AIM:To determine the resistance patterns of Helicobacter pylori(H.pylori) strains isolated from patients in Beijing and monitor the change of antibiotic resistance over time.METHODS:In this prospective,serial and cros...AIM:To determine the resistance patterns of Helicobacter pylori(H.pylori) strains isolated from patients in Beijing and monitor the change of antibiotic resistance over time.METHODS:In this prospective,serial and crosssectional study,H.pylori cultures were successfully obtained from 371 and 950 patients(never receiving eradication) during 2009-2010 and 2013-2014,respectively.Resistance to amoxicillin,clarithromycin,metronidazole,levofloxacin,tetracycline,and rifampicin was determined by Epsilometer test.RESULTS:The resistance rates of isolates obtained during 2009-2010 were 66.8%,39.9%,34.5%,15.4%,6.7%,and 4.9% to metronidazole,clarithromycin,levofloxacin,rifampicin,amoxicillin and tetracycline,respectively; and the corresponding rates for isolates during 2013-2014 were 63.4%,52.6%,54.8%,18.2%,4.4% and 7.3%,respectively.The resistance rates to clarithromycin and levofloxacin were significantly increased after four years.In 2009-2010,14.6% of H.pylori isolates were susceptible to all tested antibiotics,with mono(33.7%),double(28.3%),triple(16.7%),quadruple(6.2%),quintuple(0.3%) and sextuple resistance(0.3%) also being detected.In 2013-2014,9.4% were susceptible to all tested antibiotics,and mono(27.6%),double(28.4%),triple(24.9%),quadruple(7.3%),quintuple(2.3%) and sextuple resistance(0.1%) was also observed.More multiple resistant H.pylori isolates were found during 2013-2014.Gender(to levofloxacin and metronidazole),age(to levofloxacin) and endoscopic findings(to clarithromycin) were independent factors influencing antibiotic resistance.CONCLUSION:H.pylori resistance to commonly used antibiotics in Beijing is high with increased multiple antibiotic resistance.展开更多
目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌...目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌株对甲硝唑及克拉霉素的最低抑菌浓度(MIC)。对所有入选患者采用兰索拉唑、甲硝唑和克拉霉素的三联7d 疗法进行 Hp 根除治疗。结果共有157例患者进入本研究,并完成了治疗。Hp 对甲硝唑和克拉霉素的耐药率分别为:45.9％(72/157)和18.5％(29/157)。157例接受治疗患者的 Hp 根除率为61.8％(97/157),Hp 根除率在不同性别、年龄及不同临床疾病患者之间差异均无统计学意义(均 P>0.05);甲硝唑敏感菌株和耐药菌株的根除率分别为89.4％(76/85)和29.2％(21/72)(P<0.01)。克拉霉素敏感菌株和耐药菌株的根除率分别为72.7％(93/128)和16.0％(4/29)(P<0.01)。结论 Hp 对抗生素耐药是导致Hp 根除治疗失败的主要原因。展开更多
AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shangh...AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shanghai and tested for their susceptibility to metronidazole, clarithromycin, amoxicillin, furazolidone, levofloxacin and tetracycline using agar dilution. RESULTS: The resistant rates of H. pylori to clarithromycin (8.6%, 9.0% and 20.7%) and levofloxacin (10.3%, 24.0% and 32.5%) increased from 2000 to 2009 in Shanghai. The resistant rate of H. pylori to metronidazole remained stable (40%-50%). Only one strain of H. pylori isolated in 2005 was resistant to tetracycline. All strains were sensitive to amoxicillin and furazolidone.The resistant rate of H. pylori to antibiotics was not related with the sex, age and clinical outcome of patients. CONCLUSION: Resistance of H. pylori to antibiotics plays an important role in making treatment strategies against H. pylori -associated diseases.展开更多
