Objective： This study observed attenuating effect of hydroxysafflor yellow A （HSYA）, an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide （LPS）-induced endothelium inflammat...Objective： This study observed attenuating effect of hydroxysafflor yellow A （HSYA）, an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide （LPS）-induced endothelium inflammatory injury. Methods： Eahy926 human endothelium cell （EC） line was used; thiazolyl blue tetrazolium bromide （MTT） test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor- κB （NF- κ B） p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase （MAPKs） and NF- κ B activation. Reverse transcription polymerase chain reaction （RT-PCR） method was applied to observe intercellular cell adhesion molecule-1 （ICAM-1） and E-selectin mRNA level; EC surface ICAM-1 expression was measured with flow cytometry and leukocyte adhesion to EC was assayed with Rose Bengal spectrophotometry technology. Results： HSYA protected EC viability against LPS-induced injury （P〈0.05）. LPS-induced NF- κ B p65 subunit DNA binding （P〈0.01） and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α （I κ B α） phosphorylation was inhibited by HSYA. HSYA attenuated LPS triggered ICAM-1 and E-selectin mRNA levels elevation and phosphorylation of p38 MAPK or c-Jun N-terminal kinase MAPK. HSYA also inhibited LPS-induced cell surface ICAM-1 protein expression （P〈0.01） and leukocyte adhesion to EC （P〈0.05）. Conclusion： HSYA is effective to protect LPS-induced high expression of endothelium adhesive molecule and inflammatory signal transduction.展开更多
Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and de...Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and development of gastric cancer. Methods RT-PCR was used to detect mRNA of CD 44V and CD 44S in 10 gastric normal mucosa,8 gastric precancerous mucosa,10 tissue adjacent to cancer,40 gastric carcinoma (including metastatic focus) with different pathological features.Results All gastric carcinomas and non-cancerous tissues expressed CD 44S ,but CD 44V expression rate were 0%, 12.5%,20% and 72.5% ,respectively, in normal gastric mucosa, precancerous tissue,tissue adjacent to cancer,carcinoma. Cancer invasion limited to submucosal layer showed lower CD 44V expression rate than invasion extended beyond the muscle layer (P<0.05). Samples with lymph node metastasis showed higher CD 44V expression rate than samples without lymph node metastasis,meanwhile, there was significant correlation between metastasis limited to N1 and beyond N1 (P<0.05). There was no relationship between CD 44V expression and Lauren's type, TNM stage as well (P>0.05). Conclusion There is a close relation between CD 44V and genesis and development of gastric cancer. CD 44 gene variant splicing is an early genetic affair in gastric cancer.It could be a diagnostic tool to screen high-risk people for gastric carcinoma. CD 44V can be regard as a parameter in evaluating the potential of cancer cell invasion and metastasis ,as well as prognosis.展开更多
目的:探讨不同压强CO2气腹对胃癌细胞侵袭黏附能力的影响。方法:建立体外CO2气腹模型,选胃中分化腺癌株SGC-7901,分别暴露在6、9、12、15 mm Hg(1 mm Hg=0.133 k Pa)和常规细胞培养环境下各3 h。Real-time PCR法检测胃癌细胞中黏附侵袭...目的:探讨不同压强CO2气腹对胃癌细胞侵袭黏附能力的影响。方法:建立体外CO2气腹模型,选胃中分化腺癌株SGC-7901,分别暴露在6、9、12、15 mm Hg(1 mm Hg=0.133 k Pa)和常规细胞培养环境下各3 h。Real-time PCR法检测胃癌细胞中黏附侵袭分子E-cadherin、细胞黏附侵袭分子-1(ICAM-1)、基质金属蛋白酶-2(MMP-2)和细胞黏附分子CD44v6的表达水平;Westernblot检测胃癌细胞中E-cadherin、ICAM-1、MMP-2和CD44v6蛋白的表达水平。结果:随着CO2压强升高,在15 mm Hg时,E-cadherin的mRNA相对表达水平较对照组明显下降(P<0.05);MMP-2和CD44v6的mRNA相对表达水平较对照组明显上升(P<0.05);而ICAM-1 mRNA的相对表达水平也有上调趋势,但各组与对照组比较均无统计学差异。Western-blot检测结果显示蛋白的表达水平与基因检测结果一致。压强为15 mm Hg时,CD44v6和MMP-2的表达上调,E-cadherin的表达下调,具有统计学差异。结论:在压强不高于12 mm Hg时,不同压强的CO2气腹对胃癌细胞株的黏附侵袭能力无明显影响,并不增加肿瘤的转移几率。展开更多
