Basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently deco...Basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently decolorizing dye solutions. Many mushrooms also produce lectins, a group of proteins which bind specifically to the carbohydrates in glycoconjugates. Several fungal lectins target their specificities towards oligosaccharides present in mammalian glycoproteins, thus constituting excellent ligands for the preparation of affinity adsorbents useful in isolation and characterization of these glycoproteins. In this study we isolated and characterized two different proteins, a lectin and a laccase, present in extracts from Punctularia atropurpurascens. The lectin isolated from the mycelium extract, was immobilized on activated-Sepharose and used to evaluate the interaction with three glycoproteins. The adsorbent was able to efficiently adsorb and elute bovine lactoferrin, constituting a promising tool for the purification of this glycoprotein. In vitro experiments revealed that the lectin also exhibited antimicrobial activity against Aspergillus niger. Laccase activity was detected in the extracellular extract from P. atropurpurascens. This enzyme, in both soluble and immobilized forms, was able to degrade Remazol Brilliant Blue R and Acid Blue 25 dyes. The biological activities found in this fungus demonstrate its potential for various biotechnological applications.展开更多
Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system ...Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system of ergosterol skeleton.In addition,five new steroids,5β,6β-epoxy-3β,7α,9α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(2),5β,6β-epoxy-3β,7α-dihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(3),5α,6α-epoxy-3β,9α,15α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-7-one(4),15α-acetoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(5),15β-methoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(6),along with four known ergosterols(7-10),were obtained.All structures were elucidated based on 1D and 2D NMR spectral data.New compounds were evaluated for cytotoxicity against five human cancer cell lines,only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.展开更多
Seven new tremulane-type sesquiterpenes （1 7） have been isolated from cultures of the basidiomycete Cono- cybe siliginea. Their structures and relative configurations were elucidated by extensive spectroscopic data ...Seven new tremulane-type sesquiterpenes （1 7） have been isolated from cultures of the basidiomycete Cono- cybe siliginea. Their structures and relative configurations were elucidated by extensive spectroscopic data （HSQC, HMBC, 1H-tH COSY, ROESY） and MS analysis.展开更多
To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were de...To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were designed and tested. Wet cells (>10 g) of 4 basidiomycete strains (Pleurotus salmoneostramineus H7, P. salmoneostramineus H13, Ganoderma lucidum NBRC31863, Flammulina velutipes NBRC31862) were harvested from PGO medium for 7 days. The stereoselective reduction of α-keto esters using the 4 strains was tested. It was found that each of these strains had a reducing activity toward 6 aliphatic α-keto esters. In the presence of L-alanine as an additive, the reduction of ethyl 2-oxobutanoate and ethyl 2-oxopentanoete by P. salmoneostramineus H7 produced the corresponding alcohol with a high conversion ratio and with excellent enantiomeric excess (>99% e.e. (R)). Furthermore, ethyl pyruvate, ethyl 2-oxobutanoate, and ethyl 2-oxopentanoate were predominantly reduced to the corresponding (R)-hydroxy ester (>99% e.e.) by G. lucidum. Thus, we found that these edible mushrooms have great potential to be used as biocatalysts for the stereoselective reduction of carbonyl compounds.展开更多
Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaust...Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaustraldehydes A-E(3-7),and three tetra-nor ones ganoaustrenoic acids A-C(8-10).The chemical structures along with the absolute configurations were determined by extensive spectroscopic analysis of 1D&2D NMR and HRESIMS data.The postulated biosynthesis pathways of these compounds were proposed.Ganoaustraldehydes A(3)and B(4)showed moderate inhibition against nitric oxide production in RAW264.7 macrophage cells with the respec-tive IC_(50) values of 32.5,34.2μM(the IC_(50) of positive control pyrrolidine dithiocarbamate was 20.0μM).展开更多
基金This project was supported bythe National Natural Science Foundation of China(30225048and30470027)and the Natural Sci-ence Foundation of Yunnan Province(2005C0052Mand2005C0011R)
文摘Basidiomycetes are able to biodegrade waste and xenobiotic molecules through the production of extracellular enzymes. For example, white-rot fungi produce lignin-degrading enzymes which are capable of efficiently decolorizing dye solutions. Many mushrooms also produce lectins, a group of proteins which bind specifically to the carbohydrates in glycoconjugates. Several fungal lectins target their specificities towards oligosaccharides present in mammalian glycoproteins, thus constituting excellent ligands for the preparation of affinity adsorbents useful in isolation and characterization of these glycoproteins. In this study we isolated and characterized two different proteins, a lectin and a laccase, present in extracts from Punctularia atropurpurascens. The lectin isolated from the mycelium extract, was immobilized on activated-Sepharose and used to evaluate the interaction with three glycoproteins. The adsorbent was able to efficiently adsorb and elute bovine lactoferrin, constituting a promising tool for the purification of this glycoprotein. In vitro experiments revealed that the lectin also exhibited antimicrobial activity against Aspergillus niger. Laccase activity was detected in the extracellular extract from P. atropurpurascens. This enzyme, in both soluble and immobilized forms, was able to degrade Remazol Brilliant Blue R and Acid Blue 25 dyes. The biological activities found in this fungus demonstrate its potential for various biotechnological applications.
