本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提...本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提供相应依据。采用测序技术对498只清远麻鸡VIPR-1基因5′调控区进行多态性检测及基因型分析,利用最小二乘均数对VIPR-1基因5′调控区的多态位点与就巢性状进行相关分析,利用PHASE软件对多态位点进行单倍型分析。结果,VIPR-1基因5′调控区存在12个多态性位点,G-359T、G-266T、A-134G、A-94G、C-72G对18～43周龄的就巢持续天数影响达到显著水平(P<0.05),A-94G位点对18～54周龄的就巢率的影响达到显著水平(P<0.05);构建的单倍型对18～54周龄的就巢持续天数的影响达到极显著水平(P<0.01)。最小二乘分析结果表明,CCTGGGAAGCAG/TTGGGGAAGCAC型个体的就巢持续天数比其它单倍型个体极显著的延长(P<0.01)。结果表明,VIPR-1基因5′调控区(-496～-1bp)可能存在影响鸡就巢行为的分子标记,CCTGGGAAGCAG/TTGGGGAAGCAC型个体对鸡就巢性具有较大的遗传效应,可作为分子标记应用于家鸡就巢行为的筛选。展开更多
Myostatin is a negative regulator of skeletal muscle mass.The present studycloned the 5' regulatory region of porcine myostatin gene,screened its polymorphisms and analyzedtheir associations with early growth trai...Myostatin is a negative regulator of skeletal muscle mass.The present studycloned the 5' regulatory region of porcine myostatin gene,screened its polymorphisms and analyzedtheir associations with early growth traits in Yorkshire pigs.The results indicated that a fragmentlength polymorphism and a polymorphism concerning two nucleotide changes exist in the 5' regulatoryregion of porcine myostatin gene.At sites 435 and 447,allele A and allele B have the haplotypes ofA-G and G-A,respectively.The allelic frequency of B is 0.475 in Yorkshire pigs.No homozygous BBgenotype was detected in 9 Laiwu Black pigs.Allele B was found to have positive effect on bodyweight on day 21 (BW21) (P【0.01),body weight on day 28 (BW28) (P【0.05),body weight on day 70 (BW70)(P【0.05),average daily gain from birth to 21 d (ADG1) (P【0.01),average daily gain from birth to 28 d(ADG2) (P【0.05) and average daily gain from 21 d to 70 d (ADG3) (P【0.01),respectively.The additiveeffect of allele B on BW21,BW28,BW70,ADG1,ADG2 and ADG3 was 0.596±0.205 kg (P=0.0041),0.498±0.200kg (P=0.0136) 1.409±0.551 kg (P=0.0112),28.39±9.74 g P=0.0041),17.78±7.15 g (P=0.0136) and37.00±16.92 g (P=0.0304),respectively,whereas its effect on average daily gain from 28 d to 70 d(ADG4) was not significant (P】0.1),although BB individuals are superior in average daily gain to AAand AB.展开更多
文摘本研究旨在探索血管活性肠肽I型受体(Vasoacitve intestinal peptide type I receptor,VIPR-1)基因5′调控区(-496～-1bp)多态性及其单倍型效应对鸡就巢性的影响,寻找影响肉鸡就巢性的分子标记,为降低或者剔除肉鸡就巢行为的育种研究提供相应依据。采用测序技术对498只清远麻鸡VIPR-1基因5′调控区进行多态性检测及基因型分析,利用最小二乘均数对VIPR-1基因5′调控区的多态位点与就巢性状进行相关分析,利用PHASE软件对多态位点进行单倍型分析。结果,VIPR-1基因5′调控区存在12个多态性位点,G-359T、G-266T、A-134G、A-94G、C-72G对18～43周龄的就巢持续天数影响达到显著水平(P<0.05),A-94G位点对18～54周龄的就巢率的影响达到显著水平(P<0.05);构建的单倍型对18～54周龄的就巢持续天数的影响达到极显著水平(P<0.01)。最小二乘分析结果表明,CCTGGGAAGCAG/TTGGGGAAGCAC型个体的就巢持续天数比其它单倍型个体极显著的延长(P<0.01)。结果表明,VIPR-1基因5′调控区(-496～-1bp)可能存在影响鸡就巢行为的分子标记,CCTGGGAAGCAG/TTGGGGAAGCAC型个体对鸡就巢性具有较大的遗传效应,可作为分子标记应用于家鸡就巢行为的筛选。
基金Supported by the National High Technology Research and Development Program of China (863) (Grant No. 2006AA10Z1E1)
文摘Myostatin is a negative regulator of skeletal muscle mass.The present studycloned the 5' regulatory region of porcine myostatin gene,screened its polymorphisms and analyzedtheir associations with early growth traits in Yorkshire pigs.The results indicated that a fragmentlength polymorphism and a polymorphism concerning two nucleotide changes exist in the 5' regulatoryregion of porcine myostatin gene.At sites 435 and 447,allele A and allele B have the haplotypes ofA-G and G-A,respectively.The allelic frequency of B is 0.475 in Yorkshire pigs.No homozygous BBgenotype was detected in 9 Laiwu Black pigs.Allele B was found to have positive effect on bodyweight on day 21 (BW21) (P【0.01),body weight on day 28 (BW28) (P【0.05),body weight on day 70 (BW70)(P【0.05),average daily gain from birth to 21 d (ADG1) (P【0.01),average daily gain from birth to 28 d(ADG2) (P【0.05) and average daily gain from 21 d to 70 d (ADG3) (P【0.01),respectively.The additiveeffect of allele B on BW21,BW28,BW70,ADG1,ADG2 and ADG3 was 0.596±0.205 kg (P=0.0041),0.498±0.200kg (P=0.0136) 1.409±0.551 kg (P=0.0112),28.39±9.74 g P=0.0041),17.78±7.15 g (P=0.0136) and37.00±16.92 g (P=0.0304),respectively,whereas its effect on average daily gain from 28 d to 70 d(ADG4) was not significant (P】0.1),although BB individuals are superior in average daily gain to AAand AB.
