Ventricular remodeling （VR） after myocardial infarction （MI） makes a full impact on left ventricular dilation and dysfunction, severe arrhythmias and even sudden death. Thus it is very interesting and instructive ...Ventricular remodeling （VR） after myocardial infarction （MI） makes a full impact on left ventricular dilation and dysfunction, severe arrhythmias and even sudden death. Thus it is very interesting and instructive to study the underlying regulatory mechanism for VR. Recently, evidenceI suggests that tumor necrosis factor-α （TNF-α） activity can independently influence VR, and aggravate myocardial dysfunction and cell death in the ventricle. The activation of pro-TNF〈t is adjusted by a disintegrin metalloproteinase （ADAM） 10 and ADAM17, the latter might take part in extracellular matrix （ECM） modulation in the borderline region of cardiac infarction.2 However, little is known about the relationship between ADAMsl0, 17 expressions and TNF-α activity in the process of VR after MI. The present study tested the hypothesis in rats that the interaction between ADAMsl0, 17 expressions and TNF-α activity was a contributory mechanism for VR of the healing myocardium, and metoprolol treatment might ameliorate VR inhibiting the mechanism.展开更多
目的探讨ADAM17(a disintegrin and metalloproteinase 17,ADAM17)mRNA在孤立性大肝癌(SLHCC)中的表达及其与病理特征的关系。方法采用逆转录多聚酶链式反应(RT-PCR)半定量检测8例SLHCC、12例小肝癌(SHCC),11例结节性肝癌(NHCC)以及相...目的探讨ADAM17(a disintegrin and metalloproteinase 17,ADAM17)mRNA在孤立性大肝癌(SLHCC)中的表达及其与病理特征的关系。方法采用逆转录多聚酶链式反应(RT-PCR)半定量检测8例SLHCC、12例小肝癌(SHCC),11例结节性肝癌(NHCC)以及相应癌旁肝组织中ADAM17mRNA的表达。结果NHCC的静脉浸润(83·3%)明显多于SLHCC和SHCC,其分化程度也较SLHCC和SHCC差(P<0·05),而比较SLHCC和SHCC的病理特征未见明显差异。77·4%的肝癌组织中可见ADAM17mRNA表达,明显高于癌旁肝组织(35·5%)。NHCC中ADAM17mRNA的表达高于SLHCC和SHCC(P<0·05)。分化较差的肝癌中ADAM17mRNA表达高于分化较好的肝癌(P<0·05)。结论ADAM17mRNA的表达与肝癌的分化程度密切相关。而SLHCC表达ADAM17mRNA相对较低可能与SLHCC相对良好的分子病理特征有关。展开更多
基金This work was supported by the grants from the National Natural Science Foundation of Chian(No.30470687)Heilongjiang Doctorate Foundation grant(No.SCX2005020)
文摘Ventricular remodeling （VR） after myocardial infarction （MI） makes a full impact on left ventricular dilation and dysfunction, severe arrhythmias and even sudden death. Thus it is very interesting and instructive to study the underlying regulatory mechanism for VR. Recently, evidenceI suggests that tumor necrosis factor-α （TNF-α） activity can independently influence VR, and aggravate myocardial dysfunction and cell death in the ventricle. The activation of pro-TNF〈t is adjusted by a disintegrin metalloproteinase （ADAM） 10 and ADAM17, the latter might take part in extracellular matrix （ECM） modulation in the borderline region of cardiac infarction.2 However, little is known about the relationship between ADAMsl0, 17 expressions and TNF-α activity in the process of VR after MI. The present study tested the hypothesis in rats that the interaction between ADAMsl0, 17 expressions and TNF-α activity was a contributory mechanism for VR of the healing myocardium, and metoprolol treatment might ameliorate VR inhibiting the mechanism.
文摘目的探讨ADAM17(a disintegrin and metalloproteinase 17,ADAM17)mRNA在孤立性大肝癌(SLHCC)中的表达及其与病理特征的关系。方法采用逆转录多聚酶链式反应(RT-PCR)半定量检测8例SLHCC、12例小肝癌(SHCC),11例结节性肝癌(NHCC)以及相应癌旁肝组织中ADAM17mRNA的表达。结果NHCC的静脉浸润(83·3%)明显多于SLHCC和SHCC,其分化程度也较SLHCC和SHCC差(P<0·05),而比较SLHCC和SHCC的病理特征未见明显差异。77·4%的肝癌组织中可见ADAM17mRNA表达,明显高于癌旁肝组织(35·5%)。NHCC中ADAM17mRNA的表达高于SLHCC和SHCC(P<0·05)。分化较差的肝癌中ADAM17mRNA表达高于分化较好的肝癌(P<0·05)。结论ADAM17mRNA的表达与肝癌的分化程度密切相关。而SLHCC表达ADAM17mRNA相对较低可能与SLHCC相对良好的分子病理特征有关。
