC),enriched with multiple electron-rich groups involving-SH,-C=O,and-COOH,exhibits strong affinities to transition metal ions.Herein,NAC was adopted as the stabilizing agent to synthesize a series...N-Acety-L-cysteine(NAC),enriched with multiple electron-rich groups involving-SH,-C=O,and-COOH,exhibits strong affinities to transition metal ions.Herein,NAC was adopted as the stabilizing agent to synthesize a series of peroxidase-like Pt nanoclusters(NCs)with different physicochemical properties.Both the cluster size and the charge state of NAC-Pt NCs are highly dependent upon the molar ratio of[K_(2)PtCl_(4)]/[NAC],contributing to different peroxidase mimicking activities.The most active Pt nanozyme(~1.7 nm),composed of 60%metallic Pt^(0) and 40%positively charged Pt^(2+)respectively,exhibits the K_(m) of 0.132 mM for 3,3′,5,5′-tetramethylbenzidine and 35 mM for H_(2)O_(2).Based on the heparin-activated enzymatic activ-ity of NAC-Pt at pH 6.0,an ultrasensitive test was established for quantitative determination of heparin,giving the linear response of 0.5-20μg/mL as well as the limit of detection of 2×10^(−3)μg/mL.This proposed method is also applicable in biological fluid for practical applications.Such a colorimetric method possesses several advantages involving high sensitiv-ity,short response,eco-friendly synthesis,low consumption of material and energy,free of expensive instrument,as well as simple operation.展开更多
基金supported by the National Natural Science Foundation of China (No.U1608222)Special Fund for Agroscientific Research in the Public Interest of China(No.201503108)+1 种基金Fundamental Research Funds for the Central Universities of Central South University (No. 2018zzts109)the State Key Laboratory of Chemo/Biosensing and Chemometrics (No. 2016005)
文摘In this work, we presented a fluorescent probe(MCQ-DNBS) for selective and sensitive detection of biothiols based on a methylated chromenoquinoline(MCQ) derivative. Probe MCQ-DNBS was constructed by masking the OH group in MCQ with a common sensing unit, 2,4-dinitrobezensulfonate group(DNBS) for biothiols. Due to the photo-induced electron transfer(PET) process between MCQ and DNBS, this probe was weekly fluorescent. Upon the addition of biothiols(Cys, Hcy and GSH), this probe emitted a strong red fluorescence(λ_(em max)=613 nm) with a large Stokes shift(115 nm). In addition,fluorescence imaging of biothiols in living cells was successfully realized using MCQ-DNBS as a detector.
基金Supported by the National Natural Science Foundation of China（No.20805014）, the Scientific Research Fund of Hunan Pro- vincial Education Department, China（Nos. 11 C0809 and 12B077）, the Open Fund Project of Key Laboratory in Hunan University, China（No.201103） and the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province, China.
文摘A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）, pyridyl-functionalized poly（phenylene ethynylene）（Pl）. The fluorescence of P1 can be significantly quenched by Ag＋ due to complexation-mediated interpolymer aggregation. Furthermore, biothiols can efficiently recover the fluorescence intensity of P1 as a result of the stronger binding between thiol group and Ag＋, which dissociates PI from the P1/Ag＋ complex and disrupts interpolymer aggregation. Under optimum conditions, a good linear relation- ship in a range of 100--4200 nmol/L is obtained for GSH with a detection limit of 80 nmol/L（S/N=3）. As a result of specific interaction between the thiol group and Ag＋, the proposed method shows a high selectivity for biothiols. In addition, the CPE-based fluorescence ＂off-on＂ switch has been used to quantitatively detect total biothiols in cell lysates.
基金support from the Project Fund for Shandong Key R&D Program (No.2017GGX20121)
文摘Nano material based drug delivery system have received great attention in clinical application due to their high therapeutic efficacy and lower side effects than classical method,multi-functional nanomaterial also have shown the excellent performance at cancer theranostic and durg tracking in vivo and in vitro.However,most of these works are influenced by the bio-toxicity of applied nanomaterials,which could influence the diagnostic results and treatment effect.Therefore,we have prepared a high biocompatibility porous carbon nanospheres(PCNs) based nano-system(PCN-siRNA-DOX-FA) for targeted drug delivery and the ranostic.The surface modifications have increased dispersion and stability of the PCNs,and folic acid(FA) had enhanced the active target ability for FA receptor positive cell lines.Moreover,through the siRNA structure and doxorubicin(DOX) loading,biological and chemical combined multi-therapy was achieved in cancerous cells.This constructed nano-system could positively improve the biotoxicity problem of nanomaterial and provide a potential platform for clinical cancer theranostic applications.
基金This study was funded by National Natural Science Foundation of China(21878225,21776215).
文摘N-Acety-L-cysteine(NAC),enriched with multiple electron-rich groups involving-SH,-C=O,and-COOH,exhibits strong affinities to transition metal ions.Herein,NAC was adopted as the stabilizing agent to synthesize a series of peroxidase-like Pt nanoclusters(NCs)with different physicochemical properties.Both the cluster size and the charge state of NAC-Pt NCs are highly dependent upon the molar ratio of[K_(2)PtCl_(4)]/[NAC],contributing to different peroxidase mimicking activities.The most active Pt nanozyme(~1.7 nm),composed of 60%metallic Pt^(0) and 40%positively charged Pt^(2+)respectively,exhibits the K_(m) of 0.132 mM for 3,3′,5,5′-tetramethylbenzidine and 35 mM for H_(2)O_(2).Based on the heparin-activated enzymatic activ-ity of NAC-Pt at pH 6.0,an ultrasensitive test was established for quantitative determination of heparin,giving the linear response of 0.5-20μg/mL as well as the limit of detection of 2×10^(−3)μg/mL.This proposed method is also applicable in biological fluid for practical applications.Such a colorimetric method possesses several advantages involving high sensitiv-ity,short response,eco-friendly synthesis,low consumption of material and energy,free of expensive instrument,as well as simple operation.
