Black rockfish Sebastes schlegelii juveniles (30-40 mm total length) were immersed in a range of calcein (CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S (ARS) solutions at concen...Black rockfish Sebastes schlegelii juveniles (30-40 mm total length) were immersed in a range of calcein (CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S (ARS) solutions at concentrations ranging from 100 to 500 mg/L in filtered seawater (salinity 30) for 24 h. Fluorescent marks were detected in otoliths (sagittae, asteriscus), scales, fin rays (dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines (dorsal, ventral, and anal fin spines) after a 60-d growth experiment. With the exception of 50-100 rng/L CAL, acceptable marks were produced in the otoliths and fin spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fin spines offish immersed in 200 500 mg/L, 300-500 rag/L, and 200-500 mg/LARS, respectively. Scales and fin rays had acceptable marks at much higher concentrations (≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL,≥200 mg/L ARS for fin rays). The mark quality was highest (i.e., acceptable marks were observed in all sampled structures after immersion marking) in fish immersed in 150-250 mg/L CAL or 300-500 mg/LARS. In addition, there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking (P〉0.05).展开更多
Biogeochemical cyclic activity of the ars (arsenic resistance system) operon is arsB influx/effux encoded by the ecological of Pseudomonas putida.This suggests that studying arsenite-oxidizing bacteria may lead to a b...Biogeochemical cyclic activity of the ars (arsenic resistance system) operon is arsB influx/effux encoded by the ecological of Pseudomonas putida.This suggests that studying arsenite-oxidizing bacteria may lead to a better understanding of molecular geomicrobiology,which can be applied to the bioremediation of arsenic-contaminated mines.This is the first report in which multiple arsB-binding mechanisms have been used on indigenous bacteria.In ArsB (strains OS-5; ABB83931; OS-19; ABB04282 and RW-28; ABB88574...展开更多
round-the-clock solar observations with full-disk coverage of vector magnetograms and multi-wavelength images demonstrate that solar active regions(ARs) are ultimately connected with magnetic field. Often two or more ...round-the-clock solar observations with full-disk coverage of vector magnetograms and multi-wavelength images demonstrate that solar active regions(ARs) are ultimately connected with magnetic field. Often two or more ARs are clustered, creating a favorable magnetic environment for the onset of coronal mass ejections(CMEs). In this work, we describe a new type of magnetic complex: cluster of solar ARs. An AR cluster is referred to as the close connection of two or more ARs which are located in nearly the same latitude and a narrow span of longitude. We illustrate three examples of AR clusters, each of which has two ARs connected and formed a common dome of magnetic flux system. They are clusters of NOAA(i.e., National Oceanic and Atmospheric Administration) ARs 11226 & 11227, 11429 & 11430, and 11525 & 11524. In these AR clusters, CME initiations were often tied to the instability of the magnetic structures connecting two partner ARs, in the form of inter-connecting loops and/or channeling filaments between the two ARs. We show the evidence that, at least, some of the flare/CMEs in an AR cluster are not a phenomenon of a single AR, but the result of magnetic interaction in the whole AR cluster. The observations shed new light on understanding the mechanism(s) of solar activity. Instead of the simple bipolar topology as suggested by the so-called standard flare model, a multi-bipolar magnetic topology is more common to host the violent solar activity in solar atmosphere.展开更多
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201003068)the National Natural Science Foundation of China(Nos.31172447,41176117)
文摘Black rockfish Sebastes schlegelii juveniles (30-40 mm total length) were immersed in a range of calcein (CAL) solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S (ARS) solutions at concentrations ranging from 100 to 500 mg/L in filtered seawater (salinity 30) for 24 h. Fluorescent marks were detected in otoliths (sagittae, asteriscus), scales, fin rays (dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines (dorsal, ventral, and anal fin spines) after a 60-d growth experiment. With the exception of 50-100 rng/L CAL, acceptable marks were produced in the otoliths and fin spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fin spines offish immersed in 200 500 mg/L, 300-500 rag/L, and 200-500 mg/LARS, respectively. Scales and fin rays had acceptable marks at much higher concentrations (≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL,≥200 mg/L ARS for fin rays). The mark quality was highest (i.e., acceptable marks were observed in all sampled structures after immersion marking) in fish immersed in 150-250 mg/L CAL or 300-500 mg/LARS. In addition, there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking (P〉0.05).
基金the Korea Science and Engineering Foundation (KOSEF) through the National Research Lab.Program funded by the Korean Ministry of Science and Technology (No.M10300000298-06J0000-29810).
文摘Biogeochemical cyclic activity of the ars (arsenic resistance system) operon is arsB influx/effux encoded by the ecological of Pseudomonas putida.This suggests that studying arsenite-oxidizing bacteria may lead to a better understanding of molecular geomicrobiology,which can be applied to the bioremediation of arsenic-contaminated mines.This is the first report in which multiple arsB-binding mechanisms have been used on indigenous bacteria.In ArsB (strains OS-5; ABB83931; OS-19; ABB04282 and RW-28; ABB88574...
基金supported by the National Natural Science Foundation of China(Grant Nos.11221063,11373004,11322329,41404150,and 11303049)Ministry of Science and Technology(Grant No.2011CB811403)
文摘round-the-clock solar observations with full-disk coverage of vector magnetograms and multi-wavelength images demonstrate that solar active regions(ARs) are ultimately connected with magnetic field. Often two or more ARs are clustered, creating a favorable magnetic environment for the onset of coronal mass ejections(CMEs). In this work, we describe a new type of magnetic complex: cluster of solar ARs. An AR cluster is referred to as the close connection of two or more ARs which are located in nearly the same latitude and a narrow span of longitude. We illustrate three examples of AR clusters, each of which has two ARs connected and formed a common dome of magnetic flux system. They are clusters of NOAA(i.e., National Oceanic and Atmospheric Administration) ARs 11226 & 11227, 11429 & 11430, and 11525 & 11524. In these AR clusters, CME initiations were often tied to the instability of the magnetic structures connecting two partner ARs, in the form of inter-connecting loops and/or channeling filaments between the two ARs. We show the evidence that, at least, some of the flare/CMEs in an AR cluster are not a phenomenon of a single AR, but the result of magnetic interaction in the whole AR cluster. The observations shed new light on understanding the mechanism(s) of solar activity. Instead of the simple bipolar topology as suggested by the so-called standard flare model, a multi-bipolar magnetic topology is more common to host the violent solar activity in solar atmosphere.