In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quan...In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal standard.The expression of ArcA was undetectable until about 3 hours under standard culture conditions,but it was promptly and highly expressed in anaerobic culture.The results are consistent with ArcA being a potential virulence gene of Actinobacillus pleuropneumoniae,and likely playing an important role in pathogenesis caused by this organism.展开更多
The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper,...The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper, authors describe the Data Management System the DMS （Data Management System） developed by INGV （Istituto Nazionale di Geofisica e Vulcanologia） to support the brokering system GEOSS （Global Earth Observation System of Systems） adopted for the ARCA （Arctic Present Climate Change and Past Extreme Events） project. This DMS includes heterogeneous data that contributes to the ARCA objective （www.arcaproject.it） focusing on multi-parametric and multi-disciplinary studies on the mechanism （s） behind the release of large volumes of cold and fresh water from melting of ice caps. The DMS is accessible directly at the www.arca.rm.ingv.it, or through the IADC （Italian Arctic Data Center） at http：//arcticnode.dta.cnr.it/iadc/gi-portal/index.jsp that interoperates with the GEOSS brokering system （http：//www.geoportal.org0 making easy and fast the search of specific data set and its URL.展开更多
-The impact of several chemical poisons on the Arca granosa absorbing 45Ca was studied. The results showed that the absorption of 45Ca in the blood and the soft tissue by Arca granosa was inhibited by the inorganic co...-The impact of several chemical poisons on the Arca granosa absorbing 45Ca was studied. The results showed that the absorption of 45Ca in the blood and the soft tissue by Arca granosa was inhibited by the inorganic compounds of Cu, Zn, Pd, Cd, Hg and so on, among which the inhibition of Cd was the strongest, next was that of Cu and Hg. But the inhibition of the absorption of 45Ca in the shell of Arca granosa was little. The impact of three kinds of energy metabolism inhibitory substances on the absorption of 45Ca by the Area granosa showed that the absorption was an active transfer process.展开更多
基金supported by the Biotechnological Key Project of Sichuan Province,China(05NG020-016)The Yangtze River Scholar and Innovation Team develop Project of Ministry of Education,China(IRT0555-9)
文摘In the present study,mRNA levels of the ArcA gene of Actinobacillus pleuropneumoniae serotype1 were measured during response to stress by growing under anaerobic conditions.Expression levels were measured by semi-quantitative RT-PCR using the housekeeping gene recF as an internal standard.The expression of ArcA was undetectable until about 3 hours under standard culture conditions,but it was promptly and highly expressed in anaerobic culture.The results are consistent with ArcA being a potential virulence gene of Actinobacillus pleuropneumoniae,and likely playing an important role in pathogenesis caused by this organism.
文摘The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper, authors describe the Data Management System the DMS （Data Management System） developed by INGV （Istituto Nazionale di Geofisica e Vulcanologia） to support the brokering system GEOSS （Global Earth Observation System of Systems） adopted for the ARCA （Arctic Present Climate Change and Past Extreme Events） project. This DMS includes heterogeneous data that contributes to the ARCA objective （www.arcaproject.it） focusing on multi-parametric and multi-disciplinary studies on the mechanism （s） behind the release of large volumes of cold and fresh water from melting of ice caps. The DMS is accessible directly at the www.arca.rm.ingv.it, or through the IADC （Italian Arctic Data Center） at http：//arcticnode.dta.cnr.it/iadc/gi-portal/index.jsp that interoperates with the GEOSS brokering system （http：//www.geoportal.org0 making easy and fast the search of specific data set and its URL.
文摘-The impact of several chemical poisons on the Arca granosa absorbing 45Ca was studied. The results showed that the absorption of 45Ca in the blood and the soft tissue by Arca granosa was inhibited by the inorganic compounds of Cu, Zn, Pd, Cd, Hg and so on, among which the inhibition of Cd was the strongest, next was that of Cu and Hg. But the inhibition of the absorption of 45Ca in the shell of Arca granosa was little. The impact of three kinds of energy metabolism inhibitory substances on the absorption of 45Ca by the Area granosa showed that the absorption was an active transfer process.
