Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolic extract ofFicus sarmentosa var. henryi were assayed against Musca domestica adults. The chlor...Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolic extract ofFicus sarmentosa var. henryi were assayed against Musca domestica adults. The chloroform- and ethyl acetate- soluble fractions were the most active with 92.6 and 88.9% mortalities （24 h after treatment） respectively. Therefore, the two fractions were combined and four compounds, isolated from the fractions by activity-guided fractionation, were elucidated as 7-hydroxycoumarin, apigenin, eriodictyol, and quercetin by spectroscopic method and displayed excellent insecticidal activities against adults of M. domestica and 4th instar larva of Aedes albopictus. Among those, 7- hydroxycoumarin showed the strongest insecticidal activities with lethal concentrations （LC50） values of 72.13 μg g^-1 sugar and 4.87 μg mL^-1 （48 h after treatment） against the test insects respectively. The cytoxicities of these compounds on BTI-Tn-5B 1-4 cell were also investigated for the insecticidal mechanism and found that quercetin represented superior inhibitory activity with MTT assay and reactive oxygen species （ROS） against BTI-Tn-5B 1-4 cell, but slightly weaker than that of the positive control （azadirachtin） and significantly greater than the negative control （DMSO only）. Meanwhile, eriodictyol demonstrated the strongest effect on the mitochondrial membrane potentials （MMP）. In conclusion, based on their comparative toxicities to commercial insecticides and their cytotoxic effects, some of the compounds from the F. sarmentosa have potential as botanical insecticides.展开更多
目的:建立HPLC法测定参附强心丸中东莨菪内酯和7-羟基香豆素含量的方法。方法:采用二极管阵列检测器,色谱柱为Phenomenex Luna C_(18)(250mm×4.6mm,5μm);流动相为甲醇-0.5%冰乙酸水溶液,梯度洗脱;流速为1.0m L/min;检测波长为345n...目的:建立HPLC法测定参附强心丸中东莨菪内酯和7-羟基香豆素含量的方法。方法:采用二极管阵列检测器,色谱柱为Phenomenex Luna C_(18)(250mm×4.6mm,5μm);流动相为甲醇-0.5%冰乙酸水溶液,梯度洗脱;流速为1.0m L/min;检测波长为345nm。结果:东莨菪内酯线性范围为0.0760~0.3800μg(R2=0.999),平均回收率为104.54%,RSD为2.27%,重复性RSD为1.22%(n=6),精密度RSD为0.90%(n=6),稳定性RSD为0.91%;7-羟基香豆素线性范围为0.0300~0.1500μg(R^2=0.999),平均回收率为102.51%,RSD为1.04%,重复性RSD为5.08%(n=6),精密度RSD为0.91%(n=6),稳定性RSD为0.91%。结论:本研究所建方法稳定、可靠,可作为该制剂的质量控制方法。展开更多
基金support by the National Natural Science Foundation of China (30571235)
文摘Insecticidal activities of the petroleum ether-, chloroform-, ethyl acetate-, and water-soluble fractions of the methanolic extract ofFicus sarmentosa var. henryi were assayed against Musca domestica adults. The chloroform- and ethyl acetate- soluble fractions were the most active with 92.6 and 88.9% mortalities （24 h after treatment） respectively. Therefore, the two fractions were combined and four compounds, isolated from the fractions by activity-guided fractionation, were elucidated as 7-hydroxycoumarin, apigenin, eriodictyol, and quercetin by spectroscopic method and displayed excellent insecticidal activities against adults of M. domestica and 4th instar larva of Aedes albopictus. Among those, 7- hydroxycoumarin showed the strongest insecticidal activities with lethal concentrations （LC50） values of 72.13 μg g^-1 sugar and 4.87 μg mL^-1 （48 h after treatment） against the test insects respectively. The cytoxicities of these compounds on BTI-Tn-5B 1-4 cell were also investigated for the insecticidal mechanism and found that quercetin represented superior inhibitory activity with MTT assay and reactive oxygen species （ROS） against BTI-Tn-5B 1-4 cell, but slightly weaker than that of the positive control （azadirachtin） and significantly greater than the negative control （DMSO only）. Meanwhile, eriodictyol demonstrated the strongest effect on the mitochondrial membrane potentials （MMP）. In conclusion, based on their comparative toxicities to commercial insecticides and their cytotoxic effects, some of the compounds from the F. sarmentosa have potential as botanical insecticides.
文摘目的:建立HPLC法测定参附强心丸中东莨菪内酯和7-羟基香豆素含量的方法。方法:采用二极管阵列检测器,色谱柱为Phenomenex Luna C_(18)(250mm×4.6mm,5μm);流动相为甲醇-0.5%冰乙酸水溶液,梯度洗脱;流速为1.0m L/min;检测波长为345nm。结果:东莨菪内酯线性范围为0.0760~0.3800μg(R2=0.999),平均回收率为104.54%,RSD为2.27%,重复性RSD为1.22%(n=6),精密度RSD为0.90%(n=6),稳定性RSD为0.91%;7-羟基香豆素线性范围为0.0300~0.1500μg(R^2=0.999),平均回收率为102.51%,RSD为1.04%,重复性RSD为5.08%(n=6),精密度RSD为0.91%(n=6),稳定性RSD为0.91%。结论:本研究所建方法稳定、可靠,可作为该制剂的质量控制方法。
