OBJECTIVE： To investigate the effect of Gubenfangxiao decoction （GBFXD） on respiratory-syncytial-virus （RSV） -induced asthma and the expression of asthma susceptibility gene, orosomucoid 1-1ike protein 3 （ORMDL3...OBJECTIVE： To investigate the effect of Gubenfangxiao decoction （GBFXD） on respiratory-syncytial-virus （RSV） -induced asthma and the expression of asthma susceptibility gene, orosomucoid 1-1ike protein 3 （ORMDL3） in mice. METHODS： Seventy-two female BALB/c mice were randomly assigned to normal, model, GBFXD high dose, GBFXD moderate dose, GBFXD low dose and montelukast groups. An asthma model was induced via intraperitoneal injection and aerosol inhalation of ovalbumin （OVA） and repeated intranasal instillation of RSV in all mice, except those in the normal group. All treatments were administered at the first onset of asthma （within 8 weeks of model establishment） and the mice were euthanized after 28 days of treatment. The levels of transforming growth factor-β （TGF-β） and interleukin-6 （IL-6） in bronchoalveolar lavage fluid （BALF） of the mice were measured and the expression of asthma sus- ceptibility gene ORMDL3 in lung tissue was deter- mined using real-time polymerase chain reaction （RT-PCR） and western blotting. RESULTS： Expression of ORMDL3 and levels of TGF-β and IL-6 were significantly higher in the mod- el group （P 〈 0.05, P 〈 0.01） compared with the normal mice. Levels of ORMDL3, TGF-β and IL-6 were significantly lower in all three GBFXD treated groups （P 〈 0.05） compared with the model group. However, the levels in the GBFXD treatment groups did not differ significantly from the montelukast group. CONCLUSION： GBFXD had a therapeutic effect in this experimental model. The functional mechanism of GBFXD may involve multiple factors, including alleviation of airway inflammation, down-regulation of asthma susceptibility gene ORMDL3 and inhibition of airway remodeling.展开更多
Recent damages to the box-like structures caused by wave slamming have made it necessary to study the impact problems of this kind of structure. This paper showed findings from numerical simulations of the rigid/elast...Recent damages to the box-like structures caused by wave slamming have made it necessary to study the impact problems of this kind of structure. This paper showed findings from numerical simulations of the rigid/elastic structures, aiming to gain insights into the characteristics of the problem. The results of the rigid cases showed the significance of air compressibility during the impact process, while the slamming phenomena became quite different without the effect. In the elastic cases, the trapped air made the structure vibrate at frequencies much smaller than its eigenfrequencies. Besides, the structural deformation made it easy for the trapped air to escape outwards, which weakened the air cushioning effect, especially at high impact velocities. The above analysis gives the results when the structural symmetry axis was vertical to the water(vertical impacts). In addition, the results were given when the axis was oblique to the water(oblique impacts). Compared with the vertical cases, the impact phenomena and structural response showed asymmetry. This work used the computational fluid dynamics(CFD) method to describe fluid motion and the finite element method(FEM) for the deformable structure. A two-way coupling approach was used to deal with the fluid-structure interaction in the elastic cases.展开更多
基金Supported by Natural Science Foundation of China(Effect of Gubenfangxiao Decoction on the Expression of Asthma Susceptibility Gene ORMDL3 and ADAM33 and its Signaling pathway,No.81173209)
文摘OBJECTIVE： To investigate the effect of Gubenfangxiao decoction （GBFXD） on respiratory-syncytial-virus （RSV） -induced asthma and the expression of asthma susceptibility gene, orosomucoid 1-1ike protein 3 （ORMDL3） in mice. METHODS： Seventy-two female BALB/c mice were randomly assigned to normal, model, GBFXD high dose, GBFXD moderate dose, GBFXD low dose and montelukast groups. An asthma model was induced via intraperitoneal injection and aerosol inhalation of ovalbumin （OVA） and repeated intranasal instillation of RSV in all mice, except those in the normal group. All treatments were administered at the first onset of asthma （within 8 weeks of model establishment） and the mice were euthanized after 28 days of treatment. The levels of transforming growth factor-β （TGF-β） and interleukin-6 （IL-6） in bronchoalveolar lavage fluid （BALF） of the mice were measured and the expression of asthma sus- ceptibility gene ORMDL3 in lung tissue was deter- mined using real-time polymerase chain reaction （RT-PCR） and western blotting. RESULTS： Expression of ORMDL3 and levels of TGF-β and IL-6 were significantly higher in the mod- el group （P 〈 0.05, P 〈 0.01） compared with the normal mice. Levels of ORMDL3, TGF-β and IL-6 were significantly lower in all three GBFXD treated groups （P 〈 0.05） compared with the model group. However, the levels in the GBFXD treatment groups did not differ significantly from the montelukast group. CONCLUSION： GBFXD had a therapeutic effect in this experimental model. The functional mechanism of GBFXD may involve multiple factors, including alleviation of airway inflammation, down-regulation of asthma susceptibility gene ORMDL3 and inhibition of airway remodeling.
基金financially supported by the National Key Research and Development Program of China (Grant No. 2019YFC1407700)。
文摘Recent damages to the box-like structures caused by wave slamming have made it necessary to study the impact problems of this kind of structure. This paper showed findings from numerical simulations of the rigid/elastic structures, aiming to gain insights into the characteristics of the problem. The results of the rigid cases showed the significance of air compressibility during the impact process, while the slamming phenomena became quite different without the effect. In the elastic cases, the trapped air made the structure vibrate at frequencies much smaller than its eigenfrequencies. Besides, the structural deformation made it easy for the trapped air to escape outwards, which weakened the air cushioning effect, especially at high impact velocities. The above analysis gives the results when the structural symmetry axis was vertical to the water(vertical impacts). In addition, the results were given when the axis was oblique to the water(oblique impacts). Compared with the vertical cases, the impact phenomena and structural response showed asymmetry. This work used the computational fluid dynamics(CFD) method to describe fluid motion and the finite element method(FEM) for the deformable structure. A two-way coupling approach was used to deal with the fluid-structure interaction in the elastic cases.
