To study IgG subclasses for the hepatitis B virus （HBV） core antigen （anti-HBc） in different populations, a comparison was made between 104 chronic carriers （60 male and 44 female） and 434 recovered individuals ...To study IgG subclasses for the hepatitis B virus （HBV） core antigen （anti-HBc） in different populations, a comparison was made between 104 chronic carriers （60 male and 44 female） and 434 recovered individuals （247 male and 192 female）. Biochemistry analyses of AST （aspartate aminotransferase） and ALT （alanine aminotransferase） were also performed. Among the 104 chronic carriers, 21 patients were found to be ALT and AST abnormal （〉 25 IU/ml）. After comparing these ALT and AST abnormal patients with other ALT and AST normal chronic carriers, no statistical difference was observed in the OD values of the anti-HBe （p 〉 0.05）. The ELISA results showed the anti-HBc IgG subclass pattern was IgG1 〉 IgG3 〉 IgG4 in chronic carriers and IgG3 〉 IgG1 〉 IgG4 in recovered individuals （p 〈 0.05）. This result suggests the IgG1/IgG3 ratio may be related with HBV status. However, in spite of the different anti-HBc IgG1/IgG3 patterns demonstrated in different populations, both anti-HBc IgG1 and IgG3 concentrations were significantly higher in chronic carriers （p 〈 0.05）. Therefore, both the anti-HBc IgG1/IgG3 ratio and their amounts differed. They may play a significant role in chronic carriers and recovered individuals. The anti-HBc IgG subclass profiles of chronic carriers were not changed regardless of liver inflammation, and were independent of sex and age.展开更多
Study background:Anti-HBe and hepatitis B virus(HBV)DNA are important markers in evaluating HBV infection.Anti-HBe indicates past or current infection that persists after hepatitis B e antigen(HBeAg)clearance.Detectin...Study background:Anti-HBe and hepatitis B virus(HBV)DNA are important markers in evaluating HBV infection.Anti-HBe indicates past or current infection that persists after hepatitis B e antigen(HBeAg)clearance.Detecting anti-HBe helps identify cleared or chronic infections.HBV DNA measures viral replication,indicating infection activity.Studying these markers in HBeAg-seronegative young adults provides insights into infection dynamics,immune responses,and transmission risk.It informs clinical management and public health interventions for preventing HBV transmission and reducing the burden of liver disease.Aim and objectives:This cross-sectional study aimed to determine the prevalence of HBeAg seronegativity and its association with HBV infection markers among young adults in Etsako,Edo State,Nigeria.Materials and methods:The study population consisted of HBeAg-seronegative individuals aged 18 to 30 years.A total of 400 participants(200 females and 200 males)were randomly selected from urban and rural communities using a multistage sampling technique.Anti-HBe and HBeAg were analyzed by ELISA,whereas HBV DNA was evaluated by polymerase chain reaction.Results:The prevalence of HBeAg seropositivity was 28%,whereas 72%of participants were seronegative.Among the HBeAg-seronegative participants,the frequencies of anti-HBe and HBV DNA were analyzed based on various categorical variables.The results showed no significant differences in the frequency of anti-HBe and HBV DNA based on occupation,training,or other categorical variables(P>0.05).However,there was a significantly higher proportion of males(60%)among the total number of anti-HBe-seropositive young adults compared to females(40%)(P<0.05).Additionally,a significantly higher proportion of single/unmarried individuals without children(63.3%)was observed among anti-HBe-seropositive young adults compared to those who were married(25%)and those who were single/unmarried with children(12.5%,P<0.05).Conclusion:The study findings contribute to the understanding of HBeAg se展开更多
AIM: To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in sout...AIM: To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in southwest Iran. METHODS: A total of 89 HBsAg-positive serum samples were collected from the same number of patients. All sera were then investigated to determine HBV DNA and serological markers. For all the polymerase chain reaction (PCR)-positive samples, biochemical, histopathological assays and genotyping were also performed. RESULTS: Genotype D was the only type of HBV foundin different clinical forms of acute and chronic infections. There was a high prevalence of HBeAg-negative HBV- infected patients with chronic hepatitis (52.7%). Out of 55 patients with chronic hepatitis, seven (12.7%) were diagnosed with cirrhosis. A significant association between the presence of anti-HBe antibody and an increase in ALT level, among either HBeAg-negative (P = 0.01) or HBeAg-positive (P = 0.026) patients, was demonstrated. No significant differences were observed between the clinical outcomes of HBeAg-positive and -negative individuals (P = 0.24). CONCLUSION: Genotype D has been recognized as the only type of HBV found in different clinical forms of HBV infections, including cirrhosis, among the residents of southwest Iran. Anti-HBe possibly plays a role in disease progression in some patients with chronic hepatitis, at least for a period of disease.展开更多
