乳腺癌是女性最常见的恶性肿瘤之一,在全球范围内,发病率呈逐年上升的趋势,在国内多个大中城市中,它已成为威胁女性健康的恶性肿瘤首位死因。BRCA1（breast cancer sus-ceptibility gene 1）是遗传性乳腺癌和卵巢癌的易感基因,Miki等[1]...乳腺癌是女性最常见的恶性肿瘤之一,在全球范围内,发病率呈逐年上升的趋势,在国内多个大中城市中,它已成为威胁女性健康的恶性肿瘤首位死因。BRCA1（breast cancer sus-ceptibility gene 1）是遗传性乳腺癌和卵巢癌的易感基因,Miki等[1]在1994年首次成功地将其定位和分离。经过国内外学者大量的研究发现，BRCAI的基因结构和功能异常与乳腺癌的发生发展有着密切的关系，可作为一种肿瘤抑制因子而发挥作用，是目前发现的与乳腺癌发生相关的关键的抑癌基因。展开更多
The BRCA1 （Breast Cancer Anti-estrogen resistance-I）, early-onset gene is expressed in cells of breast and other tissue and helps to repair damaged DNA or destroy cells in cases DNA cannot be repaired. When the BRCA...The BRCA1 （Breast Cancer Anti-estrogen resistance-I）, early-onset gene is expressed in cells of breast and other tissue and helps to repair damaged DNA or destroy cells in cases DNA cannot be repaired. When the BRCA1 gene is damaged, then the DNA is not repaired appropriately and this enhances the risk for cancer. Fluorescence and UV-visible thermal studies were performed for WT （wild type） and MT （mutant type targets） full systems. The target DNAs used were in the form of short oligonucleotides, genomic DNA. The probe system was used for detection of WT and SNP alleles of human BRCAI [（170-190, G---~T） and （290-310, G---~T）]. The Cy5 dye attached to a probe oligonucleotide （10-mer） undergoes a fluorescence intensity change on hybridisation of the probe to the WT compared to MT targets. Our results indicate that the system consisting of the target sequence and the one probe oligonucleotides bearing the Cy5 dye assemble correctly at the specified target. Once the full system （probe and target） is arranged under suitable conditions, a red-shift emission and change in fluorescence intensity are seen at a suitable wavelength. Thermal studies also showed significant differences in T,, between WT and MT. The results suggest that the differences in the fluorescence intensity at 665 nm and the spectrophotometric T,,,cs） for the WT and MT can be attributed to the type of binding of the probe to the target. The systems were sensitive to single nucleotide polymorphisms and this may help in high throughput applications in genetic testing and molecular diagnostics.展开更多
文摘乳腺癌是女性最常见的恶性肿瘤之一,在全球范围内,发病率呈逐年上升的趋势,在国内多个大中城市中,它已成为威胁女性健康的恶性肿瘤首位死因。BRCA1（breast cancer sus-ceptibility gene 1）是遗传性乳腺癌和卵巢癌的易感基因,Miki等[1]在1994年首次成功地将其定位和分离。经过国内外学者大量的研究发现，BRCAI的基因结构和功能异常与乳腺癌的发生发展有着密切的关系，可作为一种肿瘤抑制因子而发挥作用，是目前发现的与乳腺癌发生相关的关键的抑癌基因。
文摘The BRCA1 （Breast Cancer Anti-estrogen resistance-I）, early-onset gene is expressed in cells of breast and other tissue and helps to repair damaged DNA or destroy cells in cases DNA cannot be repaired. When the BRCA1 gene is damaged, then the DNA is not repaired appropriately and this enhances the risk for cancer. Fluorescence and UV-visible thermal studies were performed for WT （wild type） and MT （mutant type targets） full systems. The target DNAs used were in the form of short oligonucleotides, genomic DNA. The probe system was used for detection of WT and SNP alleles of human BRCAI [（170-190, G---~T） and （290-310, G---~T）]. The Cy5 dye attached to a probe oligonucleotide （10-mer） undergoes a fluorescence intensity change on hybridisation of the probe to the WT compared to MT targets. Our results indicate that the system consisting of the target sequence and the one probe oligonucleotides bearing the Cy5 dye assemble correctly at the specified target. Once the full system （probe and target） is arranged under suitable conditions, a red-shift emission and change in fluorescence intensity are seen at a suitable wavelength. Thermal studies also showed significant differences in T,, between WT and MT. The results suggest that the differences in the fluorescence intensity at 665 nm and the spectrophotometric T,,,cs） for the WT and MT can be attributed to the type of binding of the probe to the target. The systems were sensitive to single nucleotide polymorphisms and this may help in high throughput applications in genetic testing and molecular diagnostics.
