从人工培样的灰树花子实体中,经过DEAE Sepharose Fast Flow阴离子交换树脂和ToyopearlHW-75分子筛分离纯化得到一种多糖。运用紫外扫描,薄层层析,高效液相色谱法进行纯度鉴定和红外光谱、核磁共振进行结构鉴定。结果表明,在经过分离纯...从人工培样的灰树花子实体中,经过DEAE Sepharose Fast Flow阴离子交换树脂和ToyopearlHW-75分子筛分离纯化得到一种多糖。运用紫外扫描,薄层层析,高效液相色谱法进行纯度鉴定和红外光谱、核磁共振进行结构鉴定。结果表明,在经过分离纯化后得到了一种α葡聚糖。经过初步药效学实验证明这种多糖可以有效降低KKay小鼠体内血糖含量,并且能够较长期的控制小鼠血糖水平。展开更多
Nigella sativa(N.sativa)(Family Ranunculaceae)is a widely used medicinal plant throughout the world.It is very popular in various traditional systems of medicine like Unani and Tibb,Ayurveda and Siddha.Seeds and oil h...Nigella sativa(N.sativa)(Family Ranunculaceae)is a widely used medicinal plant throughout the world.It is very popular in various traditional systems of medicine like Unani and Tibb,Ayurveda and Siddha.Seeds and oil have a long history of folklore usage in various systems of medicines and food.The seeds of N.saliva have been widely used in the treatment of different diseases and ailments.In Islamic literature,it is considered as one of the greatest forms of healing medicine.It has been recommended for using on regular basis in Tibb-e-Nabwi(Prophetic Medicine).It has been widely used as antihypertensive,liver tonics,diuretics,digestive,anti-diarrheal,appetite stimulant,analgesics,anti-bacterial and in skin disorders.Extensive studies on N.sativa have been carried out by various researchers and a wide spectrum of its pharmacological actions have been explored which may include antidiabetic,anticancer,immunomodulator,analgesic,antimicrobial,anti-inflammatory,spasmolytic,bronchodilator,hepato-protective,renal protective,gaslro-prolective,antioxidant properties,etc.Due to its miraculous power of healing,N.sativa has got the place among the top ranked evidence based herbal medicines.This is also revealed that most of the therapeutic,properties of this plant are due to the presence of thymoquinone which is major bioactive component of the essential oil.The present review is an effort to provide a detailed survey of the literature on scientific researches of pharmacognostical characteristics,chemical composition and pharmacological activities of the seeds of this plant.展开更多
Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced d...Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced diabetic rats.Methods:In this study,the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of all three plants was evaluated by using normal and STZ induced diabetic rots at a dose of 200 mg/kg and 400 mg/kg p.o.daily for 21 days.Blood glucose levels and body weight were monitored at specific intervals,and different biochemical parameters,serum cholesterol,serum triglyceride,high density lipoprotein, low density lipoprotein and very low density lipoprotein were also assessed in the experimental animals.Histology of pancreas was performed.Results:It was found that all the three plants at 400 mg/kg dose showed significant anti-diabetic and anti-cholesterolemic activity(P<0.01),while at 200 mg/kg dose less significant anti-diabetic activity(P<0.05) was observed.Concluslons: Methanol extracts of Amaranthus caudatus,Amaranlhus spinosus and Amaranthus viridis showed significant anti-diabetic and anti-cholesterolemic activity,which provides the scientific proof for their traditional claims.展开更多
建立了特殊医学用途配方食品中非法添加的13种降糖类化学成分的通过式固相萃取净化/超高效液相色谱-串联质谱检测方法。样品用90%乙腈水溶液(含1%甲酸)提取后,提取液经Oasis PRi ME HLB固相萃取柱净化,以Waters ACQUITY UPLC BEH C18(15...建立了特殊医学用途配方食品中非法添加的13种降糖类化学成分的通过式固相萃取净化/超高效液相色谱-串联质谱检测方法。样品用90%乙腈水溶液(含1%甲酸)提取后,提取液经Oasis PRi ME HLB固相萃取柱净化,以Waters ACQUITY UPLC BEH C18(150 mm×2.1 mm,1.7μm)色谱柱分离,以甲醇-含0.1%甲酸的5 mmol/L乙酸铵溶液为流动相梯度洗脱,正离子模式电喷雾电离,配合多反应离子扫描(MRM)定性定量分析目标化合物。考察了提取剂用量、甲酸及乙腈含量对加标回收率的影响,优化了主要的影响因素和实验条件。结果表明:13种化合物在2~100μg/L范围内线性关系良好,相关系数r2≥0.995 1;方法回收率为80.9%~102.8%;定量下限为0.3~1.0μg/kg。该方法前处理简单,回收率高,重现性好,可作为降糖类特殊医学用途配方食品中非法添加降糖类化学成分的有效检测方法。展开更多