基金Supported by National Natural Science Foundation of China(No.81270103)Natural Science Foundation of Beijing(No.7102025)
文摘Objective： This study observed attenuating effect of hydroxysafflor yellow A （HSYA）, an effective ingredient of aqueous extract of Carthamus tinctorius L, on lipopolysaccharide （LPS）-induced endothelium inflammatory injury. Methods： Eahy926 human endothelium cell （EC） line was used; thiazolyl blue tetrazolium bromide （MTT） test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor- κB （NF- κ B） p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase （MAPKs） and NF- κ B activation. Reverse transcription polymerase chain reaction （RT-PCR） method was applied to observe intercellular cell adhesion molecule-1 （ICAM-1） and E-selectin mRNA level; EC surface ICAM-1 expression was measured with flow cytometry and leukocyte adhesion to EC was assayed with Rose Bengal spectrophotometry technology. Results： HSYA protected EC viability against LPS-induced injury （P〈0.05）. LPS-induced NF- κ B p65 subunit DNA binding （P〈0.01） and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α （I κ B α） phosphorylation was inhibited by HSYA. HSYA attenuated LPS triggered ICAM-1 and E-selectin mRNA levels elevation and phosphorylation of p38 MAPK or c-Jun N-terminal kinase MAPK. HSYA also inhibited LPS-induced cell surface ICAM-1 protein expression （P〈0.01） and leukocyte adhesion to EC （P〈0.05）. Conclusion： HSYA is effective to protect LPS-induced high expression of endothelium adhesive molecule and inflammatory signal transduction.
文摘Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and development of gastric cancer. Methods RT-PCR was used to detect mRNA of CD 44V and CD 44S in 10 gastric normal mucosa,8 gastric precancerous mucosa,10 tissue adjacent to cancer,40 gastric carcinoma (including metastatic focus) with different pathological features.Results All gastric carcinomas and non-cancerous tissues expressed CD 44S ,but CD 44V expression rate were 0%, 12.5%,20% and 72.5% ,respectively, in normal gastric mucosa, precancerous tissue,tissue adjacent to cancer,carcinoma. Cancer invasion limited to submucosal layer showed lower CD 44V expression rate than invasion extended beyond the muscle layer (P<0.05). Samples with lymph node metastasis showed higher CD 44V expression rate than samples without lymph node metastasis,meanwhile, there was significant correlation between metastasis limited to N1 and beyond N1 (P<0.05). There was no relationship between CD 44V expression and Lauren's type, TNM stage as well (P>0.05). Conclusion There is a close relation between CD 44V and genesis and development of gastric cancer. CD 44 gene variant splicing is an early genetic affair in gastric cancer.It could be a diagnostic tool to screen high-risk people for gastric carcinoma. CD 44V can be regard as a parameter in evaluating the potential of cancer cell invasion and metastasis ,as well as prognosis.
文摘目的:探讨不同压强CO2气腹对胃癌细胞侵袭黏附能力的影响。方法:建立体外CO2气腹模型,选胃中分化腺癌株SGC-7901,分别暴露在6、9、12、15 mm Hg(1 mm Hg=0.133 k Pa)和常规细胞培养环境下各3 h。Real-time PCR法检测胃癌细胞中黏附侵袭分子E-cadherin、细胞黏附侵袭分子-1(ICAM-1)、基质金属蛋白酶-2(MMP-2)和细胞黏附分子CD44v6的表达水平;Westernblot检测胃癌细胞中E-cadherin、ICAM-1、MMP-2和CD44v6蛋白的表达水平。结果:随着CO2压强升高,在15 mm Hg时,E-cadherin的mRNA相对表达水平较对照组明显下降(P<0.05);MMP-2和CD44v6的mRNA相对表达水平较对照组明显上升(P<0.05);而ICAM-1 mRNA的相对表达水平也有上调趋势,但各组与对照组比较均无统计学差异。Western-blot检测结果显示蛋白的表达水平与基因检测结果一致。压强为15 mm Hg时,CD44v6和MMP-2的表达上调,E-cadherin的表达下调,具有统计学差异。结论:在压强不高于12 mm Hg时,不同压强的CO2气腹对胃癌细胞株的黏附侵袭能力无明显影响,并不增加肿瘤的转移几率。
