We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative ...We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus （ABV）. Southern blotting and inverse polymerase chain reaction （PCR） data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geo- graphical distribution as well as the host range of the badnaviruses.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.31370181 and 31570146)the Fujian Natural Science Funds for Distinguished Young Scholar(No.2014J06008),China
文摘We report the recovery of a 7068-nt viral sequence from the ＂viral fossils＂ embedded in the genome of Alhagi sparsifolia, a typical desert plant. Although the full viral genome remains to be completed, the putative genome structure, the deduced amino acids and phylogenetic analysis unambiguously demonstrate that this viral sequence represents a novel species of the genus Badnavirus. The putative virus is tentatively termed Alhagi bacilliform virus （ABV）. Southern blotting and inverse polymerase chain reaction （PCR） data indicate that the ABV-related sequence is integrated into the A. sparsifolia genome, and probably does not give rise to functional episomal virus. Molecular evidence that the ABV sequence exists widely in A. sparsifolia is also presented. To our knowledge, this is the first endogenous badnavirus identified from plants in the Gobi desert, and may provide new clues on the evolution, geo- graphical distribution as well as the host range of the badnaviruses.
