The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization （FISH） with 18S-28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing in...The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization （FISH） with 18S-28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentrie chromosome 10. After silverstaining, nucleolus organizer regions （NORs） could be observed on the telomere of the short arm of chromosome 10. However, one metaphase spread displayed an additional silver spot on the short arm of subtelocentrie chromosome 12.展开更多
基金work was mainly supported by the ＂863＂ Hi-Tech Research and Development Program of China under contract No. 2005AA603220the National Natural Science Foundation of China under contract No. 30300268.
文摘The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization （FISH） with 18S-28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentrie chromosome 10. After silverstaining, nucleolus organizer regions （NORs） could be observed on the telomere of the short arm of chromosome 10. However, one metaphase spread displayed an additional silver spot on the short arm of subtelocentrie chromosome 12.