Objective:To evaluate the hypoglycemic effect of Brassica juncea(seeds) on streptozotocin induced diabetic male albino rats.Methods:Hypoglycemic activity of Brassica juncea(seeds)aqueous extract at a dose of 250,350 a...Objective:To evaluate the hypoglycemic effect of Brassica juncea(seeds) on streptozotocin induced diabetic male albino rats.Methods:Hypoglycemic activity of Brassica juncea(seeds)aqueous extract at a dose of 250,350 and 450 mg/kg body weight was evaluated.Adult male Swiss albino rats of six numbers in each group was undertaken for study and evaluated.Results:The serum insulin levels were recorded a significant depletion in all groups,short term as well as long term diabetic animals,when compared to that of normal animals.A significant dosage dependent augmenting effect of the seed extract on the serum insulin was recorded in both short term as well as long term groups.Conclusions:The aqueous seed extract of Brassica juncea has potent hypoglycemic activity in male albino rat.展开更多
Exploration of natural sources for novel bioactive compounds has been an emerging field of medicine over the past decades,providing drugs or lead compounds of considerable therapeutic potential.This research has provi...Exploration of natural sources for novel bioactive compounds has been an emerging field of medicine over the past decades,providing drugs or lead compounds of considerable therapeutic potential.This research has provided exciting evidence on the isolation of microbe-derived metabolites having prospective biological activities.Mushrooms have been valued as traditional sources of natural bioactive compounds for many centuries and have been targeted as promising therapeutic agents.Many novel biologically active compounds have been reported as a result of research on medicinal mushrooms.In this review,we compile the information on bioactive structure-elucidated metabolites from macrofungi discovered over the last decade and highlight their unique chemical diversity and potential benefits to novel drug discovery.The main emphasis is on their anti-Alzheimer,antidiabetic,anti-malarial,anti-microbial,anti-oxidant,antitumor,anti-viral and hypocholesterolemic activities which are important medicinal targets in terms of drug discovery today.Moreover,the reader’s attention is brought to focus on mushroom products and food supplements available in the market with claimed biological activities and potential human health benefits.展开更多
Diabetes mellitus is a life-threatening chronic metabolic disease caused by lack of insulin and/or insulin dysfunction,characterized by high levels of glucose in the blood(hyperglycemia).Millions worldwide suffer from...Diabetes mellitus is a life-threatening chronic metabolic disease caused by lack of insulin and/or insulin dysfunction,characterized by high levels of glucose in the blood(hyperglycemia).Millions worldwide suffer from diabetes and its complications.Significantly,it has been recognized that type 2 diabetes is an important preventable disease and can be avoided or delayed by lifestyle intervention.Presently,there are many chemical and biochemical hypoglycemic agents(synthetic drugs),that are used in treating diabetes and are effective in controlling hyperglycemia.However,as they may have harmful side-effects and fail to significantly alter the course of diabetic complications,natural anti-diabetic drugs from medicinal plants have attracted a great deal of attention.Medicinal mushrooms have been valued as a traditional source of natural bioactive compounds over many centuries and have been targeted as potential hypoglycemic and anti-diabetic agents.Bioactive metabolites including polysaccharides,proteins,dietary fibres,and many other biomolecules isolated from medicinal mushrooms and their cultured mycelia have been shown to be successful in diabetes treatment as biological antihyperglycemic agents.In this review we discuss the biological nature of diabetes and,in particular,explore some promising mushrooms that have experimental anti-diabetic properties,preventing or reducing the development of diabetes mellitus.The importance of medicinal mushrooms as agents of medical nutrition therapy and how their metabolites can be used as supportive candidates for prevention and control of diabetes is explored.Future prospects for this field of study and the difficulties and constraints that might affect the development of rational drug products from medicinal mushrooms are discussed.展开更多
