目的对老年人自我护理能力量表(Self-care Ability Scale for the Elderly,SASE)进行汉化,并检测其信效度。方法采用分层随机抽样法对410例社区老年人和方便取样法对210例老年住院患者进行调查,运用SPSS17.0和AMOS 17.0软件对数据进行...目的对老年人自我护理能力量表(Self-care Ability Scale for the Elderly,SASE)进行汉化,并检测其信效度。方法采用分层随机抽样法对410例社区老年人和方便取样法对210例老年住院患者进行调查,运用SPSS17.0和AMOS 17.0软件对数据进行统计学分析。结果 SASE的内容效度指数为0.94,探索性因子分析得到3个公因子,累计方差贡献率为73.62%,验证性因子分析得到卡方/自由度(χ2/df)=2.61、残差均方和平方根(RMR)=0.02、渐进残差均方和平方根(RMSEA)=0.07、拟合优度指数(GFI)=0.95、调整拟合优度指数(AGFI)=0.92、非规范拟合指数(TLI)=0.93、增值拟合指数(IFI)=0.91、比较拟合指数(CFI)=0.94、简约拟合指数(PGFI)=0.62,均达到适配(拟合)标准;SASE的Cronbach'sα系数为0.82,重测信度为0.82。结论汉化版SASE信效度良好,可用于评价我国老年人自护能力的状况。展开更多
目的对2010-2016年发表的有关中国人骨质疏松症流行病学研究数据进行汇总,运用Meta分析方法汇总分析中国老年人骨质疏松症患病率情况。方法检索Pub Med、EMbase、Web of Science、Ovid、万方数据、中国知网及VIP中的英文和中文文献,提...目的对2010-2016年发表的有关中国人骨质疏松症流行病学研究数据进行汇总,运用Meta分析方法汇总分析中国老年人骨质疏松症患病率情况。方法检索Pub Med、EMbase、Web of Science、Ovid、万方数据、中国知网及VIP中的英文和中文文献,提取纳入研究的特征信息。数据分析采用R3.3.0软件,检验异质性,根据异质性结果选择相应的效应模型对提取数据进行加权定量合并。结果共纳入33项研究,样本总量30526例。33项研究存在异质性,按照随机效应模型计算中国60岁以上人群骨质疏松症总体患病率为36%,其中男性为23%,女性为49%,差异有统计学意义。结论骨质疏松症是中国老年人高发疾病,开展骨质疏松宣传防治工作意义重大。展开更多
AIM: To identify the property of dendritic cells (DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS: Twenty patients with persistent HBV infection were included in this study, 1...AIM: To identify the property of dendritic cells (DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS: Twenty patients with persistent HBV infection were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GM-CSF, IL-4, FLt-3,TNF-alpha and 100mL.L(-1 )of fetal calf serum for a total of 10-12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation, mytomycin C-treated DC were incubated with allogenic T cells. RESULTS: A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLA-DR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0.84 for CD86,P【0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCs simultaneously increased compared with healthy subjects (86 +/- 15 vs 170 +/- 22 micromol.L(-1), P 【0.05). CONCLUSION: The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV.展开更多
文摘目的对老年人自我护理能力量表(Self-care Ability Scale for the Elderly,SASE)进行汉化,并检测其信效度。方法采用分层随机抽样法对410例社区老年人和方便取样法对210例老年住院患者进行调查,运用SPSS17.0和AMOS 17.0软件对数据进行统计学分析。结果 SASE的内容效度指数为0.94,探索性因子分析得到3个公因子,累计方差贡献率为73.62%,验证性因子分析得到卡方/自由度(χ2/df)=2.61、残差均方和平方根(RMR)=0.02、渐进残差均方和平方根(RMSEA)=0.07、拟合优度指数(GFI)=0.95、调整拟合优度指数(AGFI)=0.92、非规范拟合指数(TLI)=0.93、增值拟合指数(IFI)=0.91、比较拟合指数(CFI)=0.94、简约拟合指数(PGFI)=0.62,均达到适配(拟合)标准;SASE的Cronbach'sα系数为0.82,重测信度为0.82。结论汉化版SASE信效度良好,可用于评价我国老年人自护能力的状况。
文摘目的对2010-2016年发表的有关中国人骨质疏松症流行病学研究数据进行汇总,运用Meta分析方法汇总分析中国老年人骨质疏松症患病率情况。方法检索Pub Med、EMbase、Web of Science、Ovid、万方数据、中国知网及VIP中的英文和中文文献,提取纳入研究的特征信息。数据分析采用R3.3.0软件,检验异质性,根据异质性结果选择相应的效应模型对提取数据进行加权定量合并。结果共纳入33项研究,样本总量30526例。33项研究存在异质性,按照随机效应模型计算中国60岁以上人群骨质疏松症总体患病率为36%,其中男性为23%,女性为49%,差异有统计学意义。结论骨质疏松症是中国老年人高发疾病,开展骨质疏松宣传防治工作意义重大。
基金National Natural Science Foundation of China,No.39970831.
文摘AIM: To identify the property of dendritic cells (DCs) of peripheral blood monocytes (PBMC) in patients with chronic HBV infection. METHODS: Twenty patients with persistent HBV infection were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GM-CSF, IL-4, FLt-3,TNF-alpha and 100mL.L(-1 )of fetal calf serum for a total of 10-12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation, mytomycin C-treated DC were incubated with allogenic T cells. RESULTS: A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLA-DR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0.84 for CD86,P【0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCs simultaneously increased compared with healthy subjects (86 +/- 15 vs 170 +/- 22 micromol.L(-1), P 【0.05). CONCLUSION: The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV.
