目的观察NGF、BDNF和NT3在A lzhe im er d isease(AD)大鼠海马中的分布及其表达变化。方法采用海马注射Aβ淀粉蛋白的方法建立AD模型。10 d后对大鼠进行灌注,取脑,冰冻切片,用NGF、BNDF和NT3抗体行免疫组织化学染色。对海马恒定视野内NG...目的观察NGF、BDNF和NT3在A lzhe im er d isease(AD)大鼠海马中的分布及其表达变化。方法采用海马注射Aβ淀粉蛋白的方法建立AD模型。10 d后对大鼠进行灌注,取脑,冰冻切片,用NGF、BNDF和NT3抗体行免疫组织化学染色。对海马恒定视野内NGF、BDNF和NT3的阳性细胞进行记数并进行统计学分析。结果AD组海马中的NGF阳性细胞较正常组显著增多(P<0.01),且染色增强。BDNF阳性细胞较正常组明显减少(P<0.01),染色强度减弱。而NT3的阳性细胞数及染色强度与正常组比较差异均没有统计学意义(P>0.05)。结论NGF、BDNF和NT3在AD的海马中发生了不同的变化,提示NGF、BDNF和NT3在AD中发挥了不同的作用,尤其是BDNF阳性细胞的减少可能与AD的神经功能减退有关。展开更多
Various studies have shown that the architecture and design of a nursing home can have a profound impact on the sense of home of old people residing in the nursing home, next to psychological and social factors. Howev...Various studies have shown that the architecture and design of a nursing home can have a profound impact on the sense of home of old people residing in the nursing home, next to psychological and social factors. However, adequate guidance on how these factors can be operationalized in practice is not provided for architects and interior designers. This study investigated which architectural factors contribute to a sense of home and how these can be implemented in the design guidelines. Two existing data sets were used, combining the most recent evidence from the literature and experiences of residents, family caregivers, and professional staff of Dutch nursing homes. These analyses resulted in theoretical implications for the private space, quasi-public space, the took and feel of the nursing home, and the outdoors. Furthermore, these analyses were used for the design of a demonstration apartment that integrates the factors of the sense of home. This description was concluded by a checklist for practice, in which design guidelines were formulated. A holistic understanding of which factors influence the sense of home could tead to improvements of the sense of home of nursing home residents.展开更多
文摘目的观察NGF、BDNF和NT3在A lzhe im er d isease(AD)大鼠海马中的分布及其表达变化。方法采用海马注射Aβ淀粉蛋白的方法建立AD模型。10 d后对大鼠进行灌注,取脑,冰冻切片,用NGF、BNDF和NT3抗体行免疫组织化学染色。对海马恒定视野内NGF、BDNF和NT3的阳性细胞进行记数并进行统计学分析。结果AD组海马中的NGF阳性细胞较正常组显著增多(P<0.01),且染色增强。BDNF阳性细胞较正常组明显减少(P<0.01),染色强度减弱。而NT3的阳性细胞数及染色强度与正常组比较差异均没有统计学意义(P>0.05)。结论NGF、BDNF和NT3在AD的海马中发生了不同的变化,提示NGF、BDNF和NT3在AD中发挥了不同的作用,尤其是BDNF阳性细胞的减少可能与AD的神经功能减退有关。
文摘Various studies have shown that the architecture and design of a nursing home can have a profound impact on the sense of home of old people residing in the nursing home, next to psychological and social factors. However, adequate guidance on how these factors can be operationalized in practice is not provided for architects and interior designers. This study investigated which architectural factors contribute to a sense of home and how these can be implemented in the design guidelines. Two existing data sets were used, combining the most recent evidence from the literature and experiences of residents, family caregivers, and professional staff of Dutch nursing homes. These analyses resulted in theoretical implications for the private space, quasi-public space, the took and feel of the nursing home, and the outdoors. Furthermore, these analyses were used for the design of a demonstration apartment that integrates the factors of the sense of home. This description was concluded by a checklist for practice, in which design guidelines were formulated. A holistic understanding of which factors influence the sense of home could tead to improvements of the sense of home of nursing home residents.
