Accumulating evidence indicates that long non-coding RNAs(lncRNAs)can play a pivotal role in regulation of diverse cellular processes.In particular,lncRNAs can serve as master gene regulators at transcriptional and po...Accumulating evidence indicates that long non-coding RNAs(lncRNAs)can play a pivotal role in regulation of diverse cellular processes.In particular,lncRNAs can serve as master gene regulators at transcriptional and posttranscriptional levels,leading to tumorigenesis.In this review,we discuss latest developments in lncRNA-meditated gene expression at the post-transcriptional level,including gene splicing,mRNA stability,protein stability and nuclear trafficking.展开更多
In plants,microRNA (miRNA) functions in the post-transcriptional repression of target mRNAs have been well explored.However,the mechanisms regulating the accumulation of miRNAs remain poorly under.stood.Here,we report...In plants,microRNA (miRNA) functions in the post-transcriptional repression of target mRNAs have been well explored.However,the mechanisms regulating the accumulation of miRNAs remain poorly under.stood.Here,we report that distinct mechanisms regulate accumulation of a monocot-specific miRNA,rice (Oryza sativa) miR528.At the transcriptional level,miR528 accumulated to higher levels in older plants than in young seedlings and exhibited aging-modulated gradual accumulation and diurnal rhythms in leaves;at the post-transcriptional level,aging also modulated miR528 levels by enhancing pri-miR528 alter.native splicing.We found that miR528 promotes rice flowering under long-day conditions by targeting RED AND FAR-RED INSENSITIVE2 (OsRFI2).Moreover,natural variations in the MIR528 promoter region caused differences in miR528 expression among rice varieties,which are correlated with their different binding affinities with the transcription factor OsSPL9 that activates the expression of miR528.Taken together,our findings reveal rice plants have evolved sophisticated modes fine-tuning miR528 levels and provide insight into the mechanisms that regulate MIRNA expression in plants.展开更多
Protein arginine methyltransferases(PRMTs) catalyze the methylation of a variety of protein substrates, many of which have been linked to the development, progression and aggressiveness of different types of cancer. M...Protein arginine methyltransferases(PRMTs) catalyze the methylation of a variety of protein substrates, many of which have been linked to the development, progression and aggressiveness of different types of cancer. Moreover, aberrant expression of PRMTs has been observed in several cancer types. While the link between PRMTs and cancer is a relatively new area of interest, the functional implications documented thus far warrant further investigations into its therapeutic potential. However, the expression of these enzymes and the regulation of their activity in cancer are still significantly understudied. Currently there are nine main members of the PRMT family. Further, the existence of alternatively spliced isoforms for several of these family members provides an additional layer of complexity. Specifically, PRMT1, PRMT2, CARM1 and PRMT7 have been shown to have alternative isoforms and others may be currently unrealized. Our knowledge with respect to the relative expression and the specific functions of these isoforms is largely lacking and needs attention. Here we present a review of the current knowledge of theknown alternative PRMT isoforms and provide a rationale for how they may impact on cancer and represent potentially useful targets for the development of novel therapeutic strategies.展开更多
从福建主栽品种屏优一号PY分离到2个不能正常出菇的单孢菌株PYd21、PYd15,二者配对杂交得到可正常出菇异核菌株H15-21。用solexa测序技术对PYd21基因组从头测序(de novo sequencing),对PYd15、PYd21、H15-21的菌丝体分别进行数字基因表...从福建主栽品种屏优一号PY分离到2个不能正常出菇的单孢菌株PYd21、PYd15,二者配对杂交得到可正常出菇异核菌株H15-21。用solexa测序技术对PYd21基因组从头测序(de novo sequencing),对PYd15、PYd21、H15-21的菌丝体分别进行数字基因表达谱测序,对8个样品(PYd15、PYd21、H15-21的菌丝体,H15-21出菇后的原基、钮扣期菌柄、蛋形期菌柄、伸长期菌柄和成熟期菌柄)mRNA等量混合物进行转录组测序。克隆测序了PYd21与PYd15中磷酸果糖激酶(PFK)基因,比对结果表明二者序列一致。利用转录组数据对PFK基因结构进行分析,结果表明:该基因全长3,494bp,有12个外显子、11个内含子;开放阅读框(ORF)全长2,457bp,编码818个氨基酸,5′端非翻译区(5′UTR)长度281bp,3′端非翻译区(3′UTR)全长103bp;RNA在加工过程中存在1种可变剪切类型、6个可变剪切位点。数字基因表达谱分析结果表明,PFK基因在PYd21、PYd15及H15-21中的标准表达量分别为71.08、120.61、251.85(transcriptper million cleantags,TPM),表达量依次升高,与菌丝生长速度显著正相关。并且异核菌株H15-21表达量高于2个单孢菌株之和,基因表达存在协同增效作用。采用实时荧光定量PCR技术对基因表达量进行验证,表达谱数据切实可靠。展开更多
基金This work was supported by NIH grant R01 CA154989(YM).
