Three new triterpenes, 3β,21α-dihydroxylupen-18(19)-en (1), 3β,25-dihydroxytirucalla- 7,23(24)-dien (2), and 21α-hydroxy-19α-hydrogentaraxastero1-20(30)-en (3) were obtained from an ethyl acetate extr...Three new triterpenes, 3β,21α-dihydroxylupen-18(19)-en (1), 3β,25-dihydroxytirucalla- 7,23(24)-dien (2), and 21α-hydroxy-19α-hydrogentaraxastero1-20(30)-en (3) were obtained from an ethyl acetate extract of the whole plant of Siyekucai (Ixeris chinensis). The structures of 1-3 were determined by spectroscopic methods (EIMS, IR, 1D and 2D NMR).展开更多
Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune cells.This study characterised a high affinity binding receptor of IL-21(IL-21 ...Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune cells.This study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral pathogens.Recombinant CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in E.coli cells,and HEK293 T cells and the native protein from PHA-stimulated kidney leukocytes.In addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium columnare.Our data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.展开更多
文摘Three new triterpenes, 3β,21α-dihydroxylupen-18(19)-en (1), 3β,25-dihydroxytirucalla- 7,23(24)-dien (2), and 21α-hydroxy-19α-hydrogentaraxastero1-20(30)-en (3) were obtained from an ethyl acetate extract of the whole plant of Siyekucai (Ixeris chinensis). The structures of 1-3 were determined by spectroscopic methods (EIMS, IR, 1D and 2D NMR).
基金funded by the National Natural Science Foundation of China(Grant number:32030112)National Key R&D Program of China(Grant number:2018YFD0900302).
文摘Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune cells.This study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral pathogens.Recombinant CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in E.coli cells,and HEK293 T cells and the native protein from PHA-stimulated kidney leukocytes.In addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium columnare.Our data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.