通过对养殖海水以及捕获、加工、流通和冰藏等整个供应链过程养殖大黄鱼的温度履历、感官、化学、微生物的品质监测,评估养殖海水、碎冰和加工过程中大黄鱼细菌卫生状况,分析其在整个过程鲜度和细菌相变化。结果表明,养殖海水和碎冰的...通过对养殖海水以及捕获、加工、流通和冰藏等整个供应链过程养殖大黄鱼的温度履历、感官、化学、微生物的品质监测,评估养殖海水、碎冰和加工过程中大黄鱼细菌卫生状况,分析其在整个过程鲜度和细菌相变化。结果表明,养殖海水和碎冰的菌落总数分别为4.99±0.41 lg cfu/g和3.90±0.32 lg cfu/g,大肠菌群数分别为30~450 MNP/100 g和低于30 MNP/100 g;新捕获养殖大黄鱼的菌落总数为3.82±0.38 lg cfu/g,假单胞菌数为3.13±0.58 lg cfu/g,嗜冷菌数为2.82±0.60 lg cfu/g,大肠菌群数为低于30 MPN/100 g;冰藏第18天时TVBN含量为28.62±0.51 mg/100 g,菌落总数、假单胞菌数、嗜冷菌数和大肠菌群数分别为6.24±0.06lg cfu/g、6.01±0.25 lg cfu/g、5.68±0.21 lg cfu/g和30 MPN/100 g。养殖海水和新捕获鱼中细菌种类繁多,革兰氏阴性菌分别占其总菌株数的64.9%和56.8%;革兰氏阳性菌分别占其总菌株数的27.0%和42.0%;优势菌群为黏性威克斯菌、腐败希瓦氏菌和假单胞菌属。加工结束后,假单胞菌（34.9%）、玫瑰小球菌（32.6%）和缺陷短波单胞菌（14.0%）成为主要菌群。冰藏第4天时仅分离出4种不同类型的细菌,腐败希瓦氏菌比例上升明显,冰藏第10天和第18天时,所占比例分别为63.5%和69.1%,是冷却链养殖大黄鱼产品的优势腐败菌。展开更多
AIM： To investigate the non-He/icobacterpy/ori （H. pylori） bacterial flora concurrent with H. pylori infection.METHODS： A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for ba...AIM： To investigate the non-He/icobacterpy/ori （H. pylori） bacterial flora concurrent with H. pylori infection.METHODS： A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H, pylori bacterial isolates were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF MS）.RESULTS： A total of 201 non-H, pyiori bacterial isolates were cultivated from 67 （65.0%） of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothla and Staphylococcus, which differed fromthe predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group （100% vs 42.9%, P 〈 0.001）. Six bacterial species with urease activity （Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacteriurn spp. and Klebsiella pneumoniae） were also isolated.CONCLUSION： There is a high prevalence of the non-H, pylori bacteria concurrent with H. pylori infection, and the non-H, pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.展开更多
文摘通过对养殖海水以及捕获、加工、流通和冰藏等整个供应链过程养殖大黄鱼的温度履历、感官、化学、微生物的品质监测,评估养殖海水、碎冰和加工过程中大黄鱼细菌卫生状况,分析其在整个过程鲜度和细菌相变化。结果表明,养殖海水和碎冰的菌落总数分别为4.99±0.41 lg cfu/g和3.90±0.32 lg cfu/g,大肠菌群数分别为30~450 MNP/100 g和低于30 MNP/100 g;新捕获养殖大黄鱼的菌落总数为3.82±0.38 lg cfu/g,假单胞菌数为3.13±0.58 lg cfu/g,嗜冷菌数为2.82±0.60 lg cfu/g,大肠菌群数为低于30 MPN/100 g;冰藏第18天时TVBN含量为28.62±0.51 mg/100 g,菌落总数、假单胞菌数、嗜冷菌数和大肠菌群数分别为6.24±0.06lg cfu/g、6.01±0.25 lg cfu/g、5.68±0.21 lg cfu/g和30 MPN/100 g。养殖海水和新捕获鱼中细菌种类繁多,革兰氏阴性菌分别占其总菌株数的64.9%和56.8%;革兰氏阳性菌分别占其总菌株数的27.0%和42.0%;优势菌群为黏性威克斯菌、腐败希瓦氏菌和假单胞菌属。加工结束后,假单胞菌（34.9%）、玫瑰小球菌（32.6%）和缺陷短波单胞菌（14.0%）成为主要菌群。冰藏第4天时仅分离出4种不同类型的细菌,腐败希瓦氏菌比例上升明显,冰藏第10天和第18天时,所占比例分别为63.5%和69.1%,是冷却链养殖大黄鱼产品的优势腐败菌。
基金Supported by The major projects of infectious disease prevention and control in China,including AIDS and viral hepatitis,No.2008ZX10004-002 and No.2009ZX10603
文摘AIM： To investigate the non-He/icobacterpy/ori （H. pylori） bacterial flora concurrent with H. pylori infection.METHODS： A total of 103 gastric biopsy specimens from H. pylori positive patients were selected for bacterial culture. All the non-H, pylori bacterial isolates were identified by matrix assisted laser desorption ionization time-of-flight mass spectrometry （MALDI-TOF MS）.RESULTS： A total of 201 non-H, pyiori bacterial isolates were cultivated from 67 （65.0%） of the 103 gastric samples, including 153 isolates identified successfully at species level and 48 at genus level by MALDI-TOF MS. The dominant species were Streptococcus, Neisseria, Rothla and Staphylococcus, which differed fromthe predominantly acid resistant species reported previously in healthy volunteers. The prevalence of non-H. pylori bacteria was higher in non-ulcer dyspepsia group than in gastric ulcer group （100% vs 42.9%, P 〈 0.001）. Six bacterial species with urease activity （Staphylococcus epidermidis, Staphylococcus warneri, Staphylococcus capitis, Staphylococcus aureus, Brevibacteriurn spp. and Klebsiella pneumoniae） were also isolated.CONCLUSION： There is a high prevalence of the non-H, pylori bacteria concurrent with H. pylori infection, and the non-H, pylori bacteria may also play important as-yet-undiscovered roles in the pathogenesis of stomach disorders.