文摘Accumulating evidence indicates that long non-coding RNAs(lncRNAs)can play a pivotal role in regulation of diverse cellular processes.In particular,lncRNAs can serve as master gene regulators at transcriptional and posttranscriptional levels,leading to tumorigenesis.In this review,we discuss latest developments in lncRNA-meditated gene expression at the post-transcriptional level,including gene splicing,mRNA stability,protein stability and nuclear trafficking.
基金supported by the National Natural Science Foundation of China (grants 91540203 and 31788103 to X.C.,31771872 to X.S.)The National Key Research and Development Program of China (2016YFD0100904)+3 种基金the Genetically Modified Breeding Major Projects (grant no.2016ZX08009001 -005 to X.S.)the Key Research Program of Frontier Sciences Chinese Academy of Sciences (QYZDY-SSWSMC022 to X.C.)Strategic Priority Research Program of Chinese Academy of Sciences (XDB27030201 to X.C.)the State Key Laboratory of Plant Genomics.
文摘In plants,microRNA (miRNA) functions in the post-transcriptional repression of target mRNAs have been well explored.However,the mechanisms regulating the accumulation of miRNAs remain poorly under.stood.Here,we report that distinct mechanisms regulate accumulation of a monocot-specific miRNA,rice (Oryza sativa) miR528.At the transcriptional level,miR528 accumulated to higher levels in older plants than in young seedlings and exhibited aging-modulated gradual accumulation and diurnal rhythms in leaves;at the post-transcriptional level,aging also modulated miR528 levels by enhancing pri-miR528 alter.native splicing.We found that miR528 promotes rice flowering under long-day conditions by targeting RED AND FAR-RED INSENSITIVE2 (OsRFI2).Moreover,natural variations in the MIR528 promoter region caused differences in miR528 expression among rice varieties,which are correlated with their different binding affinities with the transcription factor OsSPL9 that activates the expression of miR528.Taken together,our findings reveal rice plants have evolved sophisticated modes fine-tuning miR528 levels and provide insight into the mechanisms that regulate MIRNA expression in plants.
基金Supported by Cancer projects in the C télab are funded through the Cancer Research Society,Canadian Research Institutes of Health Research and Canadian Breast Cancer Foundation
文摘Protein arginine methyltransferases(PRMTs) catalyze the methylation of a variety of protein substrates, many of which have been linked to the development, progression and aggressiveness of different types of cancer. Moreover, aberrant expression of PRMTs has been observed in several cancer types. While the link between PRMTs and cancer is a relatively new area of interest, the functional implications documented thus far warrant further investigations into its therapeutic potential. However, the expression of these enzymes and the regulation of their activity in cancer are still significantly understudied. Currently there are nine main members of the PRMT family. Further, the existence of alternatively spliced isoforms for several of these family members provides an additional layer of complexity. Specifically, PRMT1, PRMT2, CARM1 and PRMT7 have been shown to have alternative isoforms and others may be currently unrealized. Our knowledge with respect to the relative expression and the specific functions of these isoforms is largely lacking and needs attention. Here we present a review of the current knowledge of theknown alternative PRMT isoforms and provide a rationale for how they may impact on cancer and represent potentially useful targets for the development of novel therapeutic strategies.
文摘从福建主栽品种屏优一号PY分离到2个不能正常出菇的单孢菌株PYd21、PYd15,二者配对杂交得到可正常出菇异核菌株H15-21。用solexa测序技术对PYd21基因组从头测序(de novo sequencing),对PYd15、PYd21、H15-21的菌丝体分别进行数字基因表达谱测序,对8个样品(PYd15、PYd21、H15-21的菌丝体,H15-21出菇后的原基、钮扣期菌柄、蛋形期菌柄、伸长期菌柄和成熟期菌柄)mRNA等量混合物进行转录组测序。克隆测序了PYd21与PYd15中磷酸果糖激酶(PFK)基因,比对结果表明二者序列一致。利用转录组数据对PFK基因结构进行分析,结果表明:该基因全长3,494bp,有12个外显子、11个内含子;开放阅读框(ORF)全长2,457bp,编码818个氨基酸,5′端非翻译区(5′UTR)长度281bp,3′端非翻译区(3′UTR)全长103bp;RNA在加工过程中存在1种可变剪切类型、6个可变剪切位点。数字基因表达谱分析结果表明,PFK基因在PYd21、PYd15及H15-21中的标准表达量分别为71.08、120.61、251.85(transcriptper million cleantags,TPM),表达量依次升高,与菌丝生长速度显著正相关。并且异核菌株H15-21表达量高于2个单孢菌株之和,基因表达存在协同增效作用。采用实时荧光定量PCR技术对基因表达量进行验证,表达谱数据切实可靠。