文摘To study IgG subclasses for the hepatitis B virus （HBV） core antigen （anti-HBc） in different populations, a comparison was made between 104 chronic carriers （60 male and 44 female） and 434 recovered individuals （247 male and 192 female）. Biochemistry analyses of AST （aspartate aminotransferase） and ALT （alanine aminotransferase） were also performed. Among the 104 chronic carriers, 21 patients were found to be ALT and AST abnormal （〉 25 IU/ml）. After comparing these ALT and AST abnormal patients with other ALT and AST normal chronic carriers, no statistical difference was observed in the OD values of the anti-HBe （p 〉 0.05）. The ELISA results showed the anti-HBc IgG subclass pattern was IgG1 〉 IgG3 〉 IgG4 in chronic carriers and IgG3 〉 IgG1 〉 IgG4 in recovered individuals （p 〈 0.05）. This result suggests the IgG1/IgG3 ratio may be related with HBV status. However, in spite of the different anti-HBc IgG1/IgG3 patterns demonstrated in different populations, both anti-HBc IgG1 and IgG3 concentrations were significantly higher in chronic carriers （p 〈 0.05）. Therefore, both the anti-HBc IgG1/IgG3 ratio and their amounts differed. They may play a significant role in chronic carriers and recovered individuals. The anti-HBc IgG subclass profiles of chronic carriers were not changed regardless of liver inflammation, and were independent of sex and age.
文摘Study background:Anti-HBe and hepatitis B virus(HBV)DNA are important markers in evaluating HBV infection.Anti-HBe indicates past or current infection that persists after hepatitis B e antigen(HBeAg)clearance.Detecting anti-HBe helps identify cleared or chronic infections.HBV DNA measures viral replication,indicating infection activity.Studying these markers in HBeAg-seronegative young adults provides insights into infection dynamics,immune responses,and transmission risk.It informs clinical management and public health interventions for preventing HBV transmission and reducing the burden of liver disease.Aim and objectives:This cross-sectional study aimed to determine the prevalence of HBeAg seronegativity and its association with HBV infection markers among young adults in Etsako,Edo State,Nigeria.Materials and methods:The study population consisted of HBeAg-seronegative individuals aged 18 to 30 years.A total of 400 participants(200 females and 200 males)were randomly selected from urban and rural communities using a multistage sampling technique.Anti-HBe and HBeAg were analyzed by ELISA,whereas HBV DNA was evaluated by polymerase chain reaction.Results:The prevalence of HBeAg seropositivity was 28%,whereas 72%of participants were seronegative.Among the HBeAg-seronegative participants,the frequencies of anti-HBe and HBV DNA were analyzed based on various categorical variables.The results showed no significant differences in the frequency of anti-HBe and HBV DNA based on occupation,training,or other categorical variables(P>0.05).However,there was a significantly higher proportion of males(60%)among the total number of anti-HBe-seropositive young adults compared to females(40%)(P<0.05).Additionally,a significantly higher proportion of single/unmarried individuals without children(63.3%)was observed among anti-HBe-seropositive young adults compared to those who were married(25%)and those who were single/unmarried with children(12.5%,P<0.05).Conclusion:The study findings contribute to the understanding of HBeAg se
文摘AIM: To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in southwest Iran. METHODS: A total of 89 HBsAg-positive serum samples were collected from the same number of patients. All sera were then investigated to determine HBV DNA and serological markers. For all the polymerase chain reaction (PCR)-positive samples, biochemical, histopathological assays and genotyping were also performed. RESULTS: Genotype D was the only type of HBV foundin different clinical forms of acute and chronic infections. There was a high prevalence of HBeAg-negative HBV- infected patients with chronic hepatitis (52.7%). Out of 55 patients with chronic hepatitis, seven (12.7%) were diagnosed with cirrhosis. A significant association between the presence of anti-HBe antibody and an increase in ALT level, among either HBeAg-negative (P = 0.01) or HBeAg-positive (P = 0.026) patients, was demonstrated. No significant differences were observed between the clinical outcomes of HBeAg-positive and -negative individuals (P = 0.24). CONCLUSION: Genotype D has been recognized as the only type of HBV found in different clinical forms of HBV infections, including cirrhosis, among the residents of southwest Iran. Anti-HBe possibly plays a role in disease progression in some patients with chronic hepatitis, at least for a period of disease.