文摘从人工培样的灰树花子实体中,经过DEAE Sepharose Fast Flow阴离子交换树脂和ToyopearlHW-75分子筛分离纯化得到一种多糖。运用紫外扫描,薄层层析,高效液相色谱法进行纯度鉴定和红外光谱、核磁共振进行结构鉴定。结果表明,在经过分离纯化后得到了一种α葡聚糖。经过初步药效学实验证明这种多糖可以有效降低KKay小鼠体内血糖含量,并且能够较长期的控制小鼠血糖水平。
基金Supported by Deanship of Scientific Research,King Abdulaziz University,Jeddah,Saudi Arabia(Grant No.431-044)
文摘Nigella sativa(N.sativa)(Family Ranunculaceae)is a widely used medicinal plant throughout the world.It is very popular in various traditional systems of medicine like Unani and Tibb,Ayurveda and Siddha.Seeds and oil have a long history of folklore usage in various systems of medicines and food.The seeds of N.saliva have been widely used in the treatment of different diseases and ailments.In Islamic literature,it is considered as one of the greatest forms of healing medicine.It has been recommended for using on regular basis in Tibb-e-Nabwi(Prophetic Medicine).It has been widely used as antihypertensive,liver tonics,diuretics,digestive,anti-diarrheal,appetite stimulant,analgesics,anti-bacterial and in skin disorders.Extensive studies on N.sativa have been carried out by various researchers and a wide spectrum of its pharmacological actions have been explored which may include antidiabetic,anticancer,immunomodulator,analgesic,antimicrobial,anti-inflammatory,spasmolytic,bronchodilator,hepato-protective,renal protective,gaslro-prolective,antioxidant properties,etc.Due to its miraculous power of healing,N.sativa has got the place among the top ranked evidence based herbal medicines.This is also revealed that most of the therapeutic,properties of this plant are due to the presence of thymoquinone which is major bioactive component of the essential oil.The present review is an effort to provide a detailed survey of the literature on scientific researches of pharmacognostical characteristics,chemical composition and pharmacological activities of the seeds of this plant.
文摘Objective:To investigate the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of Amaranthus caudatus,Amaranthus spinosus and Amaranthus viridis in normal and streptozotocin(STZ) induced diabetic rats.Methods:In this study,the anti-diabetic and anti-cholesterolemic activity of methanol extracts of leaves of all three plants was evaluated by using normal and STZ induced diabetic rots at a dose of 200 mg/kg and 400 mg/kg p.o.daily for 21 days.Blood glucose levels and body weight were monitored at specific intervals,and different biochemical parameters,serum cholesterol,serum triglyceride,high density lipoprotein, low density lipoprotein and very low density lipoprotein were also assessed in the experimental animals.Histology of pancreas was performed.Results:It was found that all the three plants at 400 mg/kg dose showed significant anti-diabetic and anti-cholesterolemic activity(P<0.01),while at 200 mg/kg dose less significant anti-diabetic activity(P<0.05) was observed.Concluslons: Methanol extracts of Amaranthus caudatus,Amaranlhus spinosus and Amaranthus viridis showed significant anti-diabetic and anti-cholesterolemic activity,which provides the scientific proof for their traditional claims.
文摘Objective:To evaluate the hypoglycemic effect of Brassica juncea(seeds) on streptozotocin induced diabetic male albino rats.Methods:Hypoglycemic activity of Brassica juncea(seeds)aqueous extract at a dose of 250,350 and 450 mg/kg body weight was evaluated.Adult male Swiss albino rats of six numbers in each group was undertaken for study and evaluated.Results:The serum insulin levels were recorded a significant depletion in all groups,short term as well as long term diabetic animals,when compared to that of normal animals.A significant dosage dependent augmenting effect of the seed extract on the serum insulin was recorded in both short term as well as long term groups.Conclusions:The aqueous seed extract of Brassica juncea has potent hypoglycemic activity in male albino rat.