基金supported by National Basic Research Program of China(973 Program,2009CB522300)the National Natural Science Foundation of China(30830113,U1132607).
文摘Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system of ergosterol skeleton.In addition,five new steroids,5β,6β-epoxy-3β,7α,9α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(2),5β,6β-epoxy-3β,7α-dihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(3),5α,6α-epoxy-3β,9α,15α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-7-one(4),15α-acetoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(5),15β-methoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(6),along with four known ergosterols(7-10),were obtained.All structures were elucidated based on 1D and 2D NMR spectral data.New compounds were evaluated for cytotoxicity against five human cancer cell lines,only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.
文摘Seven new tremulane-type sesquiterpenes （1 7） have been isolated from cultures of the basidiomycete Cono- cybe siliginea. Their structures and relative configurations were elucidated by extensive spectroscopic data （HSQC, HMBC, 1H-tH COSY, ROESY） and MS analysis.
文摘To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were designed and tested. Wet cells (>10 g) of 4 basidiomycete strains (Pleurotus salmoneostramineus H7, P. salmoneostramineus H13, Ganoderma lucidum NBRC31863, Flammulina velutipes NBRC31862) were harvested from PGO medium for 7 days. The stereoselective reduction of α-keto esters using the 4 strains was tested. It was found that each of these strains had a reducing activity toward 6 aliphatic α-keto esters. In the presence of L-alanine as an additive, the reduction of ethyl 2-oxobutanoate and ethyl 2-oxopentanoete by P. salmoneostramineus H7 produced the corresponding alcohol with a high conversion ratio and with excellent enantiomeric excess (>99% e.e. (R)). Furthermore, ethyl pyruvate, ethyl 2-oxobutanoate, and ethyl 2-oxopentanoate were predominantly reduced to the corresponding (R)-hydroxy ester (>99% e.e.) by G. lucidum. Thus, we found that these edible mushrooms have great potential to be used as biocatalysts for the stereoselective reduction of carbonyl compounds.
基金National Natural Science Foundation of China (grant numbers 21961142008,22177138) for fundings.
文摘Chemical investigation on the medicinal fungus Ganoderma australe led to the identification of ten new nor-lanostane triterpenes,namely two hexa-nor ones,ganoaustratetraenones A(1)and B(2),five penta-nor ones,ganoaustraldehydes A-E(3-7),and three tetra-nor ones ganoaustrenoic acids A-C(8-10).The chemical structures along with the absolute configurations were determined by extensive spectroscopic analysis of 1D&2D NMR and HRESIMS data.The postulated biosynthesis pathways of these compounds were proposed.Ganoaustraldehydes A(3)and B(4)showed moderate inhibition against nitric oxide production in RAW264.7 macrophage cells with the respec-tive IC_(50) values of 32.5,34.2μM(the IC_(50) of positive control pyrrolidine dithiocarbamate was 20.0μM).
基金This work was supported by the National Natural Science Foundation of China(30470027and30225048)the Natural Science Foundation of Yunnan Province(2005C0011Rand2005C0052M).
