为获得稻田土壤镉污染修复的微生物,采用培养基加镉平板法,从水稻根际土壤分离耐镉细菌。本研究分离得到3株耐镉菌株,经16S rDNA测序、比对,鉴定为假单胞菌属细菌。对菌株细胞形态的电镜观察、菌株生长曲线的测定结果表明,在镉处理浓度...为获得稻田土壤镉污染修复的微生物,采用培养基加镉平板法,从水稻根际土壤分离耐镉细菌。本研究分离得到3株耐镉菌株,经16S rDNA测序、比对,鉴定为假单胞菌属细菌。对菌株细胞形态的电镜观察、菌株生长曲线的测定结果表明,在镉处理浓度为100 mg L 1条件下,菌株生长未受明显抑制,但菌株形态发生了适应性的变化;在镉处理浓度超过300 mg L 1时,菌株生长受到明显抑制;在镉处理浓度700 mg L 1条件下,菌株仍能生长,但生长延滞期延长,对数期后的生长量亦低;对菌体镉含量分析表明,在100 mg L 1镉处理下,3株菌株菌体中的镉含量分别为9.04 mg g 1、4.96 mg g 1和28.58 mg g 1,富集系数依次为90.4、49.6和285.8。可见,分离得到的3株菌株具有较强镉耐性和镉吸收能力,将为稻田土壤重金属镉污染的修复提供重要微生物资源。展开更多
Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the p...Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the process of ammonia nitrogen removal ,related data between ammonia removal and five factors was received through multi-factor orthogonal test,and established relations model between the five factor and nitrogen removal. The results show that five-factors had significant effect on AR,and the best combinations for removing AR was temperature 30 ℃,pH=7.0,light intensity 6 000 lux,dissolved oxygen 5.0 mg/L and the fill rate 10%. According to the experimental data,equation model was proposed and coefficient of determination R2 =0.864 8,P<0.05. Samples T-test was done between the model predictions and the actual measured values.Test results showed that the significant difference of overall mean value sig. (2-tailed) was 0.978 (P>0.05),it Shows that had no significant difference between model predictions and the actual measured value,and model had a high degree of fitting.展开更多
文摘为获得稻田土壤镉污染修复的微生物,采用培养基加镉平板法,从水稻根际土壤分离耐镉细菌。本研究分离得到3株耐镉菌株,经16S rDNA测序、比对,鉴定为假单胞菌属细菌。对菌株细胞形态的电镜观察、菌株生长曲线的测定结果表明,在镉处理浓度为100 mg L 1条件下,菌株生长未受明显抑制,但菌株形态发生了适应性的变化;在镉处理浓度超过300 mg L 1时,菌株生长受到明显抑制;在镉处理浓度700 mg L 1条件下,菌株仍能生长,但生长延滞期延长,对数期后的生长量亦低;对菌体镉含量分析表明,在100 mg L 1镉处理下,3株菌株菌体中的镉含量分别为9.04 mg g 1、4.96 mg g 1和28.58 mg g 1,富集系数依次为90.4、49.6和285.8。可见,分离得到的3株菌株具有较强镉耐性和镉吸收能力,将为稻田土壤重金属镉污染的修复提供重要微生物资源。
基金Supported by the National Natural Science Foundation of China(No.30972260)~~
文摘Applied Immobilized algae bacteria (ABI) to remove ammonia of freshwater aquaculture wastewater. Temperature (T),PH,light intensity (I),dissolved oxygen (DO) and filling rate five factors plays important role in the process of ammonia nitrogen removal ,related data between ammonia removal and five factors was received through multi-factor orthogonal test,and established relations model between the five factor and nitrogen removal. The results show that five-factors had significant effect on AR,and the best combinations for removing AR was temperature 30 ℃,pH=7.0,light intensity 6 000 lux,dissolved oxygen 5.0 mg/L and the fill rate 10%. According to the experimental data,equation model was proposed and coefficient of determination R2 =0.864 8,P<0.05. Samples T-test was done between the model predictions and the actual measured values.Test results showed that the significant difference of overall mean value sig. (2-tailed) was 0.978 (P>0.05),it Shows that had no significant difference between model predictions and the actual measured value,and model had a high degree of fitting.
