细胞凋亡(apoptosis),又称程序性细胞死亡(programmed cell death),是一种进化保守和遗传决定的细胞自杀,其性质为生理性。在多细胞动物,凋亡对正常机体的发育和自身稳定起着极其重要的作用。现已发现,有许多疾病的发生和发展与凋亡机...细胞凋亡(apoptosis),又称程序性细胞死亡(programmed cell death),是一种进化保守和遗传决定的细胞自杀,其性质为生理性。在多细胞动物,凋亡对正常机体的发育和自身稳定起着极其重要的作用。现已发现,有许多疾病的发生和发展与凋亡机制异常有关。细胞凋亡为生物医学领域中最热门的课题之一。在过去5年里对细胞凋亡的分子机制的研究取得了突破性进展。现已证明,多种基因产物参与了细胞凋亡过程,其中包括肿瘤坏死因子受体(TNFR)基因超家族的产物死亡受体(death receptors)、连接蛋白(adapters)、天门冬氨酸特异性半胱氨酸蛋白酶(caspases)和Bcl-2家族调节蛋白(Bcl-2 protein family regulators)等。目前对每组蛋白的成员的化学特性、基本功能和它们的上游和下游的相互作用分子等均有了较清楚的了解。这些结果为发展以凋亡机制相关蛋白为靶分子的治疗性细胞凋亡干预手段奠定了基础。展开更多
Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin agai...Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.展开更多
文摘细胞凋亡(apoptosis),又称程序性细胞死亡(programmed cell death),是一种进化保守和遗传决定的细胞自杀,其性质为生理性。在多细胞动物,凋亡对正常机体的发育和自身稳定起着极其重要的作用。现已发现,有许多疾病的发生和发展与凋亡机制异常有关。细胞凋亡为生物医学领域中最热门的课题之一。在过去5年里对细胞凋亡的分子机制的研究取得了突破性进展。现已证明,多种基因产物参与了细胞凋亡过程,其中包括肿瘤坏死因子受体(TNFR)基因超家族的产物死亡受体(death receptors)、连接蛋白(adapters)、天门冬氨酸特异性半胱氨酸蛋白酶(caspases)和Bcl-2家族调节蛋白(Bcl-2 protein family regulators)等。目前对每组蛋白的成员的化学特性、基本功能和它们的上游和下游的相互作用分子等均有了较清楚的了解。这些结果为发展以凋亡机制相关蛋白为靶分子的治疗性细胞凋亡干预手段奠定了基础。
基金supported by the National Natural Science Foundation of China(No.81173065)
文摘Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.
