BACKGROUND： Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE： To detect the effect...BACKGROUND： Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE： To detect the effect of different dosages of leptin on luteinizing hormone （LH） and follicle stimulating hormone （FSH） secretion from in vitro cultured rat anterior pituitary cells. DESIGN： Contrast study based on cells. SETTING： This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS： Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS： Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10^-12, 1×10^-11, 1×10^-9, 1×10^-7, and 1×10^-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES： Contents of LH and FSH were detected by radioimmunology. RESULTS： Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10^-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations （P 〈 0.01）. LH release in the leptin （1×10^-12, 1×10^-11, 1×10^-7, and 1×10^-6 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. FSH content in the leptin （1×10^-11, 1×10^-9, and 1×10^-7 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. CONCLUSION： Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a d展开更多
文摘BACKGROUND： Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE： To detect the effect of different dosages of leptin on luteinizing hormone （LH） and follicle stimulating hormone （FSH） secretion from in vitro cultured rat anterior pituitary cells. DESIGN： Contrast study based on cells. SETTING： This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS： Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS： Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10^-12, 1×10^-11, 1×10^-9, 1×10^-7, and 1×10^-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES： Contents of LH and FSH were detected by radioimmunology. RESULTS： Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10^-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations （P 〈 0.01）. LH release in the leptin （1×10^-12, 1×10^-11, 1×10^-7, and 1×10^-6 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. FSH content in the leptin （1×10^-11, 1×10^-9, and 1×10^-7 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. CONCLUSION： Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a d
文摘目的:探讨重症创伤性颅脑损伤(traumatic brain injury,TBI)后垂体前叶激素水平异常影响因素及其对预后的预测价值。方法:回顾性分析2019年3月-2021年3月在厦门市中医院就诊的74例重症TBI患者的临床资料,比较激素分泌异常组及正常组的一般资料,包含年龄、性别、硬膜外血肿情况、硬膜下血肿情况、是否颅底骨折、Glasgow昏迷评分(Glasgow coma scale,GCS评分)、脑疝情况、外伤性蛛网膜下腔出血情况,采用单因素和多因素Logistic回归研究影响垂体前叶激素水平异常的因素。随访3个月后,通过Glasgow预后评分(GOS)分为预后良好(预后良好组,GOS>3分)和预后不良(预后不良组,GOS≤3分),并采用ROC曲线分析垂体前叶激素水平预测患者预后的价值。结果:单因素结果显示出颅底骨折、脑疝、GCS评分均为垂体前叶激素分泌异常的影响因素(P<0.05);Logistic回归结果显示颅底骨折、GCS评分均为垂体前叶激素水平分泌异常的影响因素(P<0.05)。预后良好组TSH为(3.70±0.18)μIU/ml,显著低于预后不良组的(4.51±0.65)μIU/ml;FSH为(18.13±1.20)mIU/L,显著低于预后不良组的(20.59±3.06)mIU/L;LH为(9.75±0.57)mIU/L,显著低于预后不良组的(11.10±0.95)mIU/L;PRL为(479.03±51.45)mIU/L,低于预后不良组的(519.50±61.12)mIU/L,差异有统计学意义(t=8.373、5.000、7.515、2.890,P<0.05)。ROC曲线分析表明,TSH、FSH、LH、PRL预测预后的AUC依次为0.929、0.779、0.911、0.723,差异有统计学意义(P<0.05)。结论:颅底骨折、GCS评分为影响重症TBI患者垂体前叶激素水平分泌异常的危险因素,TSH、FSH、LH、PRL在重症TBI患者预后中具有良好的预测价值,在临床诊断时,医护人员可根据患者实际病例各项指标状况做出合理判断。