基金This study was supported by the grant of the 1551 French-Thai cooperation PHC SIAM 2011(project 25587RA)and the Mae Fah Luang University funded grant“Taxonomy,Phylogeny and cultivation of Lentinus species in northern Thailand”(MFU/54101020048)this study was financially supported by the project“Value added products from basidiomycetes:Putting Thailand’s biodiversity to use”(BRN049/2553)by the National Research Council of Thailand(NRCT)to study medicinal fungi+1 种基金the National Research Council of Thailand(NRCT),project-Taxonomy,Phylogeny and cultivation of Lentinus species in northern Thailand(NRCT/55201020007)the Thailand Research Fund grant-Taxonomy,Phylogeny and biochemistry of Thai Basidiomycetes(BRG 5580009).The authors would also like to acknowledge University Malaya research grants:CG037-2013 Phylogeny,taxonomy,relationships and biotechnological potential of sooty moulds and RG203-12SUS Isolation and structural elucidation of antimicrobial compounds for saprophytic and endophytic fungi of Peninsular Malaysia through bioassay guided fractionation of secondary metabolites extract.
文摘Exploration of natural sources for novel bioactive compounds has been an emerging field of medicine over the past decades,providing drugs or lead compounds of considerable therapeutic potential.This research has provided exciting evidence on the isolation of microbe-derived metabolites having prospective biological activities.Mushrooms have been valued as traditional sources of natural bioactive compounds for many centuries and have been targeted as promising therapeutic agents.Many novel biologically active compounds have been reported as a result of research on medicinal mushrooms.In this review,we compile the information on bioactive structure-elucidated metabolites from macrofungi discovered over the last decade and highlight their unique chemical diversity and potential benefits to novel drug discovery.The main emphasis is on their anti-Alzheimer,antidiabetic,anti-malarial,anti-microbial,anti-oxidant,antitumor,anti-viral and hypocholesterolemic activities which are important medicinal targets in terms of drug discovery today.Moreover,the reader’s attention is brought to focus on mushroom products and food supplements available in the market with claimed biological activities and potential human health benefits.
基金supported by a grant of the 1551 French-Thai cooperation PHC SIAM 2011(project 25587RA)the grants“Taxonomy,Phylogeny and cultivation of Lentinus species in northern Thailand”(MFU/54101020048)+1 种基金from Mae Fah Luang University research division and financially supported by the project“Value added products from basidiomycetes:Putting Thailand’s biodiversity to use”(BRN049/2553)the National Research Council of Thailand(NRCT)to study medicinal fungi.
文摘Diabetes mellitus is a life-threatening chronic metabolic disease caused by lack of insulin and/or insulin dysfunction,characterized by high levels of glucose in the blood(hyperglycemia).Millions worldwide suffer from diabetes and its complications.Significantly,it has been recognized that type 2 diabetes is an important preventable disease and can be avoided or delayed by lifestyle intervention.Presently,there are many chemical and biochemical hypoglycemic agents(synthetic drugs),that are used in treating diabetes and are effective in controlling hyperglycemia.However,as they may have harmful side-effects and fail to significantly alter the course of diabetic complications,natural anti-diabetic drugs from medicinal plants have attracted a great deal of attention.Medicinal mushrooms have been valued as a traditional source of natural bioactive compounds over many centuries and have been targeted as potential hypoglycemic and anti-diabetic agents.Bioactive metabolites including polysaccharides,proteins,dietary fibres,and many other biomolecules isolated from medicinal mushrooms and their cultured mycelia have been shown to be successful in diabetes treatment as biological antihyperglycemic agents.In this review we discuss the biological nature of diabetes and,in particular,explore some promising mushrooms that have experimental anti-diabetic properties,preventing or reducing the development of diabetes mellitus.The importance of medicinal mushrooms as agents of medical nutrition therapy and how their metabolites can be used as supportive candidates for prevention and control of diabetes is explored.Future prospects for this field of study and the difficulties and constraints that might affect the development of rational drug products from medicinal mushrooms are discussed.