基金Supported by National Science and Technology Pillar Program during the Twelfth Five-year Plan Period,No.2012BAI06B02the Capital Health Research and Development of Special,No.2011-4032-02
文摘AIM:To determine the resistance patterns of Helicobacter pylori(H.pylori) strains isolated from patients in Beijing and monitor the change of antibiotic resistance over time.METHODS:In this prospective,serial and crosssectional study,H.pylori cultures were successfully obtained from 371 and 950 patients(never receiving eradication) during 2009-2010 and 2013-2014,respectively.Resistance to amoxicillin,clarithromycin,metronidazole,levofloxacin,tetracycline,and rifampicin was determined by Epsilometer test.RESULTS:The resistance rates of isolates obtained during 2009-2010 were 66.8%,39.9%,34.5%,15.4%,6.7%,and 4.9% to metronidazole,clarithromycin,levofloxacin,rifampicin,amoxicillin and tetracycline,respectively; and the corresponding rates for isolates during 2013-2014 were 63.4%,52.6%,54.8%,18.2%,4.4% and 7.3%,respectively.The resistance rates to clarithromycin and levofloxacin were significantly increased after four years.In 2009-2010,14.6% of H.pylori isolates were susceptible to all tested antibiotics,with mono(33.7%),double(28.3%),triple(16.7%),quadruple(6.2%),quintuple(0.3%) and sextuple resistance(0.3%) also being detected.In 2013-2014,9.4% were susceptible to all tested antibiotics,and mono(27.6%),double(28.4%),triple(24.9%),quadruple(7.3%),quintuple(2.3%) and sextuple resistance(0.1%) was also observed.More multiple resistant H.pylori isolates were found during 2013-2014.Gender(to levofloxacin and metronidazole),age(to levofloxacin) and endoscopic findings(to clarithromycin) were independent factors influencing antibiotic resistance.CONCLUSION:H.pylori resistance to commonly used antibiotics in Beijing is high with increased multiple antibiotic resistance.
文摘目的了解幽门螺杆菌(Hp)耐药菌株以及患者性别、年龄和不同临床疾病对 Hp 根除治疗的影响,阐明 Hp 根除治疗失败的主要原因。方法对因上胃肠道症状而来北京大学第一医院胃镜室接受胃镜检查的 Hp 培养阳性患者,采用 E-试验法测定 Hp 菌株对甲硝唑及克拉霉素的最低抑菌浓度(MIC)。对所有入选患者采用兰索拉唑、甲硝唑和克拉霉素的三联7d 疗法进行 Hp 根除治疗。结果共有157例患者进入本研究,并完成了治疗。Hp 对甲硝唑和克拉霉素的耐药率分别为:45.9％(72/157)和18.5％(29/157)。157例接受治疗患者的 Hp 根除率为61.8％(97/157),Hp 根除率在不同性别、年龄及不同临床疾病患者之间差异均无统计学意义(均 P>0.05);甲硝唑敏感菌株和耐药菌株的根除率分别为89.4％(76/85)和29.2％(21/72)(P<0.01)。克拉霉素敏感菌株和耐药菌株的根除率分别为72.7％(93/128)和16.0％(4/29)(P<0.01)。结论 Hp 对抗生素耐药是导致Hp 根除治疗失败的主要原因。
基金Supported by National Natural Science Foundation of China,No. 30770112Shanghai Leading Academic Discipline Project, No. Y0205
文摘AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shanghai and tested for their susceptibility to metronidazole, clarithromycin, amoxicillin, furazolidone, levofloxacin and tetracycline using agar dilution. RESULTS: The resistant rates of H. pylori to clarithromycin (8.6%, 9.0% and 20.7%) and levofloxacin (10.3%, 24.0% and 32.5%) increased from 2000 to 2009 in Shanghai. The resistant rate of H. pylori to metronidazole remained stable (40%-50%). Only one strain of H. pylori isolated in 2005 was resistant to tetracycline. All strains were sensitive to amoxicillin and furazolidone.The resistant rate of H. pylori to antibiotics was not related with the sex, age and clinical outcome of patients. CONCLUSION: Resistance of H. pylori to antibiotics plays an important role in making treatment strategies against H. pylori -associated diseases.
