微量法培养人外周新鲜全血和 PHA 刺激淋巴细胞转化,用~3H-TdR 参入 DNA 合成观测不同浓度亚硒酸钠对转化淋巴细胞增殖的影响。结果表明,低浓度亚硒酸钠(1.18×10-~7mol)促进细胞的增殖,细胞 DNA 合成比对照组平均增高43.5%,而高浓...微量法培养人外周新鲜全血和 PHA 刺激淋巴细胞转化,用~3H-TdR 参入 DNA 合成观测不同浓度亚硒酸钠对转化淋巴细胞增殖的影响。结果表明,低浓度亚硒酸钠(1.18×10-~7mol)促进细胞的增殖,细胞 DNA 合成比对照组平均增高43.5%,而高浓度亚硒酸钠(1.18×10-~5mol)抑制细胞的增殖,细胞 DNA 合成比对照组平均降低93.3%。本实验除表明不同浓度亚硒酸钠对细胞增殖的上述双相作用外,还用回归作图法求得此双相作用的临界亚硒酸钠浓度为1.0×10-~6mol。展开更多
Primary cultures of rat cerebral corticai neurons were prepared from conices or 2 days Sprague-Dawlley rats.We made an attempt to imitate the hypoxic neurons injury for testing neuroprotective erfect or Sibelium. It ...Primary cultures of rat cerebral corticai neurons were prepared from conices or 2 days Sprague-Dawlley rats.We made an attempt to imitate the hypoxic neurons injury for testing neuroprotective erfect or Sibelium. It was found that Sibelium could significantly decrease the amounts of LDH leaking from intracellular fluid and increase the neuron-membrane fluidity of hypoxic neurons.Hypoxic group had significantly higher incorporation of 3H-TDR and 3H-UR than control group,while Sibelium could decrease the incorporation of 3H-TDR and 3H-UR. In addition,Sibelium could decrease the incorporation of 3H-TDR and 3H-UR in normal cultured neurons.The results showed that Sibelium could protect the neuronmembranes against alteration caused by hypoxia and regulate the synthesis of DNA and RNA.展开更多
文摘微量法培养人外周新鲜全血和 PHA 刺激淋巴细胞转化,用~3H-TdR 参入 DNA 合成观测不同浓度亚硒酸钠对转化淋巴细胞增殖的影响。结果表明,低浓度亚硒酸钠(1.18×10-~7mol)促进细胞的增殖,细胞 DNA 合成比对照组平均增高43.5%,而高浓度亚硒酸钠(1.18×10-~5mol)抑制细胞的增殖,细胞 DNA 合成比对照组平均降低93.3%。本实验除表明不同浓度亚硒酸钠对细胞增殖的上述双相作用外,还用回归作图法求得此双相作用的临界亚硒酸钠浓度为1.0×10-~6mol。
文摘Primary cultures of rat cerebral corticai neurons were prepared from conices or 2 days Sprague-Dawlley rats.We made an attempt to imitate the hypoxic neurons injury for testing neuroprotective erfect or Sibelium. It was found that Sibelium could significantly decrease the amounts of LDH leaking from intracellular fluid and increase the neuron-membrane fluidity of hypoxic neurons.Hypoxic group had significantly higher incorporation of 3H-TDR and 3H-UR than control group,while Sibelium could decrease the incorporation of 3H-TDR and 3H-UR. In addition,Sibelium could decrease the incorporation of 3H-TDR and 3H-UR in normal cultured neurons.The results showed that Sibelium could protect the neuronmembranes against alteration caused by hypoxia and regulate the synthesis of DNA and RNA.
