Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and accept...Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual.展开更多
目的探析日常生活能力评定量表在精神科病房分级护理管理中的应用效果。方法选取我院精神科病房178例患者作为研究对象,随机分为观察组与对照组各89例;其中对照组由护理人员遵照医生开具的护理级别医嘱进行护理,观察组由护理人员通过观...目的探析日常生活能力评定量表在精神科病房分级护理管理中的应用效果。方法选取我院精神科病房178例患者作为研究对象,随机分为观察组与对照组各89例;其中对照组由护理人员遵照医生开具的护理级别医嘱进行护理,观察组由护理人员通过观察患者病情,参照日常生活能力评定量表(Activity of daily living scale,ADL)制定护理分级从而进行护理。比较分析两组患者的基础护理质量(皮肤与口腔卫生、衣着卫生、饮食护理、排泄护理、睡眠护理)、护理满意度以及住院期间不良事件发生率。结果观察组的皮肤与口腔卫生、衣着卫生、饮食护理、排泄护理、睡眠护理评分均显著高于对照组(均P<0.001);观察组的护理满意度为96.6%,显著高于对照组85.4%(P<0.001);观察组的不良事件发生率为7.9%,显著低于对照组20.2%(P=0.033)。结论通过结合ADL确定精神科病房患者的护理分级可有效提高基础护理质量、护理满意度,并有效减少患者住院期间的不良事件发生率,同时增加患者的依从性,具有良好的临床实用价值。展开更多
基金This work was supported by publicly donated Intestine Initiative FoundationPrimary Research&Development Plan of Jiangsu Province(BE2018751)+1 种基金Jiangsu Provincial Medical Innovation Team(Zhang F),National Natural Science Foundation of China(81600417,81670495 and 81873548)China National Center for Clinical Research of Digestive Diseases(201502026).
文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual.
文摘目的探析日常生活能力评定量表在精神科病房分级护理管理中的应用效果。方法选取我院精神科病房178例患者作为研究对象,随机分为观察组与对照组各89例;其中对照组由护理人员遵照医生开具的护理级别医嘱进行护理,观察组由护理人员通过观察患者病情,参照日常生活能力评定量表(Activity of daily living scale,ADL)制定护理分级从而进行护理。比较分析两组患者的基础护理质量(皮肤与口腔卫生、衣着卫生、饮食护理、排泄护理、睡眠护理)、护理满意度以及住院期间不良事件发生率。结果观察组的皮肤与口腔卫生、衣着卫生、饮食护理、排泄护理、睡眠护理评分均显著高于对照组(均P<0.001);观察组的护理满意度为96.6%,显著高于对照组85.4%(P<0.001);观察组的不良事件发生率为7.9%,显著低于对照组20.2%(P=0.033)。结论通过结合ADL确定精神科病房患者的护理分级可有效提高基础护理质量、护理满意度,并有效减少患者住院期间的不良事件发生率,同时增加患者的依从性,具有良好的临床实用价值。
