目的:观察胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石中的华支睾吸虫卵形态和存活状况,探讨华支睾吸虫感染与胆囊结石的关系.方法:选取20例胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石标本,行光学显微镜、扫描电...目的:观察胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石中的华支睾吸虫卵形态和存活状况,探讨华支睾吸虫感染与胆囊结石的关系.方法:选取20例胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石标本,行光学显微镜、扫描电镜(scanning electron microscope,SEM)观察其中的华支睾吸虫虫卵形态;进一步用Von Kossa染色和X射线能谱仪分析虫卵表面及其周围物质的钙盐含量、台盼蓝(Trypan blue)染色观察虫卵的存活情况.结果:(1)胆汁中88.3%的虫卵具有典型形态,而胆泥中56.7%的虫卵和结石中91.5%的虫卵出现变形(包括外形、大小改变,卵盖丢失、卵内毛蚴看不清、表面或四周吸附胆红素);电镜下,卵壳为凹凸不平的纹理状结构,表面和周围不同程度黏附有黏液样物质;(2)从胆汁、胆泥到结石,虫卵表面及其周围物质的钙盐含量逐步增多;(3)胆汁、胆泥和结石中虫卵的存活率分别是86.6%、42.1%和11.5%.结论:人体感染华支睾吸虫后,虫卵可通过卵壳的纹理状结构和胆道的黏液样物质储留于胆囊内并发生变形、变性、死亡以至钙化成核,形成胆泥,进而参与形成胆囊结石.展开更多
Endoscopic ultrasonography(EUS) is considered a superior investigation when compared to conventional ultrasonography for imaging gall bladder(GB) lesions as it can provide high-resolution images of small lesions with ...Endoscopic ultrasonography(EUS) is considered a superior investigation when compared to conventional ultrasonography for imaging gall bladder(GB) lesions as it can provide high-resolution images of small lesions with higher ultrasound frequencies. Examination of GB is frequently the primary indication of EUS imaging. Imaging during EUS may not remain restricted to one station and multi-station imaging may provide useful information. This review describes the techniques of imaging of GB by linear EUS from three different stations. The basic difference of imaging between the three stations is that effective imaging from station 1 is done above the neck of GB, from station 2 at the level of the neck of GB and from station 3 below the level of the neck of GB.展开更多
文摘目的:观察胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石中的华支睾吸虫卵形态和存活状况,探讨华支睾吸虫感染与胆囊结石的关系.方法:选取20例胆囊结石合并华支睾吸虫感染患者的胆囊胆汁、胆泥及结石标本,行光学显微镜、扫描电镜(scanning electron microscope,SEM)观察其中的华支睾吸虫虫卵形态;进一步用Von Kossa染色和X射线能谱仪分析虫卵表面及其周围物质的钙盐含量、台盼蓝(Trypan blue)染色观察虫卵的存活情况.结果:(1)胆汁中88.3%的虫卵具有典型形态,而胆泥中56.7%的虫卵和结石中91.5%的虫卵出现变形(包括外形、大小改变,卵盖丢失、卵内毛蚴看不清、表面或四周吸附胆红素);电镜下,卵壳为凹凸不平的纹理状结构,表面和周围不同程度黏附有黏液样物质;(2)从胆汁、胆泥到结石,虫卵表面及其周围物质的钙盐含量逐步增多;(3)胆汁、胆泥和结石中虫卵的存活率分别是86.6%、42.1%和11.5%.结论:人体感染华支睾吸虫后,虫卵可通过卵壳的纹理状结构和胆道的黏液样物质储留于胆囊内并发生变形、变性、死亡以至钙化成核,形成胆泥,进而参与形成胆囊结石.
文摘Endoscopic ultrasonography(EUS) is considered a superior investigation when compared to conventional ultrasonography for imaging gall bladder(GB) lesions as it can provide high-resolution images of small lesions with higher ultrasound frequencies. Examination of GB is frequently the primary indication of EUS imaging. Imaging during EUS may not remain restricted to one station and multi-station imaging may provide useful information. This review describes the techniques of imaging of GB by linear EUS from three different stations. The basic difference of imaging between the three stations is that effective imaging from station 1 is done above the neck of GB, from station 2 at the level of the neck of GB and from station 3 below the level of the neck of GB.
